The invention relates to cultivation soil, in particular to seedling-cultivating substrate soil for micro agriculture. The seedling-cultivating substrate soil is formed by mixing a substrate with rural soil or clay and comprises the following components in weight percent: 25% to 45% of the substrate and 55% to 75% of the rural soil or clay; the substrate comprises 60% to 80% of organic matters in weight percent and includes 1% to 3% of nitrogen, 1.5% to 3.5% phosphorus, 2% to 4% of potassium, and 0.02% to 0.8% of trace elements including magnesium, calcium, iron, manganese and boron; the pH value of the substrate is 6.5 to 6.8; the organic matters comprise 1 to 4 parts of bamboo vinegar liquid, 1 to 3 parts of starch-acrylic acid graft copolymer, 15 to 40 parts of a straw decomposition agent, 15 to 40 parts of fermented human and animal excreta, 10 to 18 parts of edible fungus residue, 3 to 6 parts of waste bamboo, 2 to 5 parts of plant-charcoal powder, 5 to 10 parts of silt slag, 4 to 6 parts of bone meal and 4 to 9 parts of soybean meal. According to the invention, specific components and proportion are adopted to prepare the organic matters, and the organic matters are combined with inorganic matters to form the acidulous substrate, so that the seedling-cultivating substrate soil is high in nutritional ingredient and appropriate in proportion of trace elements, has an excellent odor removing effect, and avoids undesirable odors produced during planting of vegetables and other plants for urban families.