Composite micro-ecological preparation for aquatic product and premix thereof

A technology of composite microecology and preparation, applied in the field of applied biology, can solve the problems of not being able to adapt to the complex and changeable water environment, restricting the promotion and application of microecological preparations, and unstable performance of preparation strains, so as to achieve good reproductive ability and optimize aquatic products. The effect of breeding environment and good micro-ecological regulation

Active Publication Date: 2012-07-11
江苏大北农水产科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are already a variety of compound microecological preparations on the market that can achieve obvious effects in one or some aspects of water quality improvement and disease prevention, but many preparations still have problems such as unstable strain performance, single function and poor environmental adaptability. It cannot adapt to the complex and changeable environment of the water body, and the effect is unstable after being applied to the water body, thus limiting the promotion and application of probiotics in aquaculture

Method used

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  • Composite micro-ecological preparation for aquatic product and premix thereof
  • Composite micro-ecological preparation for aquatic product and premix thereof
  • Composite micro-ecological preparation for aquatic product and premix thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The preparation of the high-density fermented liquid of embodiment 1 three kinds of bacteria and liquid composite probiotics

[0022] 1. Preparation of high-density fermentation broth of Enterococcus faecalis CGMCC No.5092

[0023] 1) Plate culture rejuvenation: inoculate Enterococcus faecalis on the MRS plate medium and culture at 37°C for 18 hours to rejuvenate Enterococcus faecalis and form a single colony, pick a single colony on the inoculation medium, and culture at 37°C 24h;

[0024] 2) Preparation of primary seeds: transfer the Enterococcus faecalis cultured in step 1) into a 2L shaker flask filled with 300 ml of MRS liquid medium, and culture at 37° C. for 24 hours until late logarithm to obtain primary seeds;

[0025] 3) Preparation of secondary seeds: Transfer the primary seeds prepared in step 2) to a 100L seed tank equipped with 80LMRS seed medium, cultivate at a temperature of 37° C. and a rotational speed of 60 rpm for 12 hours to obtain a secondary seed...

Embodiment 2

[0042] The preparation of embodiment 2 probiotics granule of the present invention

[0043] Get the fermented liquid of 1,2,3 in Example 1 respectively by volume ratio and carry out centrifugation, its ratio is: Enterococcus faecalis: Bacillus licheniformis: Bacillus subtilis=2: 1: 1, centrifugal speed is 15000rpm, after centrifugal The obtained bacteria sludge is mixed, and then 25% of the carrier of the total volume of the three fermentation broths before centrifugation is added to mix with the bacteria sludge, and the rotary extrusion granulation method is used for granulation, and the drying is carried out through a vibrating fluidized bed. 70°C, air outlet 35°C, feed rate 120kg / h; the moisture content is 8.5% microecological preparation granules, and the number of viable bacteria is 3.5×10 10 cfu / g.

Embodiment 3

[0044] Example 3 Effects of the probiotics of the present invention on the activity of crucian carp digestive enzymes and intestinal flora

[0045] 180 fish fingerlings were randomly divided into 3 groups, each group had 3 repetitions, and were fed with 3 kinds of feeds: basal ration (group I), added in basal ration (composition and nutrient level as shown in Table 1) 0.02% by mass of the compound microecological granule preparation (group II) in Example 2, and 0.02% by mass of the compound microecological liquid (group III) was added to the basal diet. The volume of the test aquarium is 0.85m×0.45m×0.55m, and 20 fish are thrown into each box.

[0046] Table 1 Composition and nutritional level of crucian carp basal diet

[0047]

[0048]

[0049] Note: Additives contain trace elements and vitamins, etc.

[0050] Preparation of the test group feed: pulverize the above-mentioned raw materials, and mix them thoroughly after passing through a 60-mesh sieve. The compound mi...

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Abstract

The invention belongs to the technical field of application biology and relates to a composite micro-ecological preparation for an aquatic product and a premix thereof. The efficient and multifunctional composite micro-ecological preparation is formed by combining multiple beneficial strains having different functions in a certain proportion; the preparation can obviously promote individual weight increment of an aquatic animal and improve yield per unit area and total yield, thereby increasing the income of aquaculture and improving benefits; and the preparation provided by the invention has the effects in multiple aspects of improving water quality, increasing algae in water, inhibiting harmful flora, improving the environment in the intestinal tract of an aquaculture animal, increasing feed and the like.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, and relates to a composite microecological preparation for aquatic products and a premix thereof. Background technique [0002] With the continuous improvement of aquaculture intensification, the physical and chemical environment and ecological environment of aquaculture water are deteriorating; at the same time, the excessive discharge of human domestic sewage, various industrial wastewater, and agricultural wastewater has exacerbated the decline in the quality of aquaculture water; these problems As a result, breeding diseases are becoming more and more serious; at the same time, people's living standards have improved, food safety awareness has increased, and the requirements for animal-derived products have become more and more standardized after joining the WTO. When producing and using fishery medicines, it is essential to consider Whether the fishery medicine meets the food safety requ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/10A23K1/16C02F3/34C12R1/125C12R1/10C12R1/01
Inventor 杜建涛付维来吴雅琨李晓清王安如
Owner 江苏大北农水产科技有限公司
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