18 results about "Signalling cascade" patented technology

The present invention establishes a method for screening for, on the basis of a melanophagy-regulated signaling cascade involving PTK2, ITCH, MLANA and OPTN, a substance exhibiting melanophagy inhibitory activity, and provides a composition for preventing, mitigating, alleviating or treating pigmentation-related diseases, containing an active substance discovered using the developed screening method. The screening method of the present invention can effectively identify, on the basis of a novel melanophagy-regulated signaling cascade, the usefulness and potential utility of a substance exhibiting melanophagy inhibitory activity, and thus has a high utility value as a platform technology for melanophagy regulation. The composition according to the present invention has an excellent melanophagy inhibitory effect, and thus can be used in various fields such as pharmaceuticals, cosmetics and health functional foods.

The invention belongs to the technical field of nucleic acid molecule detection, and particularly relates to an enzyme-free isothermal strand displacement cascade amplification reagent for acute respiratory pathogen detection and application of the enzyme-free isothermal strand displacement cascade amplification reagent. The detection method based on the amplification reagent is based on a nucleic acid reaction system composed of an initiation double strand and two molecular beacons, cyclic amplification is performed through a toehold-mediated strand displacement reaction trigger signal under a constant temperature condition, and end point interpretation is performed through a fluorescence signal. The method does not need enzyme and thermal cycle, is short in detection time, adapts to high-throughput operation of a porous plate, and realizes specific recognition and signal cascade amplification of target nucleic acid under a constant temperature condition, so that rapid, accurate and high-throughput detection of acute respiratory pathogens is realized on the premise of not depending on enzymatic reaction and thermal cycle equipment. In addition, the kit has the advantages of low cost, good stability and wide application range, and is suitable for rapid detection of various acute respiratory pathogens.

The invention discloses a glyphosate fluorescence detection method based on RNase H cycle amplified magnetic bead-aptamer-Cas9 / deoxyribozyme, belongs to the technical field of environmental monitoring and biological detection, and can realize high-sensitivity and rapid quantitative detection of glyphosate residues in soil, water bodies and agricultural products. According to the method, the glyphosate is combined with an aptamer to release complementary DNA (cDNA) combined with the aptamer, then signal cascade amplification is realized through RNase H-mediated cDNA cyclic utilization, the anti-interference advantage brought by magnetic bead separation is combined, and the known characteristics of Cas9 endonuclease and DNAzyme in the aspects of nucleic acid recognition and cutting are used as signal amplification and transduction means, so that the detection sensitivity of the glyphosate is improved, and the detection sensitivity of the glyphosate is improved. Therefore, trace detection of glyphosate is realized. According to the invention, sensitive detection of other targets can be realized by replacing nucleic acid aptamers capable of specifically recognizing other targets and correspondingly adjusting the sequence of cDNA to maintain base pairing.

The invention discloses a nutrition regulation and control method for disease resistance of kiwi fruits, a germination fertilizer and a preparation method, and the preparation method comprises the following steps: constructing a ternary interaction system consisting of a sulfur source, succinic acid and Pseudomonas fluorescens, and applying the ternary interaction system to rhizosphere nutrition management of the kiwi fruits in early spring. And three-dimensional coupling of nutrition supply, signal activation and microbial regulation and control is synchronously realized in the rhizosphere microdomain. Meanwhile, lignin monomer synthesis is continuously activated and promoted through a salicylic acid signal channel, high-activity laccase serves as a key enzyme for lignin monomer polymerization, lignin monomer polymerization reaction is accelerated, synthesis and deposition of lignin in plant cell walls are promoted, plant endogenous disease-resistant signal cascade is continuously amplified, and plant disease resistance is improved. The kiwi fruits are induced to generate stable systematic disease resistance. The composition can prevent and control diseases for a long time and cooperatively promote plant growth.

The present application relates to the technical field of nanobiomaterials, and particularly relates to a nano-polypeptide material for double inhibition of a tumor Wnt signal pathway, a preparation method and application thereof, the nano-polypeptide material for double inhibition of the tumor Wnt signal pathway comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO:1, and a hydrophobic fluorescent group connected to a terminal lysine of the polypeptide through an amide bond. After the nano-polypeptide material targets FZD7 proteins highly expressed on a tumor cell membrane surface, the nano-polypeptide material is deformed into a nano-fiber network structure in situ, long-term retention and direct blocking of a binding site of a Wnt ligand and the FZD7 protein are achieved, and signal activation is inhibited from an upstream of the pathway. Meanwhile, the dense fiber network indirectly limits a self-secretion and paracrine process of mature modified Wnt proteins mediated by vesicles or exosomes, signal cascade amplification conduction between adjacent cells is inhibited, key protein expression and downstream target protein transcription levels in the Wnt signal pathway are double down-regulated in the inside and outside, and finally the treatment effect of the tumor is enhanced.

