Preparation and application of antimicrobial peptide fusion cytokine campils co-expression biological preparation

Abstract

The invention discloses the preparation and application of an antimicrobial peptide fusion cytokine CAMPILs co-expression biological preparation. The antimicrobial peptide fusion cytokine CAMPILs provided by the present invention is as follows A1), A2) or A3): A1) the amino acid sequence is the protein of sequence 1 in the sequence listing; A2) the amino acid sequence shown in sequence 1 in the sequence listing is passed through a or several amino acid residue substitutions and / or deletions and / or additions and proteins with the same function; A3) A fusion protein obtained by linking tags at the N-terminal or / and C-terminal of A1) or A2). Experiments have proved that CAMPILs of the present invention can promote the proliferation of lymphocytes, red blood cells and white blood cells, inhibit the growth of pathogenic microorganisms, promote the secretion of non-specific antibodies (IgG, IgG1, IgG2a) and disease-specific antibodies, and improve the immunity and survival of animals. Rate.

Description

technical field [0001] The invention relates to the preparation and application of an antimicrobial peptide fusion cytokine CAMPILs co-expression biological preparation in the field of biotechnology. Background technique [0002] The aquaculture industry (animal husbandry and aquatic products) is an important part of large-scale agriculture. It is one of the important foundations for my country's agricultural modernization, rural economic development, and social stability. The aquaculture industry in advanced countries in Europe and the United States is very developed, accounting for 50% of large-scale agriculture. % above the specific gravity. It provides human beings with meat, milk, eggs, fur and other high-grade foods and important textile raw materials. Animal products are the main source of high-quality protein, minerals, vitamins and other nutrients for people. Their safety is directly related to human health. In recent years, vicious events such as poisoning and dise...

AI Technical Summary

Problems solved by technology

The abuse of feed antibiotic additives not only increases the cost of feeding, but also leads to a significant increase in the drug resistance and pathogenicity of pathogenic microorganisms, and the health of livestock and poultry bodies is weakened due to the side effects of drugs, and the immunity and disease resistance are significantly reduced

In such a vicious cycle, various severe infectious diseases of livestock and poultry frequently occur, making it difficult to control and treat

On the other hand, drug residues and the increasingly serious food safety problems of livestock and poultry products not only directly threaten the health of human beings themselves, but also hinder the development of the breeding industry.

[0006] The addition of antibiotics to animal feed, long-term or inappropriate use will not only induce drug resistance in bacteria, but also cause drug residues in animal products, causing serious food safety hazards, damaging human health and inducing the failure of drugs used to treat antibiotics

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