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Method for treating glucosamine processing wastewater by utilizing fungus and microalgae symbiotic system

A technology for processing wastewater and algae-bacteria symbiosis, applied in microorganism-based methods, methods using spores, biological water/sewage treatment, etc. The effect of secondary pollution

Active Publication Date: 2022-07-15
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the symbiotic relationship between microalgae and fungi is selective. Only suitable strains can grow together and act on sewage treatment together. Unsuitable fungi have an algalytic effect, and the algae cells will grow with the extension of co-cultivation time. while falling

Method used

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  • Method for treating glucosamine processing wastewater by utilizing fungus and microalgae symbiotic system

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Effect test

Embodiment 1

[0041] A method for utilizing a fungal-microalgae symbiotic system to process wastewater from glucosamine processing, comprising the following steps:

[0042] (1) Microalgae pre-culture: Euglena gracilis var. saccharophila was cultured in a 2000ml shake flask with 200ml of Hunter medium three months before use, so that the total number of Euglena gracilis var. saccharophila cells was 1×10 9 100ml of glucosamine processing wastewater was added to the culture system every two weeks. When the volume of the culture solution reached 600ml, all the culture solution was transferred to 10L In the large-mouth glass jar container, add ammonia sugar processing wastewater to the mark, and culture it for 3 days in an outdoor sealed and ventilated container to obtain algal cells;

[0043] (2) Fungal pre-culture: Aspergillus niger and Mucor hiemalis were inoculated into G-YPD medium three months before use, cultured at room temperature for 8 days, and then transferred to a new G-YPD culture ...

Embodiment 2

[0048] A method for utilizing a fungal-microalgae symbiotic system to process wastewater from glucosamine processing, comprising the following steps:

[0049] (1) microalgae pre-cultivation: with embodiment 1;

[0050] (2) Fungal pre-culture: Aspergillus niger and Mucor hiemalis were inoculated into G-YPD medium three months before use, cultured at room temperature for 9 days, and then transferred to a new G-YPD culture In the base, the nutrient components of the original YPD medium are reduced by 20% for each transfer, until the final plate medium contains only two components of glucosamine processing wastewater and agar, and finally the two fungi are collected using sterile 0.05% Tween solution. spore;

[0051] (3) Construct algae-bacteria symbiosis system: inject the slagging and primary precipitation ammonia sugar processing wastewater into the open reaction tank, put the two kinds of fungal spores obtained in step (2) into the ammonia sugar processing wastewater, and thr...

Embodiment 3

[0055] A method for utilizing a fungal-microalgae symbiotic system to process wastewater from glucosamine processing, comprising the following steps:

[0056] (1) microalgae pre-cultivation: with embodiment 1;

[0057](2) Fungal pre-culture: Aspergillus niger and Mucor hiemalis were inoculated into G-YPD medium three months before use, cultured at room temperature for 7 days, and then transferred to a new G-YPD culture In the base, the nutrient components of the original YPD medium are reduced by 20% for each transfer, until the final plate medium contains only two components of glucosamine processing wastewater and agar, and finally the two fungi are collected by using sterile 0.05% Tween solution. spore;

[0058] (3) Construct an algae-bacteria symbiosis system: inject the slagging and primary precipitation ammonia sugar processing wastewater into the open reaction tank, and put the two kinds of fungal spores obtained in step (2) into the ammonia sugar processing wastewater...

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Abstract

The invention discloses a method for treating glucosamine processing wastewater by using a fungus and microalgae symbiotic system, and belongs to the technical field of biological environment protection. According to the method, a fungus and microalgae symbiotic system is constructed, euglena gracilis, black mould and mucor are domesticated to form phycomycete microspheres, so that the sewage treatment effect is guaranteed, phycomycetes can be naturally settled, the treatment capacity of centrifugal recovery is reduced, and meanwhile, the problem of pipeline blockage caused by suspension of the phycomycetes is avoided. The phycomycete symbiotic system is used for directly treating the glucosamine processing wastewater, the existing process of firstly carrying out chemical treatment and then carrying out biological treatment is replaced, and the problem of secondary pollution caused in the use process of chemical reagents is avoided. The glucosamine processing wastewater treated by the method disclosed by the invention meets the water pollutant discharge standard of the extraction type pharmaceutical industry, and has important promotion significance for promoting glucosamine processing production.

Description

technical field [0001] The invention relates to a method for using a fungal-microalgae symbiotic system to process waste water from glucosamine processing, and belongs to the technical field of biological environment protection. Background technique [0002] Glucosamine (GlcN), full name glucosamine, molecular formula C 6 H 13 NO 5 , which is formed by the substitution of a hydroxyl group of glucose by an amino group, and usually exists in the form of polysaccharide or conjugated polysaccharide in shrimp and crab shells and fungal cell walls in nature. This organic substance is necessary for the synthesis of proteoglycan in human articular cartilage matrix, and its chondroitin agent is widely used in the prevention and treatment of bone and joint diseases. [0003] However, the development of GlcN industry faces enormous environmental pressure. The raw material library for GlcN preparation is mainly shrimp shells. The main preparation process includes: decalcification, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32C02F9/14C12N1/10C12N1/14C12N3/00C02F103/36C12R1/685C12R1/785
CPCC02F3/325C02F9/00C12N1/10C12N1/14C12N3/00C02F2103/36C02F1/001C02F2001/007C02F1/66C02F7/00
Inventor 韩志萍彭元怀葛歆王胜杨娟
Owner LINGNAN NORMAL UNIV
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