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Goat circular RNA, and identification method and functional application thereof

An identification method and a circular technology, applied in the field of genetic engineering, can solve problems such as limited research, achieve good application prospects, and promote the effect of granulosa cell proliferation.

Active Publication Date: 2019-04-12
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0004] There are many research papers on the mechanism of follicle development, and a lot of important conclusions have been obtained, but the research on the regulation of follicle development by non-coding RNAs, especially circRNAs, is still very limited.

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  • Goat circular RNA, and identification method and functional application thereof
  • Goat circular RNA, and identification method and functional application thereof
  • Goat circular RNA, and identification method and functional application thereof

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Embodiment Construction

[0029] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the examples, which are only used to explain the present invention, and do not constitute a limitation to the protection scope of the present invention.

[0030] according to figure 1 , 2 , 3, 4, 5, and 6, the present embodiment proposes a goat circular RNA, which is characterized in that: it includes goat circular RNA circ_ZCCHC24, and the nucleotide sequence of the goat circular RNA circ_ZCCHC24 is as SEQ ID NO: 1.

[0031] The primers used to detect the goat circular RNA circ_ZCCHC24 include an upstream primer (PF1) and a downstream primer (PR1), the nucleotide sequence of the upstream primer (PF1) is shown in SEQ ID NO: 2, and the downstream primer The nucleotide sequence of (PR1) is shown in SEQ ID NO:3, and the nucleotide sequence of the linker sequence of the goat circular RNA circ_ZCCHC24 is shown in SEQ ID NO:4.

[0032] A ...

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Abstract

The invention discloses a goat circular RNA as well as an identification method and functional application thereof. Nucleotide sequences of primers used for detecting the goat circular RNA circ_ZCCHC24 are respectively shown in the SEQ ID NO: 2 and the SEQ ID NO: 3. An over-expression vector constructed by an over-expression vector plasmid construction method of the goat circular RNA circ_ZCCHC24contains a circular RNA expression vector pCD2.1-ciR as well as full-length sequence information of the goat circular RNA circ_ZCCHC24, and is capable of promoting proliferation of goat follicular granulosa cells; and thus, new ideas for further understanding genetic mechanisms of goat follicular granulosa cell development are provided by the goat circular RNA circ_ZCCHC24. Therefore, the goat circular RNA circ_ZCCHC24 is of great significance for studying the genetic essence of goat reproductive traits and breeding high-breed-rate goat breeds.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a goat circular RNA and its identification method and functional application. Background technique [0002] Circular RNAs (circular RNAs, circRNAs) are a new type of endogenous non-coding RNAs, which are 5' of exons and / or introns during the splicing process of mRNA precursors (pre-mRNA). The end and the 3' end connect the 3' and 5' phospholipid bonds in the form of back splicing, and finally form a covalently closed circular RNA molecule. According to their composition, circRNAs are divided into exonic circRNAs (exonic circRNAs), intronic circRNAs (ciRNAs) and exon-intron circRNAs (EIciRNAs). CircRNA regulates the expression of the source gene (Host gene) in the nucleus, and plays the role of ceRNA (competing endogenous RNAs, endogenous competing RNA) in the cytoplasm. Following miRNA and lncRNA, circRNA has become a new hotspot in the field of non-coding RNA resea...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/113
CPCC12Q1/6888C12Q2600/178C12Q2600/124
Inventor 陶虎陈明新刘洋熊琪李晓锋索效军张年杨前平田宏张鹤山熊军波张凤
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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