13 results about "Miscanthus sinensis" patented technology

The present invention provides a deep eutectic solvent and its preparation and application. The deep eutectic solvent comprises choline chloride, p-toluenesulfonic acid, 1,4-butanediol, and water; wherein the molar ratio of choline chloride, p-toluenesulfonic acid, and 1,4-butanediol is 1-2:3-6:2-8, and the ratio of the total mass of choline chloride and p-toluenesulfonic acid to the mass of water is 20:9-11. When used for the pretreatment of Miscanthus sinensis, the deep eutectic solvent of the present invention can effectively remove hemicellulose and lignin from the cell walls of Miscanthus sinensis, thereby significantly improving the efficiency of its enzymatic hydrolysis of cellulose.

The invention belongs to the technical field of soil microbial control, and discloses a compound microbial agent for treating mine tailing polluted soil, the compound microbial agent comprises a coxsackie P1-4-containing bacterial liquid and a stenotrophomonas R5-containing bacterial liquid in a volume ratio of (1-2): (1-2), and the OD600 value of the coxsackie P1-4-containing bacterial liquid and the OD600 value of the stenotrophomonas R5-containing bacterial liquid are independently 0.5-1.5. The invention further discloses a preparation method of the complex microbial inoculant and application of the complex microbial inoculant in treatment of mine tailing polluted soil. The coxakia P1-4 and the stenotrophomonas R5 screened by the invention are both derived from a cadmium-resistant environment (rhizosphere of miscanthus sinensis and endogenous rice), and have natural tolerance to heavy metal stress; the microbial agent prepared by compounding the microbial agent shows extremely strong adaptability and repair pertinence to typical acidic and cadmium-lead combined polluted soil in mine tailing areas, and solves the general problems of low survival rate and function failure of a common microbial agent in a severe tailing environment.

This invention discloses a gene, MsDHN1, that regulates salt tolerance in Miscanthus sinensis, the nucleotide sequence of which is shown in SEQ ID No. 1. The amino acid sequence of the protein product expressed by MsDHN1 is shown in SEQ ID No. 2. This invention knocked down the expression level of the DNH gene in plants using VIGS. Under saline-alkali treatment, the plants exhibited a certain degree of wilting, reduced survival rate, and decreased fresh weight and chlorophyll content, indicating that this gene plays a positive regulatory role in the plant's salt stress response. This gene has important application value in the field of salt tolerance breeding for the energy plant Miscanthus sinensis.

The present invention belongs to the field of agricultural planting technology and specifically relates to a method based on 60 The method for cultivating salt-tolerant Miscanthus sinensis mutants induced by Co-γ radiation comprises the following steps: S1 seed material disinfection: drying Miscanthus sinensis seeds and then soaking them, washing them after soaking, adding a disinfectant to soak and disinfect them, washing and removing water after disinfection to obtain sterile seed materials; S2 radiation mutagenesis: inoculating the sterile seed materials onto a germination medium, selecting 60 Co-γ radiation induces mutation in seed material, and the mutated seed material is transplanted into a screening medium for screening and cultivation to germinate salt-tolerant Miscanthus sinensis seedlings; S3 Miscanthus sinensis seedling cultivation: the salt-tolerant Miscanthus sinensis seedlings are transplanted into a seedling-strengthening medium for cultivation until the seedling buds emerge and then transplanted; S4 Miscanthus sinensis seedling transplanting: the salt-tolerant Miscanthus sinensis seedlings in S3 are transplanted into a cultivation medium for cultivation to obtain salt-tolerant Miscanthus sinensis mutants. The Miscanthus sinensis cultivated by the present invention is cultured in a special culture medium and cultivation medium to ensure the germination rate and survival rate of the mutants, thereby cultivating a Miscanthus sinensis mutant with strong salt tolerance.

The invention discloses an SNP molecular marker for identifying reed roots and application of the SNP molecular marker, and belongs to the technical field of molecular biological detection. The SNP molecular marker comprises a nucleotide sequence as shown in SEQ ID NO.1, wherein the 36th polymorphism of the nucleotide sequence is A or G, the 80th polymorphism of the nucleotide sequence is A or G, the 140th polymorphism of the nucleotide sequence is T or G, and the 222nd polymorphism of the nucleotide sequence is G or A. The SNP molecular marker capable of efficiently distinguishing the reed roots from the reed roots, the miscanthus sinensis and the triarrhena lutarioriparia roots is obtained by comparative analysis of genome sequences of the reed roots, the reed roots, the miscanthus sinensis and the triarrhena lutarioriparia roots, and further efficient identification of the reed roots is achieved through genotypes of different SNP loci in the SNP molecular marker. The SNP molecular marker has a good application prospect in identification of reed roots.

The invention discloses a compound microbial agent for ensiling miscanthus sinensis and application of the compound microbial agent. The microbial agent is prepared from lactobacillus plantarum LW8 (CGMCC NO.28108) and lactobacillus plantarum K3 (CGMCC NO.34845) according to the viable count ratio of (1.5 to 2.5): 1. The method comprises the following steps: chopping miscanthus sinensis, adjusting the water content, spraying a zymophyte solution prepared from the complex microbial inoculant and brown sugar, and carrying out vacuum sealing and anaerobic fermentation to obtain the miscanthus sinensis silage. The complex microbial inoculant utilizes rapid acid production of LW8 and later-stage stability of K3 to synergistically promote lactic acid fermentation, can rapidly reduce the pH value, significantly inhibit protein degradation (ammoniacal nitrogen / total nitrogen is less than or equal to 0.70%) and reduce the fiber content, and finally obtains miscanthus silage with stable fermentation quality and high nutritional value.

