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Method for culturing in-vitro fertilized embryo of mammal

A technology of in vitro fertilization and embryo culture, applied in agriculture-animal husbandry and veterinary reproduction, the application of related culture medium in in vitro fertilization embryo culture, in the field of in vitro fertilization embryo culture, can solve the high cost of in vitro fertilization embryo production, limit the wide application and efficiency low level problem

Inactive Publication Date: 2019-06-28
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although in vitro fertilization technology can be successfully applied to many mammals, the high cost and low efficiency of in vitro fertilized embryo production due to the low blastocyst rate of in vitro fertilization limit the wide application of this technology in rapid multiplication practice

Method used

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  • Method for culturing in-vitro fertilized embryo of mammal

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Embodiment Construction

[0055] The present invention can be further described by the following examples, however, the scope of the present invention is not limited to the following examples. Those skilled in the art can understand that various changes and modifications can be made in the present invention without departing from the spirit and scope of the present invention. The present invention provides general and / or specific descriptions of the materials and test methods used in the tests. While many of the materials and methods of manipulation which are employed for the purposes of the invention are well known in the art, the invention has been described here in as much detail as possible. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0056] In the specific test of the present invention, if not otherwise specified, the relevant reagents used ...

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Abstract

The invention relates to a method for culturing an in-vitro fertilized embryo of a mammal. The method comprises the following steps of collection and in-vitro maturation of an oocyte; in-vitro fertilization; in-vitro culture and preservation of the embryo. During in-vitro fertilization, mature COCs are cultured in a fertilization culture solution. During in-vitro culture of the embryo, granulosa cells around the embryo are removed by an egg stripping needle, the embryo is cultured in an embryo culture liquid, and the available embryo after culture is completed is cryopreserved in a freezing liquid with liquid nitrogen. The method has excellent technical effects described in the description.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and relates to a technology for agriculture-animal husbandry and veterinary reproduction, in particular to a method for culturing in vitro fertilized embryos. In addition, the present invention also relates to related culture fluid for culturing in vitro fertilized embryos in vitro fertilized embryos applications in cultivation. Further, the present invention also relates to a method for culturing in vitro fertilized embryos using the relevant culture medium for in vitro fertilized embryo culture. In particular, the method for culturing embryos through in vitro fertilization of the present invention has excellent technical effects. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) or (external fertilization) refers to the technology in which mammalian sperm and eggs complete the fertilization process in an artificially controlled environment outside the ...

Claims

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Application Information

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IPC IPC(8): C12N5/073
Inventor 王珊珊
Owner NANJING DRUM TOWER HOSPITAL
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