Method for inducing polyploid miscanthus sinensis

A technology of polyploidy and body awn, applied in the field of plant cytogenetics, can solve the problems of high price, high callus toxicity, and increased mutagenesis cost, and achieve the effect of low toxicity, high induction rate and low cost

Active Publication Date: 2014-04-16
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Colchicine is a commonly used polyploid mutagen, but it was found during the polyploid mutagenesis of awns that it is very toxic to the callus of awns. After the mutagenesis treatment, the embryogenic cells often Abnormal death of tube polymerization leads to low polyploid mutagenesis rate; in addition, colchicine is expensive, usually around 1,000 yuan / g, which increases the cost of mutagenesis when polyploids are induced in large-scale factories

Method used

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  • Method for inducing polyploid miscanthus sinensis
  • Method for inducing polyploid miscanthus sinensis

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Experimental program
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Embodiment 1

[0031] Material: Miscanthus (M.sinensis) 02178, collected from Huaihua, Hunan, and preserved in the Miscanth plant resource nursery of Hunan Agricultural University.

[0032] 1. Preparation of mutagenic materials: select the induced compact embryogenic callus with bud points, and collect them in a 50ml Erlenmeyer flask for later use;

[0033] 2. Mutation of polyploid: soak embryogenic callus in mutagen, mutagen components include 25g / L mannitol, 1.02mM Ca 2+ and 10 μM asulphin, the solvent is water; the treatment temperature is 5°C, the treatment is 15 minutes, and 5 repetitions are set; the callus is taken out, inoculated into hormone-free basic medium, cultured in the dark at 5°C, and treated for 36 hours.

[0034] 3. Cluster bud induction or proliferation: Take out the treated callus, wash it with 25g / L mannitol solution sterilized three times, once for 2 minutes; after cleaning, absorb it with sterilized filter paper Dry the residual solution; transfer the callus to the d...

Embodiment 2

[0038] Material: Miscanthus (M.sinensis) 00051, collected from Leshan, Sichuan, and preserved in the Miscanth plant resource nursery of Hunan Agricultural University.

[0039] 1. Preparation of mutagenic materials: select the induced compact embryogenic callus with bud points, and collect them in a 50ml Erlenmeyer flask for later use;

[0040] 2. Mutation of polyploid: soak embryogenic callus in mutagen, mutagen components include 20g / L mannitol, 1.02mM Ca 2+ and 5 μM nocodazole, the solvent is water; the treatment temperature is 15°C, the treatment is 20 minutes, and 5 repetitions are set; the callus is taken out, inoculated into hormone-free basic medium, cultured in the dark at 15°C, and treated for 48 hours.

[0041] 3. Cluster bud induction or proliferation: Take out the treated callus, wash it with 20g / L mannitol solution sterilized 5 times, once for 1 minute; after cleaning, absorb it with sterilized filter paper Dry the residual solution; transfer the callus to the di...

Embodiment 3

[0045] Material: Miscanthus (M.sinensis) 02178, collected from Huaihua, Hunan, and preserved in the Miscanth plant resource nursery of Hunan Agricultural University.

[0046] 1. Preparation of mutagenic materials: select the induced compact embryogenic callus with bud points, and collect them in a 50ml Erlenmeyer flask for later use;

[0047] 2. Mutation of polyploid: soak embryogenic callus in mutagen, mutagen components include 25g / L mannitol, 2.99mM Ca 2+ and 5 μM nocodazole, the solvent is water; the treatment temperature is 25°C, the treatment is 15min, and 5 repetitions are set; the callus is taken out, inoculated into hormone-free basic medium, cultured in the dark at 25°C, and treated for 36h.

[0048] 3. Cluster bud induction or proliferation: Take out the treated callus, wash it with 25g / L mannitol solution sterilized three times, once for 2 minutes; after cleaning, absorb it with sterilized filter paper Dry the residual solution; transfer the callus to the differenti...

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Abstract

The invention discloses a method for inducing polyploid miscanthus sinensis. According to the invention, polyploid mutation is carried out by utilizing a miscanthus sinensis sprout point callus; a mutagenic agent is a water solution which contains Ca<2+> and mannitol, and the water solution also comprises oryzalin or nocodazole which is already subjected to filter sterilization; and finally obtained polyploid mutation rate can achieve 35.19% and a period is 2-3 months through polyploid identification. Compared with conventional breeding, the method disclosed by the invention greatly shortens the mutation time, greatly enhances the mutation rate and greatly reduces the cost; and the method disclosed by the invention provides a new way for selectively breeding a new miscanthus sinensis species.

Description

technical field [0001] The invention belongs to the field of plant cytogenetics, and relates to plant tissue culture technology, in particular to the induction technology of polyploid awn. Background technique [0002] Miscanthus sinensis belongs to the genus Miscanthus Andresson of the Poaceae family. It is a tall perennial herb that is distributed all over the country except in Northwest China and Tibet. Due to its wide ecological adaptability, high energy efficiency, diverse economic uses and strong environmental protection functions, it has become one of the most concerned biomass energy sources. To put it into industrial production, in addition to breaking the relevant technical barriers, the quality and quantity of raw materials are also the top priority. Because plant polyploid has the characteristics of huge shape, strong stress resistance, and high content of chemical substances, it has been widely valued by breeders. Mang×tetraploid Hag) has been studied more dee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈智勇易自力刘清波
Owner HUNAN AGRICULTURAL UNIV
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