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A kind of culture method that can effectively reduce the rate of reverse mutation of wasabi polyploid plants

A technology of reverse mutation and culture method, which is applied in the field of cultivation to effectively reduce the reverse mutation rate of wasabi polyploid plants, and can solve the problems of plant polyploid induction failure and fast division speed, so as to ensure stability and reduce the reverse mutation rate Effect

Active Publication Date: 2016-04-06
广元市玺府生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is mainly due to the fact that the undoubled diploid cells divide faster than the induced polyploid cells, so the diploid cells may eventually "flood" the polyploid cells during the growth of chimeric plants, As a result, the induced polyploid plants revert to diploid, which eventually leads to the failure of plant polyploid induction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Select the wasabi seeds that were full that year, sterilize them with 0.01% peracetic acid for 10 minutes, then wash them with sterile water twice, and then store them at a low temperature of 3°C for 10 days;

[0021] (2) Put the wasabi seeds preserved at a low temperature on a filter paper soaked with a mixed solution of 5-aminouracil and propylene glycol for 10 hours at a culture temperature of 5°C. The mixed solution of 5-aminouracil and propylene glycol has a concentration of 0.5mmol.L -1 The 5-aminouracil solution and the concentration is 0.3mol.L -1 The propylene glycol solution is prepared at a volume ratio of 1:0.95;

[0022] (3) Wash the cultured horseradish seeds with sterile water twice, then place them at 12°C for recovery culture with sterile water for 5 hours, and then transfer them to a mixture of sulfadiazine and pentochlor. The solution was cultured on the filter paper for 3 hours, the culture temperature was 4°C, and the light intensity was 2000lx. The ...

Embodiment 2

[0027] (1) Select the wasabi seeds that were full that year, disinfect them with 0.04% peroxyacetic acid for 20 minutes, then wash them with sterile water 3 times, and then store them at a low temperature of 6°C for 12 days;

[0028] (2) Put the wasabi seeds preserved at a low temperature on a filter paper soaked with a mixed solution of 5-aminouracil and propylene glycol for 18 hours at a culture temperature of 8°C. The concentration of the mixed solution of 5-aminouracil and propylene glycol is 1.5mmol.L -1 The 5-aminouracil solution and the concentration is 0.5mol.L -1 The propylene glycol solution is prepared at a volume ratio of 1:1;

[0029] (3) Wash the cultured horseradish seeds with sterile water for 3 times, then place them at 15°C for recovery and culture with sterile water for 7 hours, and then transfer them to a mixed solution of sulfasulfame and pentochlor. Cultivate 7h on the filter paper, the culture temperature is 6 ℃, the light intensity is 2500lx, the concentrati...

Embodiment 3

[0034] (1) Select the wasabi seeds that were full that year, disinfect them with 0.07% peroxyacetic acid for 30 minutes, then wash them with sterile water 5 times, and then store them at a low temperature of 10°C for 15 days;

[0035] (2) Put the wasabi seeds preserved at low temperature on a filter paper soaked with a mixed solution of 5-aminouracil and propylene glycol for 24 hours at a temperature of 9°C. The mixed solution of 5-aminouracil and propylene glycol has a concentration of 2.5mmol.L -1 The 5-aminouracil solution and the concentration is 0.7mol.L -1 The propylene glycol solution is prepared at a volume ratio of 1:1.05;

[0036] (3) Wash the cultured horseradish seeds with sterile water for 5 times, then place them at 18°C ​​for recovery and culture with sterile water for 9 hours, and then transfer them to a mixed solution of sulfasulfame and pentochlor. Cultured on the filter paper for 10h, the culture temperature is 10℃, the light intensity is 3000lx, and the mixed so...

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Abstract

The invention discloses a culturing method capable of effectively reducing the reverse mutation rate of horseradish polyploid plants. The method comprises the following steps: disinfecting horseradish seeds and sequentially culturing by a mixed solution of 5-aminuracil and propylene glycol, a mixed solution of oryzalin and propyzamide, and a mixed solution of colchicine and fumiron. With the culturing method, the reverse mutation rate of polyploid seedlings cultured by obtained polyploid seeds can be effectively reduced; the culturing method is a new way for rapidly culturing good horseradish varieties.

Description

Technical field [0001] The invention relates to a tissue culture method of wasabi plants, in particular to a culture method that can effectively reduce the back mutation rate of wasabi polyploid plants. Background technique [0002] Wasabijaponica Matsum, also known as Wasabi, is a semi-shade plant of the cruciferous perennial herb. [0003] The propagation of wasabi plants mostly adopts ramet propagation and seed propagation. Ram propagation not only has the problem of serious degradation of varieties after multi-generation vegetative propagation, but also has the problems of low reproduction coefficient and serious germ-carrying in seedlings, which can easily lead to large-scale diseases. At present, wasabi resources are very scarce, far from being able to meet market demand, so it is necessary to cultivate high-quality new varieties of wasabi. Polyploid plants have the characteristics of huge organs, and because the cell chromosomes are doubled, the content of effective chemic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王跃华孙雁霞段茂华任三军陈传凤彭双严幸林梅英徐恩琴唐凤如宋超刘银花唐川
Owner 广元市玺府生物科技有限公司
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