Using Alternating Electric Fields to Increase Cell Membrane Permeability

a technology of cell membrane and electric field, which is applied in the direction of contraceptive devices, drug compositions, and therapy, can solve the problems of perturbing mitosis, promoting final impaired cellular proliferation, etc., and achieves the effects of reducing the viability of cancer cells, and increasing the permeability of the cell membran

Pending Publication Date: 2020-01-09
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV +1
View PDF6 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008]Another aspect of the invention is directed to a second method for attacking cancer cells. The second method comprises applying a first alternating electric field at a first frequency to the cancer cells for a first period of time, wherein application of the first alternating electric field at the first frequency to the cancer cells for the first period of time increases permeability of cell membranes of the cancer cells; introducin

Problems solved by technology

Such stress ultimately promotes impaired cellular proliferation.
For instance, TTFields has been shown to disrupt the localization o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Using Alternating Electric Fields to Increase Cell Membrane Permeability
  • Using Alternating Electric Fields to Increase Cell Membrane Permeability
  • Using Alternating Electric Fields to Increase Cell Membrane Permeability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047]As used herein, the term “reducing viability” of a cell refers to reducing the growth, proliferation, or survival of the cell, or increasing cytotoxicity of the cell.

[0048]This application describes a novel approach for temporarily increasing the permeability of the plasma cell membranes of cancer cells using alternating electric fields so that substances that are ordinarily blocked by the cell membrane will be able to cross the cell membrane, or so that substances that are ordinarily impeded by the cell membrane will be able to cross the cell membrane more easily. In some of the examples described herein, this approach is used for temporarily increasing the permeability of glioblastoma plasma cell membranes using alternating electric fields so that substances that are ordinarily impeded by the glioblastoma cell membrane will be able to cross the glioblastoma cell membrane more easily.

[0049]The inventors have demonstrated that TTFields treatment, in conjunction with a novel an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This Application claims the benefit of U.S. Provisional Applications 62 / 693,811 (filed Jul. 3, 2018), 62 / 728,255 (filed Sep. 7, 2018), and 62 / 795,136 (filed Jan. 22, 2019), each of which is incorporated herein by reference in its entirety.BACKGROUND[0002]The treatment of glioblastoma (GBM) using alternating electric fields is a novel, validated therapy that has become an additional modality (after surgery chemoradiation and chemotherapy) for anti-cancer treatments. Intermediate frequency alternating electric fields (100-500 kHz) have been studied in detail. Most recently, TTFields has been shown to prolong median survival (by 5 months) of glioblastoma patients on maintenance temozolomide chemotherapy. In the context of treating tumors, alternating electric fields at these frequencies are often referred to as “tumor treating fields” or “TTFields.”[0003]Many hypotheses on TTFields' mechanism exist, but the most widely proposed (“standard”) ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61N1/32
CPCA61N1/327A61N1/36002A61N1/3603A61N1/0412A61N1/025A61P35/00A61K41/0061A61M37/00A61M2037/0007
Inventor CHANG, EDWINPATEL, CHIRAG B.GAMBHIR, SANJIV S.VOLOSHIN-SELA, TALIPORAT, YAARAGILADI, MOSHE
Owner THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap