Novel constructed high-yield fumaric acid gene engineering bacterium and method for producing fumaric acid thereby

Abstract

The invention discloses a method for constructing genetic engineering bacterial strain Escherichia coli JM125 producing boletic acid and method for producing acid. The constructing process comprises inactivation or out-knocking enzymes in facultative microbiology which has abilities of reduction boletic acid and degrading acetonic acid, and exceeding expressing NAD regeneration. The following steps of two-stage fermentation experiment using constructed Escherichia coli are (1) activating bacillus coli is transferred to enrichment medium and cultured to improve biomass. (2) The treated bacterium liquid membrane obtained by aerobic cultivation is transferred into LB culture medium which contains 20 to 100g / L amylaceum and certain concentration carbonates to produce fumaric acid by anaerobic fermentation.

Description

Technical field

[0001] The invention belongs to the technical field of bioengineering, relates to the construction of a fumaric acid-producing recombinant strain, and also relates to a method for fermentative production of fumaric acid by using the strain. Background technique

[0002] Fumaric acid (also known as fumaric acid), as an important four-carbon platform compound, has a wide range of uses in the pharmaceutical, food, and surfactant industries. It can be used to produce L-day through enzyme-catalyzed conversion, esterification, and hydrogenation. Four-carbon compounds such as aspartic acid, malic acid, succinic acid, maleic acid, 1,4-butanediol, γ-butyrolactone and tetrahydrofuran. At present, fumaric acid is mainly produced through the isomerization of maleic anhydride and furfural oxidation. Due to the high cost and environmental pollution of chemical synthesis of fumaric acid, the wide application of fumaric acid as a basic chemical raw material is restricted. [0003...

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