Preparation of composite bacteria

A production method and technology of composite bacteria, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems that the composition of bacteria cannot be grasped, the culture medium is easy to decline, and the use effect is difficult to control. Achieve the effect of good stability and practical value

Inactive Publication Date: 2009-07-15
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the pure culture has the following disadvantages: that is, the enzyme is single, and the decomposition products of cellulose, cellobiose and glucose, can inhibit the activity of cellulase and inhibit the synthesis of cellulase; the accumulation of volatile acids will easily reduce the pH of the culture solution to Too low to return to conditions suitable for culture growth, resulting in low conversion efficiency of fibrous material
Although these two natural composite strains both hav

Method used

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  • Preparation of composite bacteria
  • Preparation of composite bacteria
  • Preparation of composite bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Mix the following 13 strains of bacteria and inoculate them in the cellulose-containing culture solution, seal them and culture them statically. The culture temperature is 40°C and the pH is 6.5. For: Cytophaga fermentans 10%, Clostridium papyrosolvens 25%, Desulfovibrio vulgaris 1%, Butyrivibrio fibrisolvens 5%, Acetobacterium woodii 5%, Cellulomonas flavigena 1%, Clostridium termitidis 1%, Clostridium cellobioparum 2.5%, Synlfei 5 Womonas %, Methanosarcina barkeri 19%, Methanobrevibacterarboriphilicus 15%, Methanosphaera stadtmaniae 8%, Methanococcus vannielii 2.5%. The formula of the above cellulose-containing culture solution is as follows: NaCl 3‰, cellulose (straw) 5‰, urea 1‰, peptone 1‰, yeast powder 0.5‰, CaCO 3 2‰, with water as the solvent, the above content refers to CaCO 3 , NaCl, cellulose, urea, peptone, and yeast powder as a percentage of the total mass of the culture medium.

[0030] See the following table for the gas production and cellulose degrad...

Embodiment 2

[0033] Mix the following 13 strains of bacteria and inoculate them in the cellulose-containing culture solution, seal them and culture them statically. The culture temperature is 40°C and the pH is 6.5. For: Cytophaga fermentans 7.5%, Clostridium papyrosolvens 35%, Desulfovibrio vulgaris 3%, Butyrivibrio fibrisolyens 4%, Acetobacterium woodii 5%, Cellulomonas flavigena 5%, Clostridium termitidis 3%, Clostridium cellobioparum 5%, Syntropheiphomonas %, Methanosarcina barkeri 10%, Methanobrevibacter arboriphilicus 10%, Methanosphaera stadtmaniae 5%, Methanococcus vannielii 2.5%. The formula of the above cellulose-containing culture solution is as follows: NaCl 5‰, cellulose (straw) 8‰, urea 2‰, peptone 2‰, yeast powder 1‰, CaCO 3 2‰, with water as the solvent, the above content refers to CaCO 3 , NaCl, cellulose, urea, peptone, and yeast powder as a percentage of the total mass of the culture medium.

[0034] See the following table for the gas production and cellulose degrada...

Embodiment 3

[0038] Mix the following 13 strains of bacteria and inoculate them in the cellulose-containing culture solution, seal them and culture them statically. The culture temperature is 40°C and the pH is 6.5. For: Cytophaga fermentans 5%, Clostridium papyrosolvens 30%, Desulfovibrio vulgaris 1%, Butyrivibrio fibrisolvens 5%, Acetobacterium woodii 5%, Cellulomonas flavigena 5%, Clostridium termitidis 2%, Clostridium cellobioparum 3%, Syntrophomonas Wolfei6 %, Methanosarcina barkeri 20%, Methanobrevibacter arboriphilicus 10%, Methanosphaera stadtmaniae 5%, Methanococcus vannielii 3%. The formula of the above cellulose-containing culture solution is as follows: NaCl 7‰, cellulose (straw) 10‰, urea 4‰, peptone 3‰, yeast powder 1.5‰, CaCO 3 2‰, with water as the solvent, the above content refers to CaCO 3 , NaCl, cellulose, urea, peptone, and yeast powder as a percentage of the total mass of the culture medium.

[0039] See the following table for the gas production and cellulose degr...

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Abstract

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

Description

technical field [0001] The invention relates to the technical field of cellulose degradation and energy utilization, in particular to a preparation method of a composite bacterium that degrades cellulose and produces methane. Background technique [0002] Natural fibrous substances are the most abundant biomass on the earth, except for a small part that is utilized, most of them exist in the natural environment in the form of waste. In order to fully develop and utilize such abundant renewable resources, some scholars began to study the biodegradation of cellulose as early as 1883 and 1886. The issue of new energy has attracted widespread attention from scholars at home and abroad. [0003] In the natural state, the complete degradation of cellulose is the result of long-term interaction of multiple microorganisms in the microbial system, and this process cannot be achieved by only one microorganism. Because the pure culture has the following disadvantages: that is, the en...

Claims

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Application Information

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IPC IPC(8): C12N1/22C12R1/01C12R1/02C12R1/63C12R1/145
Inventor 邓宇马诗淳尹小波罗辉刘来雁张辉承磊张云飞代丽蓉李强
Owner BIOGAS SCI RES INST MIN OF AGRI
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