Method for detecting melamine and specific enzyme-linked immunologic reagent kit

An enzyme-linked immunosorbent reagent and melamine technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unsuitable for on-site monitoring sample screening, high skill requirements for inspectors, and complicated equipment and equipment, so as to shorten the detection time Time-saving, easy-to-use, high-sensitivity effects

Inactive Publication Date: 2009-04-22
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the conventional detection methods of melamine residue mainly include liquid chromatography / mass spectrometry (LC / MS), high performance liquid chromatography (HPLC), liquid chromatography tandem mass spectrometry (LC / MSMS), etc. It is not suitable for on-site monitoring and screening of a large number of samples due to the shortcomings of complexity, cumbersome testing process and high skill requirements for inspectors

Method used

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  • Method for detecting melamine and specific enzyme-linked immunologic reagent kit
  • Method for detecting melamine and specific enzyme-linked immunologic reagent kit
  • Method for detecting melamine and specific enzyme-linked immunologic reagent kit

Examples

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Effect test

Embodiment 1

[0037] Example 1. Preparation and use of a kit using a hapten-carrier protein conjugate as a coating source and an enzyme-labeled secondary antibody as an enzyme marker

[0038] 1. The detection principle of the kit with the melamine hapten and carrier protein conjugate as the coating source and the enzyme-labeled secondary antibody as the enzyme marker is as follows:

[0039] When the coating on the microwell strip of the microtiter plate was originally a conjugated melamine hapten and carrier protein, after adding the standard solution or sample solution to the microwell of the microtiter plate, the melamine-specific antibody was added, and the residue in the sample The melamine on the ELISA plate competes with the melamine-coupled antigen for the melamine-specific antibody, and then the enzyme-labeled anti-antibody is added for amplification, and the color is developed with a chromogenic solution. The absorbance value of the sample is negatively correlated with the content o...

Embodiment 2

[0141] Embodiment 2, the kit that is used to detect melamine can also have following several kinds:

[0142] 1. The original coating is a specific antibody, and the enzyme marker is an enzyme-labeled hapten kit

[0143] (1) The working principle of this kit is:

[0144]When the melamine-specific antibody described in Example 1 is pre-coated on the microwell strip, after adding the sample solution or standard solution, add the enzyme-labeled hapten solution. The melamine or melamine standard in the sample competes with the enzyme-labeled antigen for the melamine-specific antibody coated on the microtiter plate, and the color is developed with a chromogenic solution. The absorbance value of the sample is negatively correlated with the content of melamine in the sample, and compared with the standard curve The content of melamine in the sample can be obtained. At the same time, the concentration range of melamine in the sample can be roughly judged by comparing the color of the...

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Abstract

The invention discloses a method for detecting melamine and a special enzyme-linked immunosorbent assay kit thereof. The enzyme-linked immunosorbent assay kit for detecting the melamine comprises a hapten and a specific antibody of the melamine; and the specific antibody is the polyclonal antibody or the monoclonal antibody of the melamine. The kit adopts the high specific monoclonal antibody of the melamine, thereby ensuring the reliability of test results; the test results show that the kit is characterized by high specificity, high sensitivity, high precision and high accuracy; main reagents of the kit all adopt the form of working liquid, thereby having convenient use and low cost. The method for detecting the melamine by using the kit has simple operation, low requirements on the pre-treatment of samples and can rapidly detect large quantities of samples. Therefore, the use of the method for detecting by using the enzyme-linked immunosorbent assay kit can monitor on-site and be applicable to qualitative and quantitative screening of a large number of samples.

Description

technical field [0001] The invention relates to a method for detecting melamine and a special ELISA kit. Background technique [0002] Melamine is a triazine nitrogen-containing heterocyclic organic compound, its structural formula is as follows figure 1 shown. Its nitrogen content is as high as 66%, and it is white single crystal oblique crystal, odorless. Because of its easy purchase and production and low cost, inherently unscrupulous manufacturers aim at the shortcomings of the Kjeldahl nitrogen method and add it to food and feed to increase protein content and gain benefits. Although melamine is a low-toxic compound, it will be metabolized into cyanuric acid in animals, and melamine and cyanuric acid will react and accumulate, which can cause bladder stones and urinary tract lesions. Long-term stimulation of bladder stones can lead to bladder tumors. Therefore, it is necessary to establish a method for detecting melamine with high precision and high sensitivity in p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/577C12N5/12
Inventor 何方洋万宇平冯才伟赵正苗冯才茂汪善良
Owner 贵州勤邦食品安全科学技术有限公司
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