The invention discloses a low-
oxygen affinity deoxidized
hemoglobin spray and application thereof. The invention creatively discovers that the
oxygen concentration on the surface of a wound is obviously higher than that of pure deoxidized
hemoglobin and high-
oxygen-affinity deoxidized
hemoglobin with higher
oxygen carrying capacity after the low-oxygen-affinity deoxidized hemoglobin with higher oxygen release capacity is used; it is proved that the low-
oxygen affinity deoxidized hemoglobin is more beneficial to improvement of the oxygen concentration of the
wound surface. According to the specific embodiment of the invention, the RSR-13 is combined with the deoxidized hemoglobin, the conformation of the deoxidized hemoglobin is changed, the
oxygen affinity of the deoxidized hemoglobin is reduced, the oxygen release of the deoxidized hemoglobin in the hypoxia tissue is effectively enhanced, the
drug has no obvious
toxicity to
skin cells, the effectiveness and safety of the
drug in
skin wound treatment are improved, and the application prospect is wide. The method is suitable for treating multiple types of wound surfaces, and has wide application value and market prospect.