This disclosure provides a method for alleviating aluminum toxicity in hydroponic peas based on menadione-mediated reactive oxygen species (ROS) metabolism. The core components include: regulating the concentration and duration of menadione treatment to optimize the spatiotemporal accumulation pattern of ROS (superoxide anion, hydrogen peroxide) in the roots of hydroponic peas; adjusting the dynamic response of endogenous antioxidant enzyme systems (SOD, POD, APX) to enhance menadione-mediated ROS scavenging capacity; controlling the initial ROS burst based on changes in plasma membrane NADPH oxidase (Rboh) gene expression and enzyme activity to inhibit the aluminum stress oxidative signaling cascade; and adjusting the timing and frequency of menadione administration based on subcellular-scale mitochondrial and chloroplast ROS leakage rates to alleviate organ-specific oxidative damage. This approach addresses five key issues: aluminum-induced oxidative damage, cellular redox imbalance, oxidative signaling cascade, organ-specific oxidative damage, and persistent aluminum toxicity metabolic disorders.

The application discloses a D-p driving probe and a Cas12a cascade amplification method based on the probe, and belongs to the technical field of molecular diagnosis. The basic structure of the D-p driving probe is a hairpin type DNA with a breathing site, which is composed of a double-stranded stem and a single-stranded loop. The single-stranded loop is a trans-cleavage region. The double-stranded stem includes an activator, a foothold, a blocking region and a breathing site. In the above manner, the D-p driving probe has the dual functions of Cas12a trans-cleavage substrate and target activator, can connect the target recognition and trans-cleavage activity of Cas12a in series, realizes efficient signal cascade amplification, and simultaneously introduces the breathing site in the D-p driving probe, so that only the activator foothold is exposed after cutting, without affecting the key functional region, thereby avoiding false cutting and guaranteeing effective signal transmission. In addition, the ortho-allosteric activation mechanism based on the D-p driving probe can significantly inhibit the off-target effect of Cas12a and effectively improve the recognition specificity.

The invention relates to the technical field of nano biological materials, in particular to a nano polypeptide material for dual inhibition of tumor Wnt signal pathways as well as a preparation method and application of the nano polypeptide material for dual inhibition of tumor Wnt signal pathways, and the nano polypeptide material for dual inhibition of tumor Wnt signal pathways comprises polypeptide with an amino acid sequence as shown in SEQ ID NO: 1, the hydrophobic fluorophore is connected with the last lysine of the polypeptide through an amido bond. The nano polypeptide material deforms into a nano fiber network structure in situ after targeting FZD7 protein highly expressed on the surface of a tumor cell membrane, is retained for a long time and directly blocks a binding site of a Wnt ligand and the FZD7 protein, and inhibits signal activation from the upstream of a pathway. Meanwhile, the dense fiber network indirectly limits autosecretion and paracrine processes mediated by vesicles or exosomes of mature modified Wnt protein and inhibits signal cascade amplification conduction between adjacent cells, so that key protein expression and downstream target protein transcription level in a Wnt signal channel are doubly down-regulated in internal and external aspects, and finally, the tumor treatment effect is enhanced.

The present invention is directed to novel modulators of the Ca2+-signaling cascade, acting as potentiators of the P2RY2 purinergic receptor and of the inositol triphosphate receptors, ITPRs. The compounds are useful in the treatment of diseases and conditions in which modulation of Ca2+-signaling plays a role, such as in some forms of spinocerebellar ataxia, and in diseases and conditions benefiting from mucosal hydration, as for example respiratory diseases and conditions, dry eye, xerostomia.

The invention provides a method for detecting a cancer marker miR-155 by using a biosensor for dynamically regulating and controlling Cas12a trans-cleavage activity by utilizing catalytic hairpin assembly mediated transcription pre-crRNA. A transcription template containing a T7 promoter is formed by catalyzing a hairpin assembly (CHA) reaction, so that self-supply of pre-crRNA is realized. Specifically, pre-crRNA and trigger are hybridized to form a compound, after the compound is combined with Cas12a protein, the self-processing function of the pre-crRNA is triggered, and then the cleavage activity of Cas12a is reassembled and activated, so that regulation and control of the Cas12a are realized. A detection signal is amplified in a cascade manner by combining a catalytic hairpin assembly reaction with a T7RNA polymerase mediated signal amplification module, so that high-sensitivity and high-specificity detection of miR-155 is realized, and the detection limit reaches 86 aM. The sensor shows good reliability, low background interference, operability and programmability in a complex sample, and can be applied to detection of miR-155 in tumor cells.

