Facultative incision type recombinant alginate lyase rAly-1 as well as coding gene and application thereof

A technology of alginate lyase and coding gene, applied in the direction of lyase, carbon-oxygen lyase, recombinant DNA technology, etc., to achieve broad application prospects, good temperature stability and pH stability
CN106399285AInactive Publication Date: 2017-02-15WUTONG AROMA CHEM CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUTONG AROMA CHEM CO LTD
Publication Date
2017-02-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to facultative incision type recombinant alginate lyase rAly-1 as well as a coding gene and application thereof. An amino acid sequence of the facultative incision type recombinant alginate lyase rAly-1 is as shown by SEQ ID NO. 2; a nucleotide sequence of the coding gene of the facultative incision type recombinant alginate lyase rAly-1 is as shown by SEQ ID NO. 1. According to the facultative incision type recombinant alginate lyase rAly-1 as well as the coding gene and the application thereof, the facultative incision type recombinant alginate lyase rAly-1 is obtained from a genome of flammeovirga yaeyamensis MY04 for the first time; not only can the lyase be used for degrading a guluronic acid segment from alginate, but also can be used for degrading a mannuronic acid segment. The lyase can be used for thoroughly degrading alginate polysaccharide in any M / G ratio, and efficiently preparing alginate oligosaccharide with a lower polymerization degree.
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Description

technical field

[0001] The invention relates to a facultative endo-cutting recombinant alginate lyase rAly-1 and its coding gene and application, belonging to the technical field of genetic engineering. Background technique

[0002] Alginate is a linear acidic polysaccharide, its main chain molecules are linear, composed of mannuronic acid (Mannuronate, M) and its C-5 differential isomer guluronic acid (Guluronate, G), through glycosidic bonds Linked continuously or alternately. There are uniform poly-M segments, poly-G segments, and hybrid MG or GM segments in the alginate molecule. Alginate is mainly produced from kelp, sargassum and other large marine eukaryotic algae that are used for medicine and food, and is the main component of cell wall and intercellular matrix. In addition, the opportunistic pathogen Pseudomonas aeruginosa and some soil microorganisms such as brown nitrogen-fixing bacteria can also secrete alginate, the difference is that the alginate derived fro...

Claims

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