Tissue culture and rapid propagation method of oriental cherries

A tissue culture, cherry blossom technology, applied in the fields of botanical equipment and methods, horticultural methods, horticulture, etc., can solve the problems of restricting the application of cherry blossoms, the rooting rate of the proliferation coefficient is less than 50%, the low survival rate, etc., and achieves good market application prospects. The effect of strong adaptability and high rooting rate

Inactive Publication Date: 2016-04-20
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the tissue culture and rapid propagation process of cherry blossoms in the prior art: the germination rate of explants is about 30-40%, the multiplication coefficient is 1-2.4, and the rooting rate is less than 50%. The lower survival rate seriously restricts the use of cherry blossoms in urban greening and gardens. Applications under construction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The steps of cherry blossom tissue culture and rapid propagation method are:

[0028] (1) Acquisition of cherry blossom stem segment explants:

[0029] After flowering in spring, take the young branches with terminal buds that grow normally, healthy and strong, and have no pests and diseases on the cherry blossom mother plant, cut them into branches of about 5cm, wash them in running water for 2 hours, and then immerse the materials on the ultra-clean workbench. Soak in 70% alcohol for 30s, rinse with sterile water for 3 times, then disinfect with 0.1% mercury liter for 3 minutes, rinse with sterile water for 3 times, repeat 0.1% mercury liter disinfection for 3 minutes, and rinse with sterile water for 3 times. Put the sterilized materials into a sterile petri dish, absorb excess water with absorbent paper, and cut the branches into stems with an axillary bud.

[0030] (2) induction culture of cherry blossom stem explants:

[0031] Cultivate the stem segment with an ...

Embodiment 2

[0039] The steps of cherry blossom tissue culture and rapid propagation method are:

[0040] (1) Acquisition of cherry blossom stem segment explants:

[0041] After flowering in spring, take the young branches with terminal buds that grow normally, healthy and strong, and have no pests and diseases on the cherry blossom mother plant, cut them into branches of about 3cm, rinse them in running water for 4 hours, and then immerse the materials on the ultra-clean workbench. Soak in 70% alcohol for 40s, rinse with sterile water 5 times, then disinfect with 0.1% mercury liter for 5 minutes, rinse with sterile water 5 times, repeat 0.1% mercury liter disinfection for 5 minutes, rinse with sterile water 5 times. Put the sterilized materials into a sterile petri dish, absorb excess water with absorbent paper, and cut the branches into stems with an axillary bud.

[0042] (2) induction culture of cherry blossom stem explants:

[0043] Culture the stem section with one axillary bud in ...

Embodiment 3

[0051] The steps of cherry blossom tissue culture and rapid propagation method are:

[0052] (1) Acquisition of cherry blossom stem segment explants:

[0053] After flowering in spring, take the young branches with terminal buds that grow normally, healthy and strong, and have no pests and diseases on the cherry blossom mother plant, cut them into branches of about 4cm, wash them in running water for 3 hours, and then immerse the materials on the ultra-clean workbench. Soak in 70% alcohol for 35s, rinse with sterile water 4 times, then disinfect with 0.1% mercury liter for 4 minutes, rinse with sterile water 4 times, repeat 0.1% mercury liter disinfection for 4 minutes, rinse with sterile water 4 times. Put the sterilized materials into a sterile petri dish, absorb excess water with absorbent paper, and cut the branches into stems with an axillary bud.

[0054] (2) induction culture of cherry blossom stem explants:

[0055] Culture the stem section with one axillary bud in t...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method of oriental cherries. The tissue culture and rapid propagation method of the oriental cherries comprises the following steps: obtaining oriental cherry stem explants, carrying out induced culture on the oriental cherry stem explants, carrying out multiplication culture on oriental cherry cluster buds, carrying out rooting culture of the oriental cherries, carrying out seedling hardening on oriental cherry tissue culture seedlings and transplanting the oriental cherry tissue culture seedlings. Culture media prepared by the invention are used in induced culture, multiplication culture and rooting culture processes, and obtained seedlings easily take root, are healthy and strong and have resistance and strong disease and pest resistance; the seedlings are ordered and consistent, have high quality and are relatively easy to cultivate and manage. The tissue culture seedlings obtained by the invention have a strong adaptive capacity on a hot and humid environment and straight stems; and the quality is improved by 1-2 grades and the value is increased by 20%-40%.

Description

technical field [0001] The invention relates to a plant cultivation method, in particular to a cherry blossom tissue culture and rapid propagation method. Background technique [0002] Cherry blossoms generally refer to the collective name for the flowers of plants of the genus Cerasus in the subfamily Prunoidea of ​​the Rosaceae family. Cherry blossoms are woody plants. The traditional propagation methods mainly use cuttings, grafting and other propagation methods, but these propagation methods require a large number of mother plants on the one hand, and on the other hand, they also need to spend a lot of manpower, material resources, financial resources, time, etc., and the reproduction coefficient is low. , the seedlings are slow. With the wide application of cherry blossoms in urban and township landscaping construction, large-scale seedlings are needed. Therefore, traditional breeding methods are far from being able to meet the needs of production. [0003] With the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 蒋细旺张灵灵
Owner JIANGHAN UNIVERSITY
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