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Blackberry extract and application of blackberry extract in preparation of liver cell oxidative damage inhibitor

A technology of extracts and inhibitors, which is applied in the field of blackberry extract and its application in the preparation of liver cell oxidative damage inhibitors, can solve the problems of research, lack of blackberry extract, liver cell oxidative damage, etc., and achieve the goal of expanding the application field Effect

Active Publication Date: 2015-05-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on blackberry extract in protecting liver cells from oxidative damage

Method used

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  • Blackberry extract and application of blackberry extract in preparation of liver cell oxidative damage inhibitor
  • Blackberry extract and application of blackberry extract in preparation of liver cell oxidative damage inhibitor
  • Blackberry extract and application of blackberry extract in preparation of liver cell oxidative damage inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Mix fresh blackberry fruit and water to make a slurry, acidify the blackberry homogenate to pH = 1.5 with 5M HCl, add pepsin (6000 unit pepsin / 20g homogenate), shake in a water bath at 37°C and 300rpm for 3 hours, simulate gastric digestion, Continue to adjust the pH to 6 with 1M NaOH, add 6 mL of pancreatic juice (the content of pancreatic enzyme in pancreatic juice is 4 mg / mL, and the content of bile salt is 40 mg / mL) for every 18 ml of mixed slurry, and then add 1M NaHCO 3 Adjust the pH to 7.4, shake in a water bath at 300 rpm for 2 hours at 37° C., and centrifuge at 6000 rpm for 10 minutes. The supernatant is the crude blackberry extract. Using a Millipore Pellicon ultrafiltration system, the supernatant was pumped to the surface of a regenerated cellulose ultrafiltration membrane with a molecular weight cut-off of 10 kDa, the filtrate was collected, and vacuum freeze-dried to obtain a blackberry extract.

[0035] Tested by liquid chromatography, the main components...

Embodiment 2

[0039] Mix the fresh blackberry fruit with water for beating, acidify the blackberry homogenate with 5M HCl to pH = 2, add pepsin (8000 units of pepsin / 20g homogenate), shake in a water bath at 37°C and 200rpm for 2 hours, after simulating gastric digestion, Continue to use 1M NaOH to adjust the pH to 6.5, add 6mL of pancreatic juice (the content of pancreatic enzyme in pancreatic juice is 4mg / mL, and the content of bile salt is 30mg / mL) for every 18ml of mixed slurry, and then add 1M NaHCO 3 Adjust the pH to 7.4, shake in a water bath at 37°C for 3 hours at 200 rpm, and centrifuge at 8000 rpm for 6 minutes. The supernatant is the crude blackberry extract. Using a Millipore Pellicon ultrafiltration system, the supernatant was pumped to the surface of a regenerated cellulose ultrafiltration membrane with a molecular weight cut-off of 10 kDa, the filtrate was collected, and vacuum freeze-dried to obtain a blackberry extract.

Embodiment 3

[0041] Mix fresh blackberry fruit and water to make a slurry, acidify the blackberry homogenate with 5M HCl to pH = 1.5, add pepsin (7000 units pepsin / 20g homogenate), shake in a water bath at 37°C and 250rpm for 2.5h, simulate gastric digestion , continue to use 1M NaOH to adjust the pH to 7, add 6mL of pancreatic juice (pancreatin content in pancreatic juice is 4mg / mL, bile salt content is 35mg / mL) per 18ml of mixed slurry, and then use 1M NaHCO 3 Adjust the pH to 7.4, shake in a water bath at 37°C for 2.5 hours at 250 rpm, and centrifuge at 7000 rpm for 8 minutes. The supernatant is the crude blackberry extract. Using a Millipore Pellicon ultrafiltration system, the supernatant was pumped to the surface of a regenerated cellulose ultrafiltration membrane with a molecular weight cut-off of 10 kDa, the filtrate was collected, and vacuum freeze-dried to obtain a blackberry extract.

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Abstract

The invention discloses a blackberry extract. The blackberry extract comprises components in percentage by mass as follows: 58.3%-72.5% of cyanidin-3-glucoside, 11.6%-15.8% of cyanidin-3-xyloside, 9.8%-14.6% of cyanidin-3-oxalyl glucoside, 3.1%-6.4% of cyanidin-3-malonyl glucoside, 2.7%-4.1% of delphinidin-3-xylose and 0.3%-0.8% of cyanidin-3-arabinfuranoside. The extraction steps are as follows: after enzymolysis of blackberry fresh fruits by pepsase and trypsin sequentially, centrifugal separation is performed, a liquid supernatant is obtained and filtered by an ultrafiltration membrane, ingredients with molecular weight larger than 10 kDa are cut off, filtrate is subjected to vacuum freezing drying, and the blackberry extract is obtained. The invention further discloses an application of the blackberry extract in preparation of a liver cell oxidative damage inhibitor.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blackberry extract and its application in the preparation of liver cell oxidative damage inhibitors. Background technique [0002] Free radicals are considered to be one of the causes of human aging and certain chronic diseases. When free radicals in the human body are produced too much or cleared too slowly, they will attack and damage various cells and organs, thereby Accelerates the aging process of the body and induces various diseases. [0003] The liver is an important metabolic organ of the human body, and plays the roles of deoxidation, storage of glycogen, and synthesis of secreted proteins in the body. If the liver is damaged, its function will be inhibited. [0004] Acrylamide (AA) is a potential carcinogenic chemical substance, which is produced after high-temperature processing of some starch-rich foods. It is a potential threat to health after being absorbed by the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61K31/7048A61P1/16
Inventor 陈卫鲍涛郑晓冬
Owner ZHEJIANG UNIV
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