Instrument-free multi-analyte diagnostic strip system for rapid detection of bovine mastitis in milk

The multi-analyte diagnostic strip system addresses the limitations of existing mastitis detection by using dry reagents to assess multiple biomarkers in raw milk, ensuring rapid and accurate herd management without instruments, enhancing decision-making and reducing antibiotic misuse.

WO2026133353A1 Publication Date: 2026-06-25NAIK ROSHAN RATNAKAR +1
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Patent Information

Application Number
PCT/IN2025/052061
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-12-16
Filing Date
2025-12-15
Publication Date
2026-06-25

AI Technical Summary

Technical Problem

Current mastitis detection methods in dairy farming are inadequate for rapid, accurate, and cost-effective on-farm decision-making, as they suffer from operator variability, logistical constraints, and limited diagnostic scope, particularly in detecting multiple biomarkers relevant to mastitis.

Method used

An instrument-free, multi-analyte diagnostic strip system that uses dry reagent chemistries to detect multiple mastitis-associated biomarkers (pH, leukocyte esterase, haemoglobin, and nitrite) in raw milk, with a laminated structure and packaging system to ensure stability and standardization, enabling results within minutes without laboratory infrastructure.

Benefits of technology

Provides rapid, reliable, and comprehensive mastitis assessment, supporting timely treatment decisions and reducing unnecessary antibiotic use by integrating multiple indicators into a single, portable, and cost-effective test.

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Abstract

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.
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