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Application of Delphinidin against Staphylococcus aureus

A technology of delphinidin and staphylococcus, which is applied in the fields of application, antibacterial drugs, and medical preparations containing active ingredients, and can solve the problems of no longer having antibiotics

Active Publication Date: 2017-03-01
SAPIOTEC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In particular, due to the increasing use of broad-spectrum antibiotics in the treatment of bacterial infectious diseases and the discovery of more and more bacteria resistant to multiple antibiotics, there is no longer an effective drug in the treatment of patients and animals with these bacterial infections. antibiotics

Method used

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  • Application of Delphinidin against Staphylococcus aureus
  • Application of Delphinidin against Staphylococcus aureus
  • Application of Delphinidin against Staphylococcus aureus

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0063] Preparation of solutions and samples:

[0064] Dilute solution 1: Mixed solution of 100ml methanol and 2.6ml 1M hydrochloric acid

[0065] Dilute solution 2: 100ml 40% methanol and 2.6ml 1M hydrochloric acid mixed solution

[0066] Calibration solution: A reference solution of delphinidin was prepared as follows: 10 mg of delphinidin chloride was weighed into a 10 ml beaker and dissolved into dilution solution 1. After dissolving, it was further diluted about 10 times with Diluent 2 to prepare a solution with a concentration of about 0.1 mg / ml.

[0067] Control calibration solutions will be prepared in the same manner. The calibration solution must then be analyzed immediately by HPLC because delphinidin chloride is not stable in solution.

[0068] Prepare detection solution:

[0069] The delphinium content of the solid prepared in the present invention is determined according to the following scheme (see below for the preparation method): Weigh about 50 mg of the m...

Embodiment 1

[0072] Complex of delphinium and sulfobutyl ether-β-cyclodextrin (SBE-β-CD)

[0073] In this example, delphinidin was mixed with different cyclodextrins to observe the solubility of the complex in aqueous solution.

[0074] Usually, in the prepared neutral aqueous solution, the weight percent of each cyclodextrin is 10. However, when added to the aqueous solution of β-CD, the weight percentage of cyclodextrin is only 2 due to its poor solubility.

[0075] Each 5ml cyclodextrin aqueous solution was put into a glass beaker with corresponding purified water. Subsequently, an excess of delphinidin chloride was added. Wherein, the specific dosage of the excess delphinidin chloride is as follows: 10 mg in α-, β-, γ-cyclodextrin solution, HPBCD (2 hydroxypropyl-β-cyclodextrin) and SBE-β-cyclodextrin 15mg in CD.

[0076] The suspension was stirred for 20 hours at 30°C in the dark. Subsequently, the suspension was filtered using a filter membrane with a pore size of 0.22 μm.

[0...

Embodiment 2

[0081] Effect of pH

[0082] In this example, the effect of pH on the solubility of delphinidin-SBE-β-CD in aqueous solution will be investigated. The SBE-β-CD aqueous solution was prepared according to the method described in Example 1, and then the solution was adjusted to the acidic pH values ​​shown in Table 2 using 1M hydrochloric acid. Subsequently, delphinidin chloride was added to the aqueous solution and further processed according to the method described in Example 1, with the only difference that the stirring time was limited to 2.5 hours. The final results are shown in Table 2.

[0083] pH Delphinidin Chloride 6.0 0.60mg / ml 4.8 2.12mg / ml 4.1 2.03mg / ml

[0084] It can be seen that the solubility of the delphinidin complex in the pH range of 4 to 5 is about 3 times that of the delphinidin complex at neutral pH.

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Abstract

The present invention relates to the application of delphinidin or its salts, and the delphinidin or its salts are aimed at the following bacteria: Staphylococcus aureus showing resistance to antibiotics and aureus showing low sensitivity to antibiotics Staphylococcus aureus, and can at least partially neutralize said bacteria. In addition, the present invention also relates to a method of treating individuals with delphinidin or its salts.

Description

technical field [0001] The present invention relates to the application of anthocyanin delphinium pigment and its salts for combating bacteria resistant to antibiotics and bacteria showing low sensitivity to antibiotics. Background technique [0002] Anthocyanins are cytochrome dyes present in most tall land plants. Anthocyanins contain no sugars (aglycones) and are closely related to sugar-containing anthocyanins (glycosides), both of which fall under the general heading of anthocyanins. Anthocyanins are dyes with antioxidant properties. [0003] Antibiotic-resistant bacteria and antibiotic-hyposusceptible bacteria are often found in places where antibiotics are frequently used. Antibiotic-resistant Staphylococcus aureus and antibiotic-hyposensitivity Staphylococcus aureus are among the most dangerous hospital-acquired pathogens, ie nosocomial infections, which can be transmitted not only through the skin and mucous membranes but also through Infected medical devices or ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A61K31/352A01P1/00A61P31/04A23L3/3544A23K10/20A23K10/30A23K20/00A23K20/121A23K20/163A23K20/195
CPCA61K8/498A61Q17/005A61K8/738A61K2800/56A01N43/16A61P11/00A61P15/14A61P17/00A61P21/00A61P31/00A61P31/04A61P9/00A01N25/02A01N25/22A61K31/352A61K9/08A61K9/14
Inventor 诺伯特·若维尔延斯·布若斯凯特
Owner SAPIOTEC
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