Method for promoting accumulation of anthocyanin in roselle calyxes

A technology of roselle calyx and anthocyanin, which is applied in the field of plant cultivation, can solve the problems such as the ineffective accumulation of anthocyanin, and achieve the effect of reliable anthocyanin food raw materials, better effect, and simple and easy preparation method

Inactive Publication Date: 2016-03-09
ANQING NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, the above methods have no obvious effect on the accumulation of anthocyanins in roselle calyx, so how to promote the accumulation of anthocyanins in roselle still needs to be explored

Method used

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  • Method for promoting accumulation of anthocyanin in roselle calyxes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 composition

[0017] In the composition of the present invention, white granulated sugar accounts for 15% (mass percentage), uniconazole 80ppm, inducer GA350mg / L, surfactant 0.2%, L-phenylalanine 400-800ppm, coumaric acid 50 -90ppm, dihydroflavonol 5-15ppm, in order to realize optimal proportioning, the present invention designs three factor three level orthogonal experiments to the proportioning of L-phenylalanine, coumaric acid and dihydroflavonol, See Table 1 for the experimental design.

[0018] The orthogonal test design table of three kinds of plant growth regulators in the composition of table 1

[0019]

[0020] Using the method provided in Example 3, measure the content of anthocyanins in roselle after the treatment of different proportioning compositions. As a result (Table 1), it can be seen that the scheme of A1B3C3 is optimal, and the combination prepared according to this proportioning The anthocyanin content reached 25...

Embodiment 2

[0022] Embodiment 2 The using method of this composition

[0023] The test was carried out from May 17th to June 3rd, 2014. In the orchard, 20 roselle plants with similar growth potential and loading capacity were selected, and clear water was used as the control. Roselle is treated, and the specific method is: use the composition to smear the receptacle and flower stalk every day from the first day of the budding period until the full blooming period, after that, use the spray method to treat the whole flower buds, and after the full blooming period, apply the composition on the For the middle part of the fruit stem, stop processing three days before picking.

Embodiment 3

[0024] The assay method of embodiment 3 anthocyanins

[0025] For anthocyanins, refer to Jiang Meiqiao's method (see "Preliminary Study on the Law of Accumulation of Anthocyanin Content in Purple Sweet Potato").

[0026] (1) Reagents and configuration methods

[0027] (1) 0.025mol / L pH1.0 hydrochloric acid-potassium chloride buffer solution; 1.86g KCL was dissolved in 980mL distilled water, adjusted to pH 1.0 with concentrated hydrochloric acid, and the volume was set to 1000mL.

[0028] (2) 0.4mol / L pH4.5 hydrochloric acid-sodium acetate buffer solution, 54.43g crystalline sodium acetate was dissolved in 960mL distilled water, the pH was adjusted to 4.5 with concentrated hydrochloric acid, and the volume was set to 1000mL.

[0029] (3) Hydrochloric acid ethanol solution, 95% ethanol: 1.5mol / L hydrochloric acid, mixed in a volume ratio of 85:15.

[0030] (2) Raw material processing

[0031] The test samples were weighed three times for each treatment. 100g calyx was homoge...

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Abstract

The invention belongs to the field of plant cultivation, and particularly relates to a method special for increasing the content of anthocyanin in roselle. In order to increasing the content of the anthocyanin in the roselle, a composition is supplied for the glucoside synthesizing process of delphinidin and cyaniding in the roselle, all the components in the composition serve as precursors and intermediate products which are necessary in biosynthesis of the roselle anthocyanin, and through continuous exploration and improvement, the best using time and applying method of the composition are determined. It is determined that the content of the anthocyanin in roselle calyxes can be increased by multiple times through the method, and the content of the anthocyanin in the calyxes is 121%-293% of that of a control group; in addition, no pollution is generated to the environment, and higher-quality raw materials can be supplied to anthocyanin processing industry through the method.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for specially increasing the anthocyanin content in roselle. Background technique [0002] Roselle is an annual herb of the Malvaceae hibiscus genus, also known as red horned sunflower, roselle, mountain eggplant, Sudan red, merina, etc. It is native to the West Indies of Africa and has been planted for hundreds of years. Widely distributed in tropical and subtropical regions of the world. At present, my country's Guangdong, Guangxi, Yunnan, Fujian, Zhejiang, Sichuan, Jiangxi and other provinces have large-scale planting and cultivation. Its calyx, seeds and leaves can be used, and it is a tropical and subtropical economic crop with multiple economic uses. Roselle is rich in nutrients and has unique functions. The calyx is a fleshy structure, juicy, rich in protein, organic acids, various amino acids, a large amount of natural pigments and minerals needed b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01G1/00A01N43/653A01N37/44A01N37/06A01N43/16A01N43/12A01P21/00
CPCA01G7/06A01G22/00A01N37/06A01N37/44A01N43/12A01N43/16A01N43/653A01N2300/00
Inventor 吴娟穆丹陈金苗许远
Owner ANQING NORMAL UNIV
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