Calla lily germplasm resources in vitro conservation method

A technology of in vitro preservation and calla lily, applied in the fields of plant preservation, botanical equipment and methods, application, etc., can solve problems such as failure to achieve control effects and inability to continue, and achieve reduced workload, easy operation, and overcome pollution. effect of the problem

CN101185433AInactive Publication Date: 2008-05-28ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2008-05-28
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses an in vitro conservation method of a color calla idioplasm resource, pertaining to the vegetable idioplasm resource conservation and tissue cultivation technical field. The method comprises such steps as preparation of a culture medium, disinfection of an explant and inoculation and induction culture, multiplication culture, root culture, a corm intumescence culture, idioplasm conservation culture, restore culture, and the detection and utilization of genetic stability, etc. The method adopts the meristem tissue of a 0.5mm stem tip of the color calla as the explant, a large quantity of elements in the culture medium are reduced and mannitol and penicillin are added, and a protocorm seedling is conserved at 10 to 15 DEG C under the surroundings of light intensity of 1000Lx for 10h / d, and after 15 months, the contamination of Erwinia is eliminated, the survival rate reaches 100 percent; the growth and multiplication are still normal without genetic variation. The method of the invention is characterized by low cost and simple operation etc., and can be popularized and applied in the idioplasm conservation field of the color calla.
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Description

technical field

[0001] The invention relates to the technical field of plant germplasm resource preservation and tissue culture, in particular to a method for in vitro preservation of colored calla lily germplasm resources. Background technique

[0002] Colored calla lily (Zantedeschia hybrida) is a perennial monocot herbaceous bulbous flower of the genus Zantedeschia in the family Araceae. It is elegant in shape and bright in color. channel. In recent years, it has gradually been favored by Chinese consumers, and the planting area is constantly expanding. Most of the colored calla lilies are cultivated as annuals, and they are propagated asexually through bulbs. The reproduction and preservation of colored calla lily germplasm resources also mainly rely on bulbs as propagation materials for field planting every year, which consumes a lot of manpower, material and financial resources, and the colored calla lily bulbs are easily infected with bacterial soft rot. After the ...

Examples

Embodiment 1

[0028] Embodiment 1: (coloured calla lily germplasm resources in vitro preservation method 1)

[0029] (1) Preparation of culture medium:

[0030] Basic Medium:

[0031] 1) The induction and proliferation medium are 30g / L sucrose and MS of agar 7g / L as the basic medium;

[0032] 2) rooting medium is basic medium with sucrose 30g / L, 1 / 2MS of agar 7g / L;

[0033] 3) The test tube corm expansion medium uses sucrose 40-50g / L, agar 6g / L MS as the basic medium;

[0034] 4) The germplasm preservation medium is 1 / 2MS with 20g / L sucrose and 8g / L agar;

[0035] The pH of the above basic medium is 5.6-5.8;

[0036] Among them, 1 / 2MS basic medium is Murashige-Skoog (MS) medium with macroelements halved;

[0037] (2) Disinfection, inoculation and induction culture of explants: Select a healthy, full-colored calla lily cue ball, wash off the soil and the outermost layer of brown skin on the cue ball with tap water, rinse it under running water for about 20 minutes, and dissect it with ...

Embodiment 2

[0044] Embodiment 2: (coloured calla lily germplasm resources in vitro preservation method 2)

[0045] In this example, the induction medium is M1: MS+6-BA1.5mg / L+IBA0.3mg / L+penicillin 50mg / L, the culture conditions are 24°C, the light intensity is 3000Lx, and the light is 12h / d;

[0046] Proliferation medium is M2: MS+6-BA0.8mg / L+IBA0.3mg / L+penicillin 100mg / L, culture conditions are 25°C, light intensity 2500Lx, and light 12h / d for one month;

[0047] The rooting medium is M3: 1 / 2MS+6-BA0.4mg / L+NAA0.5mg / L+Ac1g / L, the culture condition is 23℃, the light intensity is 3000Lx, and the light is 12h / d for one month;

[0048] The corm expansion culture is M4: MS+6-BA2.0mg / L+NAA0.5mg / L+Ac1g / L, the culture conditions are 22°C during the light period, 13°C during the dark time, 14h / d light time, light intensity 2500Lx;

[0049] The germplasm preservation medium is M5: 1 / 2MS+6-BA0.2mg / L+NAA0.3mg / L+mannitol 15.0g / L+penicillin 100mg / L, the culture condition is 10℃, the light is 10h / d, t...

Embodiment 3

[0051] Embodiment 3: (coloured calla lily germplasm resources in vitro preservation method 3)

[0052] In this example, the induction medium is M1: MS+6-BA1.0mg / L+IBA0.5mg / L+penicillin 50mg / L; the culture conditions are 22°C, light intensity 2000Lx, light 12h / d;

[0053] Proliferation medium is M2: MS+6-BA0.5mg / L+IBA0.15mg / L+penicillin 100mg / L, culture conditions are 23°C, light intensity 2000Lx, and light 12h / d for one month;

[0054] The rooting medium is M3: 1 / 2MS+6-BA0.3mg / L+NAA0.5mg / L+Ac1g / L, the culture conditions are 26°C, the light intensity is 2500Lx, and the light is 12h / d for one month;

[0055] The corm expansion culture is M4: MS+6-BA1.5mg / L+NAA0.5mg / L+Ac1g / L, the culture conditions are 24°C during the light period, 13°C during the dark period, and 16h / d, light intensity 2000Lx;

[0056] Germplasm preservation medium is M5: 1 / 2MS+6-BA0.3mg / L+NAA0.4mg / L+mannitol 18.0g / L+penicillin 100mg / L, culture condition is 13℃, light is 10h / d, light intensity 1000Lx;

[00...