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Method for improving hydrogen production quantity of photosynthetic microalgae through phycomycetes co-culture

A technology of co-cultivation and algal bacteria, which is applied in the field of new energy and biological hydrogen production, can solve the problems of high cultivation cost, multiple equipment, and high equipment cost

Active Publication Date: 2018-02-06
CHINA UNIV OF PETROLEUM (EAST CHINA)
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Problems solved by technology

Therefore, this method can only achieve semi-continuous hydrogen production, and the operation process is cumbersome, and the cost of required equipment is high, which makes it impossible to realize large-scale industrial hydrogen production process
That is, in the two-step green algae hydrogen production process, the green algae culture is divided into two steps: "normal culture" and "sulfur-deficiency hydrogen production culture".

Method used

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  • Method for improving hydrogen production quantity of photosynthetic microalgae through phycomycetes co-culture
  • Method for improving hydrogen production quantity of photosynthetic microalgae through phycomycetes co-culture
  • Method for improving hydrogen production quantity of photosynthetic microalgae through phycomycetes co-culture

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Effect test

Embodiment 1

[0046] 1. Cultivation of Chlamydomonas reinhardtii

[0047] A. Select Chlamydomonas reinhardtii species, from the Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;

[0048] B. Cultivate Chlamydomonas reinhardtii:

[0049] (a) Liquid culture conditions of Chlamydomonas reinhardtii: using Tris-Acetate-Phosphate medium (hereinafter referred to as TAP, the formula is shown in Table 1), initial pH 7.2, light intensity level four, and culture temperature 25±1℃, When in liquid culture, it can be cultured statically or placed on a horizontal shaking shaker at 100-120 rpm, and passaged every 5 days.

[0050] (b) Chlamydomonas reinhardtii solid TAP medium: Pour the TAP medium containing 1.5% to 2% agar powder to the plate, streak the plate with an inoculation needle for cultivation, and subculture once every 2 weeks;

[0051] C. Chlamydomonas reinhardtii hydrogen production medium: Chlamydomonas reinhardtii hydrogen production medium uses TAP-S (TAP sulfur-deficient m...

Embodiment 2

[0115] Method for co-cultivating sulfur oxidizing bacteria and chlorella

[0116] A. Co-cultivation method in TAP medium

[0117] Firstly, the chlorella was cultured in the TAP medium to the mid to late log phase, and then the algae was centrifuged at 5000 rpm for 5 min in a centrifuge, and washed with fresh medium for 3 times. The sulfur oxidizing bacteria that grow to the logarithmic phase are first washed three times with TAP medium, and the original medium 688 is removed, and then resuspended in TAP medium to adjust the OD 600 = 1.0. Chlorella (chlorophyll = 0.5mg / L) and sulfur oxidizing bacteria were transferred to hydrogen-producing culture flasks according to 20:1, 40:1, 60:1, 80:1, and 100:1. Each group was set with 3 controls. In addition, sulfur oxidizing bacteria and chlorella were separately set as controls. Finally, put all hydrogen production bottles at 25±1℃, dark treatment for 24h to deplete the original sulfur element, after dark treatment, set the light incubato...

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Abstract

The invention discloses a method for improving the hydrogen production quantity of photosynthetic microalgae through phycomycetes co-culture. Particularly, green alga and one kind of facultative anaerobic / facultative chemoautotrophic thiomonas intermedia are proportionally mixed for culture; oxygen released by green alga photosynthesis can be consumed by the bacterium respiration effect; the carbon dioxide released through the bacterium respiration effect can be supplied to the green alga for better performing photosynthesis effect, so that the anaerobic characteristics of the whole culture environment can be well maintained. In addition, the balance catalysis capability of the thiomonas intermedia on the sulfur element is utilized; the limited supply of the sulfur element can be realized,so that the normal growth of the green alga can be ensured; the efficient durable hydrogen production can be realized.

Description

Technical field [0001] The invention relates to the technical field of new energy and biological hydrogen production, in particular to a method for co-cultivating algae and bacteria to increase the hydrogen production of photosynthetic microalgae. Background technique [0002] Energy has always been an indispensable part of human life, industrial or agricultural production and other social life. It is worth emphasizing that fossil energy such as coal, oil, natural gas, etc., which have limited reserves and are not renewable, are still used by people at this stage. The main part of the irrational mining and use of fossil energy has led to environmental problems such as the greenhouse effect and acid rain. Hydrogen is a clean, high-combustion value, and various forms of renewable bioenergy. According to current preliminary statistics, 95%-96% of hydrogen energy comes from the secondary conversion of fossil energy, and the remaining 4% ~5% is produced by the cracking of water. The ...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P3/00C12R1/01C12R1/89
CPCC12P3/00C12P39/00
Inventor 葛保胜郗丽君贺嘉怡黄方
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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