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Extraction and separation method of plant anthocyanin

A separation method and technology for anthocyanins are applied in the field of extraction and separation of plant anthocyanins, and can solve the problems of low purity of anthocyanins, unsatisfactory purity of anthocyanins, etc.

Pending Publication Date: 2022-04-22
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the purity of the anthocyanins purified by the macroporous resin is not ideal enough, and contains other flavonoids with similar polarity to the anthocyanins, so that the purity of the obtained anthocyanins is not high

Method used

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  • Extraction and separation method of plant anthocyanin
  • Extraction and separation method of plant anthocyanin
  • Extraction and separation method of plant anthocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 A kind of separation method of anthocyanins

[0049] 1. Experimental method

[0050] In this example, extracts of Hibiscus roselli were prepared by ethanol extraction and ethyl acetate extraction; strong cation exchange (SCX) column was used to remove other flavonoids to obtain anthocyanin mixture. Anthocyanins were separated and prepared by semi-preparative high-performance liquid chromatography, and 4 kinds of pure anthocyanin monomers (such as figure 1 shown).

[0051] (1) Preparation of anthocyanin mixture

[0052] 1. Preparation of Roselle Extract

[0053] (1) Extraction: select roselle calyx rich in anthocyanin, make it into powder, add acidified ethanol solution for soaking and extraction for 3 times according to mass:volume ratio of 1:5, in which ethanol concentration is 50%-95%, ethanol solution Add one or more of hydrochloric acid, formic acid, and trifluoroacetic acid to adjust the pH value of the ethanol solution to 1-3. The soaking time is ...

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Abstract

The invention discloses a method for extracting and separating plant anthocyanin, which comprises the following steps: extracting plants containing anthocyanin by using an acidified ethanol solution, extracting an extracting solution containing the anthocyanin by using ethyl acetate, purifying the extracting solution by strong cation exchange, and then separating an anthocyanin monomer by high pressure liquid chromatography. According to the method for extracting and separating the anthocyanin disclosed by the invention, flavonoid impurities in the anthocyanin extract are effectively removed; according to the present invention, the four anthocyanin monomers (cyanidin-3-O-glucoside, cyanidin-3-O-moruboside, delphinidin-3-O-glucoside, and delphinidin-3-O-moruboside) can be further successfully separated, and the concentration of each anthocyanin monomer is 97-100%;

Description

technical field [0001] The invention relates to the technical field of extracting plant natural products, in particular to a method for extracting and separating plant anthocyanins. Background technique [0002] Anthocyanins widely exist in the roots, stems, leaves, flowers and fruits of dark plants, making them appear in different colors such as red, purple and blue. They are the main pigment sources of fruits and vegetables such as mulberry, blueberry, roselle and purple sweet potato. Anthocyanins are composed of free anthocyanins (2-benzopyran core) and sugar groups through glycosidic bonds. Anthocyanins generally exist in this form in nature. Anthocyanins belong to flavonoid compounds, and its polyhydroxy structure endows it with unique functionality, so it has the functions of scavenging free radicals in the body, anti-inflammation, and anti-tumor, and also has functional activities such as lowering blood fat and blood sugar (Stintzing F.C., Carle R .(2004).Functional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08C09B61/00C09B67/54
CPCC07H17/065C07H1/08C09B61/00C09B67/0096
Inventor 白卫滨李萍田灵敏吴绮霞孙建霞
Owner JINAN UNIVERSITY
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