Delphinidin-3-O-galactoside separation and purification method and application thereof

A technology of delphinium and galactoside, which is applied in the field of separation and purification of delphinium-3-O-galactoside, and can solve the problem that there is no monomer of delphinidin-3-O-galactoside. Application, no problems such as separation and preparation of delphinidin-3-O-galactoside monomer were found, and the effect of large sample processing capacity and good repeatability was achieved

Active Publication Date: 2018-08-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] However, there is no research and report on the isolation and preparation of delphinidin-3-O-galactoside monomers from blueberries, and no such delphinidin-3-O-galactoside monomers have been used in the preparation of α - Application of glucosidase inhibitors

Method used

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  • Delphinidin-3-O-galactoside separation and purification method and application thereof
  • Delphinidin-3-O-galactoside separation and purification method and application thereof
  • Delphinidin-3-O-galactoside separation and purification method and application thereof

Examples

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Effect test

Embodiment 1

[0072] Wash 1kg of fresh blueberries, add 70% ethanol aqueous solution containing 0.1% (v / v) hydrochloric acid (the volume ratio of ethanol to water is 70 : 30) fully mixed, ultrasonically extracted for 90min, (control temperature below 45°C, protected from light), vacuum filtration after the end of ultrasonication, repeated extraction of the obtained filter residue once according to the above conditions, combined filtrate, and removed by vacuum rotary evaporation at 45°C ethanol to obtain the crude extract of anthocyanins.

[0073] Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized water until the effluent is neutral; then use 0.5M hydrochloric acid solution to rinse for 1 hour at a flow rate of 2BV / h, and then rinse with deionized water until neutral. The crude an...

Embodiment 2

[0078] Wash 5kg of fresh blueberries, add 70% ethanol aqueous solution containing 0.5% (v / v) hydrochloric acid according to the ratio of material to liquid (w / v) 1:7 (w / v) and mix thoroughly, ultrasonically extract for 150min, (control the temperature at 45°C hereinafter, protected from light), vacuum suction filtration after the end of ultrasonication, repeated extraction of the obtained filter residue once according to the above conditions, combined filtrates, and removed ethanol by vacuum rotary evaporation at 45° C. to obtain crude anthocyanin extracts.

[0079]Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized water until the effluent is neutral; then use 0.5M hydrochloric acid solution to rinse for 1 hour at a flow rate of 2BV / h, and then rinse with deionized wa...

Embodiment 3

[0083] Wash 10kg of fresh blueberries, add 60% ethanol aqueous solution containing 0.1% (v / v) hydrochloric acid according to the ratio of material to liquid (w / v) and mix thoroughly, and ultrasonically extract for 200min, (control the temperature at 45°C hereinafter, protected from light), vacuum suction filtration after the end of ultrasonication, repeated extraction of the obtained filter residue once according to the above conditions, combined filtrates, and removed ethanol by vacuum rotary evaporation at 45° C. to obtain crude anthocyanin extracts.

[0084] Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized water until the effluent is neutral; then use 0.5M hydrochloric acid solution to rinse for 1 hour at a flow rate of 2BV / h, and then rinse with deionized water ...

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Abstract

The invention discloses a delphinidin-3-O-galactoside separation and purification method and an application thereof to preparation of an alpha-glucosidase inhibitor. The separation and purification method takes blueberries with complex anthocyanin compositions as raw materials and includes alcohol extraction concentration, macroporous resin adsorption, preparative liquid phase chromatography purification and high-speed counter-current chromatography separation, preparative liquid phase chromatography and high-speed counter-current chromatography technologies are combined, process parameters are optimized, a high-purity delphinidin-3-O-galactoside monomer is prepared by separation, and the purity of the monomer reaches up to 99%. Activity tests discover that the delphinidin-3-O-galactosidemonomer can remarkably inhibit activity of alpha-glucosidase and can be used for preparing the alpha-glucosidase inhibitor.

Description

technical field [0001] The invention relates to the field of separation and purification of natural products, in particular to a method for separation and purification of delphinidin-3-O-galactoside and its application. Background technique [0002] Diabetes is one of the common chronic diseases worldwide. Diabetic patients are usually accompanied by symptoms of hyperglycemia. Long-term hyperglycemia can cause damage to tissues and organs such as nerves, heart, blood vessels and kidneys, and cause a variety of acute and chronic complications. . According to the statistics of the World Health Organization (WHO), there were 422 million diabetic patients in the world in 2014, of which type 2 diabetes is the most common. In recent years, with the changes in people's eating habits and lifestyles and the acceleration of the aging process, the prevalence of diabetes in my country has risen rapidly. By the end of 2016, the number of diabetic patients in my country had reached 110 m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/065A61P3/10
CPCA61P3/10C07H1/08C07H17/065
Inventor 陈卫谢佳宏徐阳谢亮华
Owner ZHEJIANG UNIV
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