Preparation of composite bacteria

Abstract

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

Description

technical field [0001] The invention relates to the technical field of cellulose degradation and energy utilization, in particular to a preparation method of a composite bacterium that degrades cellulose and produces methane. Background technique [0002] Natural fibrous substances are the most abundant biomass on the earth, except for a small part that is utilized, most of them exist in the natural environment in the form of waste. In order to fully develop and utilize such abundant renewable resources, some scholars began to study the biodegradation of cellulose as early as 1883 and 1886. The issue of new energy has attracted widespread attention from scholars at home and abroad. [0003] In the natural state, the complete degradation of cellulose is the result of long-term interaction of multiple microorganisms in the microbial system, and this process cannot be achieved by only one microorganism. Because the pure culture has the following disadvantages: that is, the en...

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Problems solved by technology

Because the pure culture has the following disadvantages: that is, the enzyme is single, and the decomposition products of cellulose, cellobiose and glucose, can inhibit the activity of cellulase and inhibit the synthesis of cellulase; the accumulation of volatile acids will easily reduce the pH of the culture solution to Too low to return to conditions suitable for culture growth, resulting in low conversion efficiency of fibrous material

Although these two natural composite strains both have strong cellulose degradation ability and methane production ability, the strains contained in this natural fermented bacterial agent are assembled disorderly during the natural passive fermentation process. It is impossible to grasp the composition of the multi-bacteria combination, and it is difficult to control the use effect; moreover, this natural complex bacteria system passively follows the natural circulation law, its decomposition efficiency is not high, and the effect is not stable

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