37 results about "Gene switch" patented technology

The present invention relates to methods and compositions for treating, ameliorating or preventing a disease or disorder in a subject by introducing into cells of the subject a therapeutic gene switch construct that controls expression of one or more therapeutic products.

The invention relates to an insect steroid receptor protein which is capable of acting as a gene switch which is responsive to a chemical inducer enabling external control of the gene.

The invention relates to a new “gene-switch” (cumate-inducible switch) for mammalian cells. This switch is as useful in the development of expression systems and cell-based assays for functional genomics as in the generation of viral vectors for gene therapy.

This invention discloses one EPSPS gene enzyme immune agent case and its use method in the anti-herbicide bean, wherein, the case comprises anti-EPSPS multi-clone antigen, enzyme board with the antigen, enzyme antigens for two, gene switch bean standard product, non-transfer gene bean standard, intense wash liquid, develop liquid and terminal liquid. This invention method comprises the following steps: cloning CP4-EPSPS gene from the gene switch bean expressed in the bacillus coli; then through protein purification complex property to regroup EPSPS protein antibody immune animal to get special single clone antigen and multi-clone antigen; establishing double antigen clamp enzyme immune system test sample to determine its EPSPS protein content.

Provided is an improved method for controlling gene expression in vivo through the use of a gene switch comprising one or more aptamer sequences operably linked to or incorporated into the untranslated regions (UTRs) of a transgene or nucleotide sequence of interest. Also provided are expression vectors having aptamer sequences located within the 3′ UTR, the 5′ UTR and between the genes of a multicistronic mRNA, as well as methods of using the expression vectors for controlling or regulating gene expression.

The present invention relates to conditionally replicating viruses or pairs of viruses containing a gene switch that is activatable by transient heat or other proteotoxic stress in the presence or absence of a small molecule regulator. The gene switch controls the expression of a gene for a protein required for efficient viral replication and may also control the activity of a passenger gene.

The present invention relates to steroidal ligands for use in nuclear receptor-based inducible gene expression systems. The invention further relates to methods of modulating the expression of genes of interest with a system containing one or more nuclear receptor complexes and one or more steroidal ligands. Further aspects include ligand compositions including therapeutic compositions.

Single chain, monomeric polypeptide gene switches are provided. The gene switches include ligand binding domains and at least one functional domain. Preferred functional domains are DNA binding domains and transcriptional regulating domains. Methods of regulating gene function using the switches are also provided.

The present invention relates to an orthogonal gene switch for regulating the expression of a desired gene. The gene switch comprises a chimeric transcription factor that does not respond to endogenous ligands, and a ligand that is capable of activating the chimeric transcription factor but not endogenous transcription factors. The present invention also relates to the method of constructing the orthogonal gene switch.

Disclosed is a novel inducible expression system characterized by undetectable biological effect in the absence of an inducer, but which exhibits efficient inducibility in the presence of an inducer.

The present invention relates to conditionally replicating viruses or pairs of viruses containing a gene switch that is activatable by transient heat or other proteotoxic stress in the presence or absence of a small molecule regulator. The gene switch controls the expression of a gene for a protein required for efficient viral replication and may also control the activity of a passenger gene.

Provided is a seed sorting method for fixing plant heterosis. The invention provides a method for controlling and screening marker genes by a pollen specific genetic switching system and then sortingand cloning seeds so as to realizing the application of a hybrid seed non-fusion system. Three closely linked gene expression cassettes and a MiMe knockout vector are simultaneously transferred into ahybrid plant. The three gene expression cassettes include: 1) an embryonic autonomous gene expression cassette E1 driven by an oocyte specific expression promoter; 2) a screening marker gene expression cassette E2 regulated by E3; and 3) an expression cassette E3 for pollen-specific control of E2. In selfing fruiting seeds of hybrid transgenic plants, besides asexual embryo seeds which are fixedwith heterosis and are generated through parthenogenesis, zygotic embryo seeds formed by pollination and fertilization are also included; the two kinds of seeds are sorted by screening markers controlled by a pollen-specific gene switch, cloned seeds retain heterosis and can be used for production, and the remaining seeds can be used for commercial purposes.

The invention discloses an enterococcus faecalis quorum sensing gene switch system constructed in escherichia coli as well as expression vector, engineering bacteria and application thereof. The enterococcus faecalis quorum sensing gene switch system comprises a heptapeptide pheromone cCF10 gene ccfa of enterococcus faecalis, a negative regulation pheromone peptide gene prgx and a promoter sequence Pprgq. The gene switch system provided by the invention is a gene switch system which does not depend on an inducer and can spontaneously and dynamically adjust target gene expression, fermentationengineering bacteria capable of starting target gene expression when OD600 reaches 1 are constructed by using the system. According to the inventioin, the starting strength is 10 times higher than that of the current commonly used vibrio fischer quorum sensing system. According to the invention, gene elements are respectively constructed on different expression vectors, and a gene switch system isconstructed in engineering bacteria, so that the engineering bacteria have the functions of spontaneously and dynamically regulating metabolic flow of fermentation strains and controlling gene expression, so that addition of an inducer can be avoided, the fermentation cost is saved and the fermentation process is simplified.

