Method for synthesizing 1,3-propanediol by biological method

A technology of propylene glycol and biological method, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low conversion rate, high cost, complicated process, etc., and achieve short fermentation cycle, easy operation, and high production intensity high effect

Inactive Publication Date: 2009-12-16
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for biologically synthesizing 1,3-propa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Aseptically inoculate the facultative anaerobic Klebsiella pneumoniae preserved in glycerol tubes onto LB (Luria-Bertani) solid medium, and activate and cultivate at 30°C for 8 hours; wherein each liter of LB medium contains Yeast extract powder 5g, peptone 10g, NaCl 5g, agar 15g, adjust medium pH to 7.0 with sodium carbonate;

[0021] 2) Pick a single colony on the surface of the solid medium obtained after the previous step of cultivation, insert it into 150mL seed medium, the culture container is a 500mL shaker flask, cultivate it on a shaker at 30°C for 8 hours, and the shaker speed is 180rpm to obtain a first-grade Seed liquid; the first-class seeds obtained are inserted into the seed medium under aseptic conditions, and the inoculum amount of the seed liquid is 5-10% of the medium, and cultivated under the same conditions to obtain the second-level seed liquid; wherein the seed medium is Glycerin 20g, yeast powder 1g, (NH 4 ) 2 SO 4 6g, K 2 HPO 4 ·3H 2 O ...

Embodiment 2

[0029] 1) with the first step of embodiment 1

[0030] 2) with the second step of embodiment 1

[0031] 3) Same as the third step of Example 1, but during the fermentation process, the pH value of the fermentation broth was adjusted by 4M sodium hydroxide, glycerol was added at a constant rate of 2.2 g / L·h, and the fermentation was carried out for 36 hours.

[0032] Fermentation result:

[0033] During the fermentation process, the consumption rate of glycerol concentration was relatively stable in the first 20 hours, and slowing down the accumulation of glycerol in the later period affected the synthesis of 1,3-propanediol. After 36 hours of fermentation, the final concentration of 1,3-propanediol was 36.02g / L, and the molar conversion rate 0.62, the production intensity is 1.0g / L·h. Compared with the fermentation without feeding, although the concentration of the product was increased, the method of feeding glycerol at a constant rate made the synthesis of 1,3-propanediol ...

Embodiment 3

[0035] 1) Same as the first step in Example 1

[0036] 2) Same as the second step of Example 1

[0037] 3) The same as the third step of Example 1, the feeding solution is a mixed solution of glycerin and alkali (potassium hydroxide or sodium hydroxide) with a molar ratio of 2.5:1. No ventilation during fermentation. Ferment for 36 hours.

[0038] Fermentation result:

[0039] The real-time feed-fed fermentation of glycerol with glycerol pH regulation was carried out under the condition of no ventilation, and the final concentration of 1,3-propanediol was increased to 55.76g / L after 36 hours of fermentation, and the average production intensity was 1.55g / l·h, but glycerol had no effect on 1,3-propanediol. The molar conversion of propylene glycol decreased to 0.62. Although the airless method can reduce a large amount of power consumption, metabolic waste such as carbon dioxide produced by microbial metabolism cannot be discharged during the fermentation process, which affe...

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PUM

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Abstract

The invention provides a production process for synthesizing 1,3-propanediol by a biological method, and belongs to the technical field of biochemical engineering. The process adopts microbe aerobic fermentation, uses amphimicrobian Klebsiella pneumoniae as a starting strain, and comprises the following steps in turn: activating seeds of the Klebsiella pneumoniae; culturing the seeds under an aerobic condition; under a microaerobic condition, using thallus of the Klebsiella pneumoniae as a biocatalyst, and fermenting a glycerinculture-medium assisted by glucose to produce the 1,3-propanediol under conditions that the fermenting temperature is between 30 and 50 DEG C and pH is between 7 and 9; and finishing fermentation after fermenting for 18 to 36 hours. The method for synthesizing the 1,3-propanediol has the characteristics of short production period, high production strength, simple fermenting control, convenient operation and low cost.

Description

technical field [0001] The invention relates to a method for biologically synthesizing 1,3-propanediol, which belongs to the technical field of biochemical industry. Background technique [0002] 1,3-propanediol (1,3-propanediol) is an important chemical raw material, which can be used as an important monomer raw material for polyester, polyether and polyurethane, and is one of the important products studied in the field of industrial biotechnology. Polymers synthesized from 1,3-propanediol have excellent characteristics such as biodegradability, safety and non-toxicity, which are not possessed by many other glycol synthetic plastics. They are not only used in the field of clothing and engineering plastics, but also used as Cosmetic packaging materials began to be applied. Propylene glycol ester, a food additive synthesized from 1,3-propanediol, is one of the six major food emulsifiers in the world. It has also been approved for use by the United States, Japan, China and ot...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/22
Inventor 李春徐小琳张根林马彬彬吕波
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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