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Anaerobic cellulose-degrading methane producing composite bacterium

A technology for degrading cellulose and producing methane, applied to bacteria, microorganisms, waste fuels, etc., can solve the problems of single enzyme production, difficulty in degrading lignocellulose, and unrecoverable culture medium, so as to increase methane content, speed up fermentation start, The effect of fast and efficient hydrolysis

Inactive Publication Date: 2009-07-08
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the pure culture has the following disadvantages: the single enzyme production, cellobiose and glucose, the decomposition products of cellulose, can inhibit the activity of cellulase and the synthesis of cellulase, and the accumulation of volatile acid will easily reduce the pH of the culture solution to It is too low to restore the conditions suitable for culture growth, so that the conversion efficiency of cellulosic substances is low; and without pretreatment and / or sterilization, the use of pure culture and pure enzymes to degrade lignocellulose has certain degree of difficulty
Although these two natural composite strains both have strong cellulose degradation ability and methane production ability, the strains contained in this natural fermented bacterial agent are assembled disorderly during the natural passive fermentation process. The multi-bacteria combination cannot grasp the composition of its strains, and the use effect is difficult to control; moreover, this natural complex strain passively follows the law of natural circulation, and its decomposition effect is difficult to maintain stability

Method used

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  • Anaerobic cellulose-degrading methane producing composite bacterium
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  • Anaerobic cellulose-degrading methane producing composite bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] An anaerobic cellulose-degrading methanogenic complex bacteria, composed of the following 13 strains, the cell number content of the 13 strains in the complex bacteria: Cytophaga fermentans accounted for 10%, Clostridium papyrosolvens accounted for 25%, Desulfovibrio vulgaris accounted for 1%, Butyrivibriofibrisolvens accounted for 1% 5%, Acetobacterium woodii 5%, Cellulomonas flavigena 1%, Clostridium termitidis 1%, Clostridium cellobioparum 2.5%, Syntrophomonas Wolfei 5%, Methanosarcina barkeri 19%, Methanobrevibacter arboriphilicus 15%, Methanosphaera stadtmaniae 8% vannielii accounted for 2.5%.

[0038] The composite bacteria with the above composition are inserted into the cellulose liquid culture medium with rice straw as the carbon source, sealed and cultured statically. See the table below for the straw degradation effect and the gas concentration results produced after cultivating for 15 days and 30 days:

[0039]

Embodiment 2

[0041] An anaerobic cellulose-degrading methanogenic complex bacteria, composed of the following 13 strains, the cell number content of the 13 strains in the complex bacteria: Cytophaga fermentans accounted for 7.5%, Clostridium papyrosolvens accounted for 35%, Desulfovibrio vulgaris accounted for 3%, Butyrivibriofibrisolvens accounted for 3% 4%, Acetobacterium woodii 5%, Cellulomonas flavigena 5%, Clostridium termitidis 3%, Clostridium cellobioparum 5%, Syntrophomonas Wolfei 5%, Methanosarcina barkeri 10%, Methanobrevibacter arboriphilicus 10%, Methanosphaera stadtmaniae 5%, vannielii accounted for 2.5%.

[0042] The composite bacteria with the above composition are inserted into the cellulose liquid culture medium with rice straw as the carbon source, sealed and cultured statically. See the table below for the straw degradation effect and the gas concentration results produced after cultivating for 15 days and 30 days:

[0043]

Embodiment 3

[0045] An anaerobic cellulose-degrading methanogenic complex bacteria, composed of the following 13 strains, the cell number content of the 13 strains in the complex bacteria: Cytophaga fermentans accounted for 6%, Clostridium papyrosolvens accounted for 25%, Desulfovibrio vulgaris accounted for 2%, Butyrivibriofibrisolvens 4%, Acetobacterium woodii 10%, Cellulomonas flavigena 5%, Clostridium termitidis 3%, Clostridium cellobioparum 3%, Syntrophomonas Wolfei 10%, Methanosarcina barkeri 13%, Methanobrevibacter arboriphilicus 10%, Methanosphaera stadtvanethanielcus 4% 5%.

[0046] The composite bacteria with the above composition are inserted into the cellulose liquid culture medium with rice straw as the carbon source, sealed and cultured statically. See the table below for the straw degradation effect and the gas concentration results produced after cultivating for 15 days and 30 days:

[0047]

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Abstract

The invention discloses anaerobic degradation cellulose methane composite bacteria, which is composed of 13 strains that respectively belong to facultative anaerobic and strictly anaerobic fermentative bacteria, hydrogen-production and acetic acid-production bacteria and methane-producing bacteria. The composite bacteria can not only effectively perform anaerobic degradation to the cellulose, but also can use the degradation production of the cellulose to produce methane, which can be widely used in straw biogas fermentation, compost processing, and the like conversion processes.

Description

technical field [0001] The invention relates to the technical field of cellulose degradation and energy utilization, in particular to a composite bacterium that anaerobically degrades cellulose and produces methane. Background technique [0002] Natural fibrous substances are the most abundant biomass on the earth, except for a small part that is utilized, most of them exist in the natural environment in the form of waste. In order to fully develop and utilize such abundant renewable resources, some scholars began to study the biodegradation of cellulose as early as 1883 and 1886. The issue of new energy has attracted widespread attention from scholars at home and abroad. [0003] In the natural state, the complete degradation of cellulose is the result of long-term interaction of various microorganisms in the microbial system, and this process cannot be achieved by only one microorganism. Because the pure culture has the following disadvantages: the single enzyme producti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P5/02C12R1/01C12R1/02C12R1/63C12R1/64C12R1/145C12R1/13
CPCY02E50/343Y02E50/30
Inventor 邓宇马诗淳尹小波罗辉刘来雁张辉黎霞刘翔波代丽蓉李强
Owner BIOGAS SCI RES INST MIN OF AGRI
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