Preparation of composite bacteria

Abstract

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

Description

technical field [0001] The invention relates to the technical field of cellulose degradation and energy utilization, in particular to a preparation method of a composite bacterium that degrades cellulose and produces methane. Background technique [0002] Natural fibrous substances are the most abundant biomass on the earth, except for a small part that is utilized, most of them exist in the natural environment in the form of waste. In order to fully develop and utilize such abundant renewable resources, some scholars began to study the biodegradation of cellulose as early as 1883 and 1886. The issue of new energy has attracted widespread attention from scholars at home and abroad. [0003] In the natural state, the complete degradation of cellulose is the result of long-term interaction of multiple microorganisms in the microbial system, and this process cannot be achieved by only one microorganism. Because the pure culture has the following disadvantages: that is, the en...

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