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Microbial agent, preparation method thereof and application thereof

A technology of microbial agent and bacilli, which is applied in the field of microbial agent and its preparation, can solve the problems of incomplete promotion, development and application of microbial agent, low product promotion rate, scattered product types, etc., so as to facilitate industrial promotion and application, Improved stability and easy operation

Active Publication Date: 2019-01-11
陕西赛恩农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main domestic research is still focused on the selection and cultivation of high-efficiency strains, and the promotion, development and application of microbial agents are still not perfect
Although there are many research results and patents related to microbial agents, the product categories are still very scattered, and the product treatment effect is affected by many factors. The effect is single, the effect is unstable, and the product promotion rate is still not high.

Method used

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  • Microbial agent, preparation method thereof and application thereof
  • Microbial agent, preparation method thereof and application thereof
  • Microbial agent, preparation method thereof and application thereof

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preparation example Construction

[0056] According to a second aspect of the present invention, a method for preparing a microbial inoculum is provided, comprising the following steps:

[0057] After mixing the respective fermentation products of Streptomyces albicans CGMCC No.13717, Paecilomyces lilacinus CGMCC No.13898 and Streptomyces extremely longue with the initially fixed mixed bacteria fermentation products according to the ratio, carry out secondary fixation to obtain microbial bacteria agent.

[0058] Immobilized microorganism technology overcomes the disadvantages that the biological cells are too small, difficult to separate from the solution, and easily cause secondary pollution. The immobilization methods of microorganisms include adsorption method, covalent binding method, embedding method and cross-linking method, among which embedding method is the most commonly used. The primary immobilization and secondary immobilization in the preparation method of the microbial bacterial agent disclosed i...

Embodiment 1

[0096] A microbial agent, including Streptomyces albicans CGMCC No.13717, Paecilomyces lilacinus CGMCC No.13898, Streptomyces extremely longus and mixed bacteria; mixed bacteria include yeast, Bacillus subtilis, Bacillus licheniformis, amyloliquefaciens Bacillus, Brevibacillus serospora, and Trichoderma harzianum;

[0097] Among them, the proportion of viable bacteria of Streptomyces albicans CGMCC No. 13717, Paecilomyces lilacinus CGMCC No. 13898, Streptomyces elongatus and mixed bacteria is 25:14:18:6;

[0098] The ratio of live bacteria of yeast, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Brevibacillus spores and Trichoderma harzianum in the mixed bacteria is 15:5:7:1:7:2;

[0099] The total viable bacterial concentration of the microbial agent is about 2×10 8 CFU / mL.

[0100] The preferred preparation method of above-mentioned microbial bacterial agent, comprises the following steps:

[0101] (1) Inoculate the preserved Streptomyces albicans,...

Embodiment 2

[0109] A microbial agent, including Streptomyces albicans CGMCC No.13717, Paecilomyces lilacinus CGMCC No.13898, Streptomyces extremely longus and mixed bacteria; mixed bacteria include yeast, Bacillus subtilis, Bacillus licheniformis, amyloliquefaciens Bacillus, Brevibacillus serospora, and Trichoderma harzianum;

[0110] Among them, the proportion of viable bacteria of Streptomyces albicans CGMCC No. 13717, Paecilomyces lilacinus CGMCC No. 13898, Streptomyces elongatus and mixed bacteria is 10:30:7:20;

[0111] The ratio of live bacteria of yeast, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Brevibacillus spp. and Trichoderma harzianum in the mixed bacteria is 7:8:3:9:2:8;

[0112] The total viable bacterial concentration of the microbial agent is about 3.5×10 8 CFU / mL.

[0113] The preferred preparation method of above-mentioned microbial bacterial agent, comprises the following steps:

[0114] (1) Inoculate the preserved Streptomyces albicans, ...

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Abstract

The invention discloses a microbial agent, a preparation method thereof, and an application thereof, which relate to the technical field of microbial agent. The microbial agent includes Streptomyces albidofuscus CGMCC No.13717, Paecilomyces lilacinus CGMCC No. 13898, Streptomyces longus and mixed bacteria, wherein the mixed bacteria includes yeast, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Brevibacillus laterosporus and Trichoderma harzianum. Through combination of bacteria-fungi and the synergistic effect of multi-strains, the microbial agent has the advantages ofhigh activity of strains, high efficiency and multi-functions in treating waste water, waste gas or waste. In addition, the invention also provides a preparation method of a microbial agent, which adopts a double-layer immobilization (preliminary immobilization and secondary immobilization combination) method of the microbial agent to improve the stability of the microbial agent. The preparationmethod is simple in operation and easy to be popularized and applied in industry.

Description

technical field [0001] The invention relates to the technical field of microbial inoculum, in particular to a microbial inoculum and its preparation method and application. Background technique [0002] With the continuous development of science and technology and production technology, a large number of artificially synthesized compounds have entered the environment. Due to their complex structure and biological strangeness, they are difficult to be decomposed and utilized by microorganisms in a short period of time. The types and quantities of microorganisms with high-efficiency degradation ability for such pollutants in the natural environment are relatively small, and they are at a disadvantage in interspecies competition. Bioaugmentation technology can give full play to the potential of microorganisms and improve the biological treatment effect of refractory organic matter. This technology has the characteristics of high efficiency, stability and investment saving, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C12N11/10C12N11/08C02F3/34B09C1/10C12R1/465C12R1/79C12R1/07C12R1/10C12R1/125C12R1/885C12R1/01
CPCC12N1/14C12N1/16C12N1/20C12N11/08C12N11/10C02F3/34C02F3/347B09C1/10B09C1/105
Inventor 陈琳李红超
Owner 陕西赛恩农业科技股份有限公司
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