Disclosed herein are methods and compositions relating to gene switches that use molecule capable of binding DNA sequences.

Disclosed herein are polynucleotides encoding ligand-inducible gene switch polypeptides, and systems comprising gene switch polypeptides for modulating the expression of a heterologous gene and an interleukin in a host cell. The compositions, methods and systems described herein facilitate ligand dependent expression of polypeptides including but not limited to cytokines and antigen binding polypeptides.

Mutants of steroid receptor ligand binding domains and synthetic ligands which have specific binding affinities for these receptors are provided. The use of these LBD-ligand combinations for construction of selective “molecular gene switches” is disclosed. Methods of regulating gene function using these switches are provided.

The present invention relates to steroidal ligands for use in nuclear receptor-based inducible gene expression systems. The invention further relates to methods of modulating the expression of genes of interest with a system containing one or more nuclear receptor complexes and one or more steroidal ligands. Further aspects include ligand compositions including therapeutic compositions.

The present invention relates to methods and compositions for treating, ameliorating or preventing a disease or disorder in a subject by introducing into cells of the subject a therapeutic gene switch construct that controls expression of one or more therapeutic products.

The invention discloses a gene switch system for directional expression of insect-resistant and / or herbicide-resistant genes of corn as well as a construction method and application of the gene switch system. The gene switch system comprises a locking element containing the insect-resistant and / or herbicide-resistant genes, and a nucleotide sequence which opens the locking element as shown in SEQ ID NO.1 and enables the locking element to be expressed in specific tissue, wherein the nucleotide sequence shown as SEQ ID NO.1 is a site-specific recombinase gene Cre. An endogenous green tissue specific promoter of the corn is utilized, a site specific recombination system and related regulation elements are combined, a corn gene directional expression 'gene switch ' technical platform is constructed, precise expression of exogenous genes is achieved, and powerful technical support is provided for cultivation of high-quality, safe and efficient new transgenic crop strains.

The invention provides methods for detecting and analyzing protein-protein interactions and agonists and antagonists thereof, detecting and analyzing protein sequences, and regulatable gene expression in multicellular organisms or cells therefrom.

The invention relates to a new “gene-switch” (cumate-inducible switch) for mammalian cells. This switch is as useful in the development of expression systems and cell-based assays for functional genomics as in the generation of viral vectors for gene therapy.

Provided are: a selection method for a gene switch and a gene circuit, including using, as a selector, an expression vector containing at least a gene sequence whose expression is controlled by a transcription regulatory factor to be expressed when a genetic switch and a genetic circuit including the genetic switch operate, and a promoter sequence operably linked to the gene sequence upstream thereof; and an expression vector to be used in the selection method. This enables an effective selection method for a genetic switch and a genetic circuit, the selection method being able to be conducted within a short time period and with high selection efficiency and less leakiness.

Disclosed herein are polynucleotides encoding ligand-inducible gene switch polypeptides, and systems comprising gene switch polypeptides for modulating the expression of a heterologous gene and an interleukin in a host cell. The compositions, methods and systems described herein facilitate ligand dependent expression of polypeptides including but not limited to cytokines and antigen binding polypeptides.

The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.

Compositions and methods are provided which employ a chemical-gene switch. The chemical-gene switch disclosed herein comprises at least three components. The first component comprises a polynucleotide encoding a chemically-regulated transcriptional repressor; the second component comprises a repressible promoter operably linked to a polynucleotide of interest, and the third component comprises a gene silencing construct operably linked to a second repressible promoter, wherein the gene silencing construct encodes a silencing element that decreases the level of mRNA encoding the chemically -regulated transcriptional repressor. Expression of the polynucleotide of interest and the silencing construct is controlled by providing the appropriate chemical ligand. Transient induction from the chemical ligand leads to the production of the silencing element, and the destruction of the mRNA encoding the chemically-regulated transcriptional repressor. The presence of the silencing element maintains a state of de-repression. In specific embodiments, the silencing elements are cell non-autonomous, the state of de-repression becomes more distributed throughout the plant beyond where the chemical ligand reaches.

The present invention relates to conditionally replicating viruses or pairs of viruses containing a gene switch that is activatable by transient heat or other proteotoxic stress in the presence or absence of a small molecule regulator. The gene switch controls the expression of a gene for a protein required for efficient viral replication and may also control the activity of a passenger gene.

The invention provides methods for detecting and analyzing protein-protein interactions and agonists and antagonists thereof, detecting and analyzing protein sequences, and regulatable gene expression in multicellular organisms or cells therefrom.