Calla lily germplasm resources in vitro conservation method

Abstract

The invention discloses an in vitro conservation method of a color calla idioplasm resource, pertaining to the vegetable idioplasm resource conservation and tissue cultivation technical field. The method comprises such steps as preparation of a culture medium, disinfection of an explant and inoculation and induction culture, multiplication culture, root culture, a corm intumescence culture, idioplasm conservation culture, restore culture, and the detection and utilization of genetic stability, etc. The method adopts the meristem tissue of a 0.5mm stem tip of the color calla as the explant, a large quantity of elements in the culture medium are reduced and mannitol and penicillin are added, and a protocorm seedling is conserved at 10 to 15 DEG C under the surroundings of light intensity of 1000Lx for 10h / d, and after 15 months, the contamination of Erwinia is eliminated, the survival rate reaches 100 percent; the growth and multiplication are still normal without genetic variation. The method of the invention is characterized by low cost and simple operation etc., and can be popularized and applied in the idioplasm conservation field of the color calla.

Description

technical field [0001] The invention relates to the technical field of plant germplasm resource preservation and tissue culture, in particular to a method for in vitro preservation of colored calla lily germplasm resources. Background technique [0002] Colored calla lily (Zantedeschia hybrida) is a perennial monocot herbaceous bulbous flower of the genus Zantedeschia in the family Araceae. It is elegant in shape and bright in color. channel. In recent years, it has gradually been favored by Chinese consumers, and the planting area is constantly expanding. Most of the colored calla lilies are cultivated as annuals, and they are propagated asexually through bulbs. The reproduction and preservation of colored calla lily germplasm resources also mainly rely on bulbs as propagation materials for field planting every year, which consumes a lot of manpower, material and financial resources, and the colored calla lily bulbs are easily infected with bacterial soft rot. After the ...

AI Technical Summary

Problems solved by technology

But the simple use of the above preservation methods is not suitable for colored calla lilies. First of all, the bulbs of colored calla lilies are easy to carry Erwinia (can cause bacterial soft rot), especially the cut pieces with bud eyes are usually used as explants , during the rapid propagation process, with the increase of the number of culture generations, the endophytic bacteria also multiply and spread in large numbers, resulting in the inability to continue the rapid propagation of tissue culture; secondly, the Erwinia is endophytic bacteria, if it is disinfected by conventional mercury chloride surface Cannot reach ideal control effect, this is also a bottle stem problem to the long-term ex vivo preservation of colored calla lily; Again, must carry out frequent subculture (about every month) to colored calla lily germplasm resource in conventional tissue culture preservation In order to better maintain its species; in addition, colored calla lily bulbs often carry a large number of viruses in addition to Erwinia, which is also a problem that must be considered in tissue culture and in vitro preservation.