Method for detecting embryo health by using blastocyst culture solution, and product

Abstract

The invention provides a method for detecting embryo health by using a blastocyst culture solution, and a product. In particular, the invention provides the method for detecting embryo health by usinga blastocyst culture solution in vitro. The method is characterized by comprising the following steps: (a) providing a first blastocyst culture system, wherein the first blastocyst culture system contains blastocysts with culture days of D5-D6 in vitro; (b) transferring the blastocysts to a second blastocyst culture system containing a fresh blastocyst culture solution for liquid exchange cultureso as to obtain a cultured blastocyst culture solution, wherein the liquid exchange culture time is T1; (c) taking out the cultured blastocyst culture solution to obtain a cell-free cultured blastocyst culture solution, namely a detection sample; and (d) performing gene detection on the detection sample to identify the health condition of the blastocysts. The method provided by the invention canremove the risk of pollution interference of superfluous sperms and maternal granulosa cells in the IVF and ICSI technical processes, and can accurately identify the health condition of embryos.

Description

technical field [0001] The invention relates to the fields of biomedicine and molecular cell biology, in particular to a method and a product for detecting the health status of embryos using blastocyst culture fluid. Background technique [0002] Preimplantation Genetic Screening (Preimplantation Genetic Screen, PGS) detects the chromosomal status of in vitro cultured embryos to screen normal chromosomal embryos for placement in the mother's uterus, thereby increasing the pregnancy success rate to about 60%. The biological samples required for various tests are one to several cells collected from embryos cultured in vitro, commonly known as biopsy, and the detection of this small number of cells reflects whether the chromosomes of the entire embryo are normal. Theoretically speaking, the chromosomal state of the aspirated cells is consistent with other cells in the embryo, and testing these cells can determine whether the chromosomal state of the embryo is normal. It is gen...

AI Technical Summary

Problems solved by technology

For example, Kuznyetsov-Evaluation of a novel non-invasive preimplantation genetic screening approach, one of which uses fresh embryos, and also replaces the medium on D4 to D5 to collect blastocoel fluid after laser shrinkage, which can only be used for ICSI samples and cannot For IVF samples

In these literatures, the in vitro incubation time was advanced to D4 and collected on D5 / D6. On the one hand, the in vitro incubation time of the test samples was 12-36 hours. If the incubation time is too long, it is still inevitably affected by external interference, which makes the proportion of embryo-derived DNA The accuracy of embryo results has not been significantly improved, and the false negative rate is still about 15%, and it is impossible to target ICSI samples and IVF samples at the same time; Embryo development will cause unpredictable effects. D4 embryos usually develop into morula stage embryos, which is a critical period for embryos to develop into blastocysts. The next stage of morula is the blastocyst stage, and the blastocyst rate is a key indicator of embryo development. In vitro operations such as transplantation or freezing can only be considered after blastocysts are formed into cysts. Therefore, in vitro operations such as changing the culture medium for embryos at the D4 mulberry stage are usually not performed clinically, so as not to affect the cyst formation rate.

[0006] However, the current detection method, due to the inevitable interference of external sources, especially maternal interference, leads to a high false positive rate and cannot target both ICSI samples and IVF samples

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