Microecological preparation-tetragenous viable bacteria preparation and method for preparing same

A live bacteria preparation and live bacteria technology, applied in the field of micro-ecological preparations, can solve the problems of unsustainable effect and single effect

Inactive Publication Date: 2006-10-11
江苏省苏微微生物研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Single-strain type, such as photosynthetic bacteria preparations, bacillus preparations, etc., contains only one kind of microorganism, and has no advantages of synergistic effect of multiple strains. Relatively speaking, there are limitations of single effect and short-lasting effect

Method used

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  • Microecological preparation-tetragenous viable bacteria preparation and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1: the pure culture of living thalline

[0117] (1) Purebred culture of Bacillus subtilis CGMCC1.1414

[0118] Activation of strains

[0119] Aseptically open the freeze-dried strain of Bacillus subtilis, streak and inoculate on the slant of nutrient broth agar in the test tube, incubate at 37°C for 18-24h, then transfer to the slant of nutrient broth agar eggplant bottle, and incubate at 37°C for 20-24h , Microscopic examination, when more than 90% of the bacteria form spores, it is mature and ready to transplant.

[0120] seed culture

[0121] Prepare a sterile triangular flask with glass beads inside, scrape and wash the mature sludge on the slope of the eggplant bottle with sterile water, put it into the triangular flask, shake and disperse the sludge to obtain a uniform bacterial suspension. Heat the bacterial suspension in a water bath at 85°C for 5 minutes, and inoculate 0.1m with a volume ratio of 5%. 3 Seed cans. The filling factor of the seed ca...

Embodiment 2

[0156] Embodiment 2: the mixed culture of clostridium butyricum, Lactobacillus casei and yeast live thalline

[0157] Activation of strains

[0158] The activation of Clostridium butyricum, Lactobacillus casei, and saccharomyces freeze-dried strains is the same as described in Example 1.

[0159] seed culture

[0160] The seed culture of Clostridium butyricum, Lactobacillus casei, and saccharomyces is the same as described in Example 1.

[0161] fermenter culture

[0162] Inoculate the seed culture solution of Clostridium butyricum, Lactobacillus casei, and yeast at 6%, 12%, and 2% inoculation amounts into 1 m 3 Fermenter with a filling factor of 80% (v / v).

[0163] The medium composition of the fermenter is: brown sugar 20g, peptone 5g, yeast extract 3g, KH 2 PO 4 1g, (NH 4 ) 2 SO 4 2g, NaCl 3g, CaCO 3 3g, NaAc 3g, tap water 1000ml, pH 7.0, anaerobic culture at 35°C for 48h.

[0164] Cell collection

[0165] The culture solution is pumped into a continuous cent...

Embodiment 3

[0166] Embodiment 3: the preparation of the original bacterial powder of microbial pure strain

[0167] (1) Bacillus subtilis original bacteria powder

[0168] Mix the wet bacteria of Bacillus subtilis collected after pure breeding with dry starch at a mass ratio of 1:2, dry at 40-50°C for 20-24 hours at low temperature, pulverize with a pulverizer, pass through a 40-mesh sieve, and adjust with dry starch The total number of live bacteria of Bacillus subtilis in the original bacteria powder is ≥5×10 8 CFU / g.

[0169] (2) Clostridium butyricum original bacteria powder

[0170] Mix the wet bacteria of Clostridium butyricum collected after the pure culture with dry starch at a mass ratio of 1:2.5, dry at 40-50°C for 20-24 hours at low temperature, pulverize with a pulverizer, pass through a 40-mesh sieve, and use a dry The total number of live bacteria of Clostridium butyricum in the starch-adjusted original bacteria powder is ≥5×10 8 CFU / g.

[0171] (3) Lactobacillus casei ...

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Abstract

This invention relates to a micro-ecological agent and the preparation method thereof, belonging to the micro-ecological agent technological field. The preparation of the active bacteria agent of this invention comprises: using L-calcii lactas and four useful micro-organisms of clostridium bytyricum, Lactobacillus casei, bacillus subtilis and saccharomycete as characteristic elements, cultured purely or mixedly by means of universal fermentator, producing active bacteria powder by freeze-drying, and acquiring the finished products by double crossing of the powder at a specific proportion. By using the anaerobic and aerobic characteristics of related bacterial strains, this agent has the following merits: playing the synergy effect in enhancing the disease resistance of marine animals and the water purification of aquaculture, substituting antibiotics fully or partly for the disease prevention and cure in fresh water aquiculture and mariculture, and improving the economic benefits of aquaculture. The L-calcii lactase component can accelerate the husking growth and prevent the disease of soft husk of shellfish marine animals like shrimp and crab. This agent is characterized of being nontoxic, free from side effects, nonpersistent, without secondary pollution and no drug tolerance, and will have a wide commercial application in the fresh water aquiculture mariculture.

Description

technical field [0001] A micro-ecological preparation-quadruple live bacteria preparation and a preparation method thereof, which belong to the technical field of micro-ecological preparations. technical background [0002] In recent years, with the rapid development of my country's aquaculture industry and the increasing scale of high-density and intensive aquaculture, high concentrations of aquatic animal excreta in the aquaculture water, bait residues caused by a large amount of bait and arbitrary discharge of sewage have caused The pollution of the natural waters has made the breeding ecological environment worse. Aquatic animals, especially crustaceans such as shrimps and crabs, have a low immune system function and mainly rely on non-specific substances produced by various blood cells such as cohesin lysozyme to exert the body's non-specific immune function. Attacked by pathogenic bacteria and viruses, various diseases occur frequently and reduce the economic benefits ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12N1/16C12R1/07C12R1/125C12R1/245
Inventor 陆茂林孙梅匡群施大林刘淮何义进
Owner 江苏省苏微微生物研究有限公司
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