Bacterium for degrading phoxim pesticide residue and produced bacterium formulation

A technology for degradation of pesticide residues and phoxim, which is applied in the field of biological high, can solve problems such as use restriction, narrow strain degradation spectrum, etc., and achieve the effects of low production and use cost, good removal effect, and ecological environment protection.

Inactive Publication Date: 2006-07-12
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] Examples of the present invention are described below.

[0017] Activate the original seed of the phoxim pesticide residue-degrading bacteria X-12 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prepared to inoculate seed tanks. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, sterilize under high pressure at 121°C, cool to 33°C, inoculate the above cultured shake flask strains into a 500-liter seed tank at an inoculum size of 10%, and cultivate until the logarithmic growth phase, with a stirring speed of 220 rpm / m...

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Abstract

The invention provides a degradation bacillus which can dispel the phoxime pesticide residue and its prodegradant. The used strain is Gram's staining reaction hysteroptosis X-12 which is Ochrobactrum.sp. The main biology characteristic is G-; the thaliana is the rod type whose size is (1.58-2.61)mu m*(0.79-1.23)mu m; it has flagellation around it and facultative anaerobes; the hydrogen peroxidase, oxidative enzyme, oxidation of ethanol and V.P. reaction is positive; the ketole reaction is negative; it can not be hydrolysed starched and oxide glucose to generate the acid so that the coagulate dydimic acid cow's milk; the Genbank landing number of the strain 16S rDNA is DQ093374.

Description

1. Technical field [0001] The invention discloses a phoxim pesticide residue degrading bacterium and a bacterial agent produced therefrom, belonging to the high-tech field of biology, degrading chemical pesticide residues by means of microorganisms, and being suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases and insect pests for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing a role in the modernization of agricultural production. immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause seriou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/00
Inventor 李顺鹏顾立锋何健洪青张瑞福蒋建东
Owner NANJING AGRICULTURAL UNIVERSITY
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