The invention relates to the technical field of herbicide preparation, in particular to a herbicide for preventing and controlling broadleaf weeds in a cotton field and a preparation process of the herbicide. The herbicide comprises the following components in parts by weight: 35-45 parts of a mixed medicament formed by mixing metamitron and a pendimethalin suspending agent according to the weightratio of (3-7): (0.8-1.2), 8-10 parts of modified trifluralin, 5-8 parts of saflufenacil, 13-20 parts of a 48% flumioxazin suspending agent, 25-30 parts of a 30% oxadiargyl suspending agent, 20-30 parts of acetochlor, 3.5-4.2 parts of carbopol, 1.2-1.8 parts of a disintegrating agent, 1.6-2.2 parts of an adhesive, 1.4-2.0 parts of a wetting agent and 2.2-2.7 parts of a dispersing agent. The metamitron, the pendimethalin suspending agent, the acetochlor and the modified trifluralin are used as raw materials for preparing the herbicide, so that effective components in the herbicide are absorbedby hypocotyls of field broadleaf weeds, and meanwhile, cotyledons can well absorb the effective components in the herbicide, so that the weeding effect of the herbicide is improved; moreover, the modified trifluralin has good light stability, so that the drug effect of the trifluralin is prolonged.