The invention relates to a fiber-based smokeless tobacco product and a preparation method thereof. The smokeless tobacco product is prepared from the following raw materials: plant fibers, a tobacco extract, a taste regulator, glycerol, starch, a gum base and a foaming agent, plants in the plant fibers comprise any one or a combination of at least two of sugarcane, corn, sorghum, bamboo reed, polygonum cuspidatum, miscanthus sinensis, poria cocos, celery or water spinach. According to the invention, a specific variety of plant fibers are applied to the preparation of the smokeless tobacco product, so that the product is endowed with specific taste and fragrance of related plants, and the effects of enriching fragrance, making the tobacco fragrance delicate and soft, reducing irritation and improving aftertaste can be achieved.

The invention belongs to the field of materials, and particularly relates to a light cement-based vegetation material, a preparation method and application thereof. The invention provides a specific light cement-based vegetation material, the cement-based vegetation mortar is mainly composed of cement, plant-based aggregate and water, and the plant-based aggregate comprises miscanthus powder and / or peach shells. According to the series of light cement-based vegetation materials, through the porous characteristic of the miscanthus powder and strict control over the mass ratio of the cement to the miscanthus powder, the product has the characteristics of high water absorption and water slow release.

The application discloses a salt-tolerant gene MsaH2A.W from Miscanthus sacchariflorus and application thereof, and belongs to the technical field of biotechnology.A new salt-tolerant gene MsaH2A.W is cloned and identified from Miscanthus sacchariflorus for the first time.The MsaH2A.W gene is transformed into plants to be overexpressed, so that the salt-tolerant ability of the transgenic plants can be significantly improved, the root growth of the transgenic plants under salt stress is promoted, and then the yield and quality of the transgenic plants are improved.Therefore, the MsaH2A.W gene has very important significance for improving the growth of plants under salt stress.

This invention belongs to the field of mine wasteland remediation technology, specifically disclosing a plant-microorganism synergistic method for mine wasteland remediation. The method includes the following steps: mixing organic waste, an alkaline amendment, and a first compound microbial agent with the topsoil of the mine wasteland, and conducting aerobic cultivation to obtain pretreated soil; tilling the soil, planting Miscanthus sinensis, and cultivating until the Miscanthus sinensis grows stably; planting ramie between the Miscanthus sinensis rows, spraying a second compound microbial agent on the soil surface between the rows, and harvesting the above-ground parts of the Miscanthus sinensis and ramie after 25-35 days to determine the metal accumulation; detecting the target metal content in the soil, and if the target is not reached, retaining the root system for multiple rounds of remediation. This method can effectively adsorb heavy metals in the soil, solving the problems of low remediation efficiency, long cycle, lack of resource consideration, and insufficient restoration of soil ecological function, providing an efficient, economical, and environmentally friendly solution for the sustainable ecological restoration of mine wastelands.

The invention discloses hermetia illucens fermentation liquor as well as a preparation method and application thereof. The hermetia illucens fermentation liquor is obtained by uniformly mixing hermetia illucens powder, wheat bran, enzymolysis miscanthus sinensis powder, sterile water, an aerobic leavening agent and papain, performing aerobic fermentation to obtain a hermetia illucens primary fermentation material, and then adding an anaerobic leavening agent for anaerobic fermentation. According to the invention, bacillus subtilis and aspergillus oryzae are introduced as aerobic leavening agents and cooperate with papain to ferment hermetia illucens, and enzymatic miscanthus powder is introduced to cooperate with an anaerobic leavening agent prepared by compounding bacillus amyloliquefaciens, lactobacillus plantarum and a plant composite extract; and the hermetia illucens fermentation liquor with high nutritional value and high quality is prepared, so that the hermetia illucens fermentation liquor has higher value and better effect in feed application.

The utility model relates to the technical field of triarrhena lutarioriparia picking, in particular to equipment suitable for rapidly picking triarrhena lutarioriparia, which comprises a rod body, a mounting plate is arranged in the rod body, a first air cylinder and a second air cylinder are arranged on the mounting plate, and a connecting seat is arranged at the output end of the first air cylinder. A cutting tool is arranged at the output end of the second air cylinder, a sliding plate and a swing arm are arranged on the portion, between the first air cylinder and the second air cylinder, of the mounting plate, the connecting base is connected with the sliding plate, a sliding groove is formed in the plate face of the sliding plate, a thrust block is arranged at the front end of the swing arm, and a sliding ball is arranged at the rear end of the swing arm. The sliding ball is arranged in the sliding groove and slides along the sliding groove. In the picking process, it is only needed to place the to-be-picked southern diels into the cutting notch, automatic and rapid picking can be achieved through front-back stretching and retracting of the cutting tool, manual work does not need to participate in cutting work, the working intensity can be lowered, and meanwhile a large amount of time can be saved.

Low moisture content at harvest is important since green biomass material is not suitable for many applications such as for combustion in power stations. It is unusual to find a Miscanthus variety that is both cold tolerant and / or vigorous but that dries down well in years with mild winters. A mild winter may reduce the amount of usable biomass that is obtained from Miscanthus plants, including from cold hardy varieties that will stay green and fail to dry down properly at the end of a season unless there is a heavy prolonged frost. To address this issue, the present description relates to the production of increased usable biomass yield that may be obtained at harvest from Miscanthus plants that are cold tolerant, vigorous, and which also have good dry down properties during mild winters. The present methods include plants and methods for regenerating plants from a cell, rhizome, or cutting of a Miscanthus sacchariflorus x Miscanthus sinensis cross, and then selecting one or more regenerated plants that produce increased usable biomass and / or increased carbon sequestration.