Facultative anaerobic denitrifying bacterium with self-flocculation capability and purpose thereof

A denitrifying bacteria and facultative anaerobic technology, applied in the field of facultative anaerobic denitrifying bacteria, can solve the problems of restricting the application of AGS and the long time of granular sludge, and achieve wide applicability, stable effect and promotion of production Effect

Active Publication Date: 2012-10-03
SHANDONG UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of flocculation bacteria in the AGS system, it takes a long time to form granular sludge. For example, it takes 1.5 to 3 months to cultivate AGS in the SBAR reactor, which greatly limits the application of AGS.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Facultative anaerobic denitrifying bacterium with self-flocculation capability and purpose thereof
  • Facultative anaerobic denitrifying bacterium with self-flocculation capability and purpose thereof
  • Facultative anaerobic denitrifying bacterium with self-flocculation capability and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The isolation and identification of embodiment 1 bacterial strain of the present invention:

[0041] (1) Medium:

[0042] A. Strain isolation, purification, preservation medium ( / L):

[0043] CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; Agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0~7.2.

[0044] B. Strain screening, denitrifying medium (DM: Denitrifying Medium) ( / L):

[0045] CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0~7.2.

[0046] C. LB medium ( / L):

[0047] Peptone, 10g; yeast extract, 5g; sodium chloride, 10g; pH is controlled at 7.5.

[0048] (2) Isolation and purification of Pseudomonas stutzeri LZ-4 strain:

[0049] Weigh 10g of rhizosphere soil sample of Arundo donax and put it in a 250mL Erlenmeyer flask, add 100mL of sterile water and a few glass beads, and vibrate on a shaker for 15min to disperse the soil s...

Embodiment 2

[0068] The cultivation of embodiment 2 bacterial strains of the present invention

[0069] (1) Medium used

[0070] A. Strain preservation medium ( / L): peptone, 5g; yeast extract, 3g; glucose, 1g; NaCl, 6g; agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0~7.2.

[0071] B. Strain denitrification medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0~7.2.

[0072] The above medium was sterilized at 121°C for 20 minutes before use.

[0073] (2) Culture conditions

[0074] Use an inoculation loop to scrape 1 ring of bacterial lawn from the Pseudomonas stutzeri LZ-4 preserved on the slant of the preservation medium, inoculate it into a 250mL Erlenmeyer flask containing 100mL of sterilized LB medium, and keep the temperature at 30°C Shake culture for 12 hours to obtain seed solution. During the experiment, the seed liquid was inoculate...

Embodiment 3

[0075] The optimum denitrification condition of embodiment 3 bacterial strains of the present invention

[0076] Put 100mL of sterilized denitrification medium into a 250mL Erlenmeyer flask, insert 10% of the inoculum into the seed bacterial solution, and let it stand for cultivation. In the range of 20-30°C, Pseudomonas stutzeri LZ-4 can remove 95% of total nitrogen, and the removal rate of total nitrogen is the highest at 25°C, reaching 97%. The bacterial strain of the invention can adapt to a relatively wide pH value, and can reduce the total nitrogen of about 150 mg / L to below 10 mg / L between pH 6-11 without nitrite accumulation. The optimum C / N (mol / mol) ratio of the strain of the invention is 3, and when C / N≥3, the removal rate of total nitrogen can reach over 90%. The slightly dissolved oxygen environment of static culture is most suitable for the denitrification activity of the strain of the present invention.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a facultative anaerobic denitrifying bacterium with self-flocculation capability. The bacterial strain is named as Pseudomonas stutzeri LZ-4 and belongs to pseudomonas stutzeri strain, and the preservation number is CCTCCM 2011430. The bacterial strain provided by the invention can be applied to the denitriding of waste water, and the optimal denitriding temperature of thebacterial strain is 20-35 DEG C. The bacterial strain adapts to a wide pH value range which is between pH 6 and pH 11. When a C/N (mol/mol) ratio is more than or equal to 3, the denitriding rate of the bacterial strain is up to above 90%. In a slight oxygen solution environmental condition, the denitriding activity of the bacterial strain is highest. Final product gas of denitrification is authenticated as nitrogen, and inorganic nitrogen such as nitrate nitrogen, nitrite nitrogen and the like in a water body can be directly reduced into the harmless nitrogen to be discharged outside the water body. By using the bacterial strain, at a later period when a denitrification role is played, the aggregation and the formation of a flocculating constituent are started, and the flocculating constituent is precipitated at the bottom of the waste water. Through contrasting the performance detection of the bacterial strain, the following effects that a process of treating nitrogen-containing waste water by using the bacterial strain is simple, a treatment effect is efficient and stable, further, greenhouse gases such as NO and N2O cannot be generated, and the air pollution cannot be caused can be realized.

Description

technical field [0001] The invention relates to a facultative anaerobic denitrification bacterium with self-flocculation ability and application thereof, belonging to the field of environmental microorganisms. Background technique [0002] With the development of industrial and agricultural production and the improvement of people's living standards, the discharge of nitrogen-containing pollutants in my country has increased rapidly, large and small water bodies have become "shelters" for these pollutants, and the quality of water bodies has deteriorated sharply. At present, a large number of wastewater biological treatment projects have been established all over the country, effectively curbing the continuous deterioration of water pollution. However, the nitrogen content of the discharged water after the secondary treatment of sewage is still high, which seems to be a serious source of environmental pollution. Ammonia nitrogen and nitrate nitrogen are the main forms of ni...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F1/52C02F3/34C02F3/28C12R1/38C02F101/16
Inventor 裴海燕胡文容纪雁
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap