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Iron-reduced tuftedmonas and its use

A technology of Comamonas and microbial strains, applied in the direction of bacteria, etc., can solve the problems of inability to use organic matter, unfavorable large-scale production and practical application, and high environmental requirements

Inactive Publication Date: 2007-11-14
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These strains still have the following defects: 1) Most strains can only survive in a strict anaerobic environment, which is not conducive to large-scale production and practical application; 2) The electron donor utilization spectrum of the strains is narrow, and only acetic acid, lactic acid, etc. Small-molecular-weight organic matter is used as an electron donor, and organic matter with a larger molecular weight such as glucose and sucrose cannot be used, and the environmental requirements for practical applications are relatively high.
So far no Comamonas species with humus-reducing or Fe(III)-reducing activity have been found

Method used

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  • Iron-reduced tuftedmonas and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Separation, purification of bacterial classification

[0023] Take 5g ancient forest soil sample in 100mL liquid medium, the medium is composed of (g / L): AQDS (anthraquinone-2,6-disulfonic acid) 0.0412g, sodium acetate 1.36g, NaHCO 3 2.5g, NH 4 Cl 0.25g, NaH 2 PO 4 2H 2 O 0.678g, KCl 0.1g, vitamin solution 10.0mL (each liter of vitamin solution contains: biotin 2.0mg, folic acid 2.0mg, vitamin B 6 10.0mg, riboflavin 5.0mg, vitamin B 1 50mg, Niacin 5.0mg, Pantothenic Acid 5.0mg, Vitamin B 12 0.1mg, lipoic acid 5.0mg), trace element solution 10.0mL (each liter of trace element solution contains: nitrilotriacetic acid 1.5g, MgSO 4 ·7H 2 O 3.0g, MnSO 4 ·H 2 O 0.5g, NaCl 1.0g, FeSO 4 ·7H 2 O 0.1g, CoCl 2 ·6H 2 O 0.1g, CaCl 2 0.1g, ZnSO 4 ·7H 2 O 0.1g, CuSO 4 ·5H 2 O 0.01g, AlKSO 4 12H 2O 0.01g, H 3 BO 3 0.01g, Na 2 m 0 o 4 0.01g, NiCl 2 ·6H 2 O 0.024g), pH 5.0-5.5. Before inoculation, the culture medium is filled with high-purity ...

Embodiment 2

[0024] Embodiment 2: the identification of strain

[0025] (1) Determination of physiological and biochemical properties:

[0026] Table 1. Physiological and biochemical characteristics of CY01 strain

[0027] Test items

result

Test items

result

s

cell shape

cell size

Chromogenic

athleticism

Colony morphology

Colony size

gas production

Colony color (24h)

Colony color (48h)

oxidase reaction

Anaerobic conditions

Reduced ferrihydrite (anaerobic condition)

Reduced ferric citrate (anaerobic conditions)

Reduced ferric hydroxide (anaerobic conditions)

Reduced goethite (anaerobic conditions)

sucrose

Maltose

Negative

long rod

1.2~1.5×0.3~0.4μm

-

+

round

1~2mm

-

light pink

yellow

+

facultative anaerobic

+

+

+

+

-

-

D-fructose

L-Trehalose

D-galactose

D-Mannitol

D-Mannose

methyl pyruvate

Monomet...

Embodiment 3

[0031] Example 3: Identification of reducing activity

[0032] The electron donor is glucose and the electron acceptor is ferrihydrite

[0033] Medium formula: 10g of hydrous iron ore per liter of deionized water, NaHCO 3 2.5g, NH 4 Cl0.25g, NaH 2 PO 4 2H 2 O 0.678g, KCl 0.1g, glucose 0.9008g, each 10.0mL of vitamin solution and trace element solution (the formula of vitamin solution and trace element solution is the same as in Example 1), pH 5.0~5.5; When the glucose is separated from other components, the glucose and other components are mixed after sterilization, and then filled with high-purity mixed gas (N 2 / CO 2 =80 / 20)≥1 hour, inoculate the C. ferrireducens CY01 bacterial suspension to make the number of bacteria reach 10 8 / mL, static anaerobic culture at 30°C, and a control without bacteria was set at the same time. To observe the color change of the culture solution, take 4 mL of the culture solution in 16 mL of 0.5M HCl solution (M represents the number of...

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Abstract

This invention opens a hair iron reduction Cong Aeromonas and its applications. After a lot of inventors experimental study, and selected a hair iron reduction Aeromonas Cong, a reduction of humus and iron reduction activity. This reduction of citric acid to iron, ferric hydroxide, water iron ore, goethite three-valent iron; can restore humus and humus model of AQDS (anthraquinone 2 ,6 - disulfonic acid). Iron reducing gross Cong Aeromonas facultative anaerobic, the electronic spectrum for the broader use of this bacterium in the anaerobic oxidation conditions to the electronic donor: glycerol, glucose and sucrose.

Description

technical field [0001] The invention relates to the technical field of new strain screening, in particular to Comamonas iron-reducing bacteria and its application. Background technique [0002] Fe(III) and humus are two components that are abundantly present in soil. Fe(III) mainly exists in the form of insoluble iron oxide, with an average content of about 5.6% in the earth's crust, and is the electron acceptor with the highest natural abundance in anaerobic soils. Humus is a quinone-rich, heterogeneous natural organic mixture. It is the main body of soil organic matter, accounting for about 60-80% of the total organic carbon. It plays an important role in soil fertility, sustainable agricultural development, and environmental protection. effect. [0003] Humus reduction, also known as humus respiration, refers to the process in which microorganisms transfer electrons to the humus in the soil through respiration, reduce the oxidized humus to the reduced humus, and the bac...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 周顺桂武春媛王弋博李芳柏
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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