The invention discloses a D-p driving probe and a Cas12a cascade amplification method based on the D-p driving probe, and belongs to the technical field of molecular diagnosis, the basic structure of the D-p driving probe is hairpin type DNA with respiration sites, the D-p driving probe is composed of a double-stranded stem part and a single-stranded loop part, and the single-stranded loop part is a trans-shear region; the double-stranded stem includes an activator, a foothold, a closure region, and a breathing site. Through the mode, the D-p driving probe has dual functions of a Cas12a trans-cleavage substrate and a target activator, and target recognition and trans-cleavage activity of Cas12a can be connected in series, so that efficient signal cascade amplification is realized; meanwhile, a breathing site introduced into the D-p driving probe enables only an activator footing point to be exposed after cutting, and a key functional area is not affected, so that mistaken cutting is avoided, and effective transmission of signals is guaranteed; in addition, an ortho-position allosteric activation mechanism based on the D-p driving probe can significantly inhibit the off-target effect of Cas12a, and the recognition specificity is effectively improved.

This invention relates to a double-antibody sandwich ELISA kit for detecting serum PTGS1 protein, comprising: a PTGS1 capture antibody, an unlabeled PTGS1 detection antibody, an enzyme-labeled IgG secondary antibody, a solid-phase carrier, an enzyme substrate, a blocking buffer, a washing buffer, and a stop solution; the IgG secondary antibody specifically binds to the PTGS1 detection antibody; the PTGS1 capture antibody is a rabbit monoclonal antibody that specifically binds to the linear epitope A of human PTGS1 protein, the amino acid sequence of which is SEQ ID NO:1, corresponding to amino acids 271-282 of human PTGS1 protein; the PTGS1 detection antibody is a mouse monoclonal antibody that specifically binds to the B epitope of human PTGS1 protein; the amino acid sequences of the B epitope at the A epitope do not overlap and do not constitute steric hindrance. This kit avoids the conserved homologous region between PTGS1 and its homologous protein PTGS2, ensuring the accuracy of the capture antibody recognition, and uses an indirect detection mode with enzyme-labeled goat anti-mouse IgG secondary antibody to achieve signal cascade amplification, significantly improving detection sensitivity.

The present application relates to the field of nucleic acid detection, and particularly relates to a novel nucleic acid detection method based on CRISPR-Cas12. By introducing chemical modification (such as locked nucleic acid modification), the present application constructs a method for site-specific cleavage of specific phosphodiester bonds in single-stranded nucleic acid by Cas protein containing RuvC domain and a method for detecting target DNA in a sample to be tested, so as to realize site-specific cleavage and trigger signal cascade amplification, and then realize rapid and high-sensitivity detection of nucleic acid.

The invention discloses a self-assembly fluorescent biosensing system for detecting DNA of sulfate reducing bacteria, and also provides a construction method and application of the system. According to the sensing system, a three-dimensional DNA nanoflower prepared by a rolling circle amplification technology is used as a self-assembly substrate, and a DNA template CdTe quantum dot synthesized by a one-pot method is used as a signal probe; dNA template quantum dots are self-assembled on the surface of the three-dimensional DNA nanoflower through the complementary pairing effect of bases, and construction of a fluorescent biosensing system is completed. In the presence of the target DNA, the system can realize specific recognition and signal cascade amplification of the target, so that the fluorescence intensity of the system is proportionally enhanced along with the change of the concentration of the target DNA, thereby realizing high-sensitivity and high-specificity qualitative and quantitative detection of the DNA of the sulfate reducing bacteria. The sensing system does not need to carry out complex surface modification treatment on the quantum dots, has good stability and repeated detection performance, and has good application prospects in the fields of rapid diagnosis of pathogenic microorganisms, detection of environmental microorganisms and the like.

The targeted delivery of growth factors, vasoactive peptides and other representative anabolic peptide drugs from different signaling cascades to bone fracture for accelerated healing is disclosed herein.

The application discloses application of ENOPH1 gene and relates to the technical field of biological medicine. In the application, bioinformatics analysis is adopted to identify key metabolic genes closely related to CRC prognosis, and expression of ENOPH1 in KRAS mutant CRC tissues and cell lines is evaluated. Through in vitro experiments such as CCK8, wound healing experiment and subcutaneous xenotransplant tumor model in vivo, the function of ENOPH1 in KRAS G12D / G13D mutant CRC is verified. The results show that ENOPH1 is highly expressed in KRAS mutant CRC and is controlled by MEK / ERK signal cascade. Through shRNA knockdown of ENOPH1, the proliferation, migration and tumorigenesis of CRC cells can be inhibited, cell apoptosis can be induced, and the response to chemotherapy can be enhanced. The application provides a new strategy and direction for the treatment of colorectal cancer, has extremely high clinical application value, and can bring better treatment effect for patients with intestinal cancer.

Anemia, defined as a reduction in the amount of functional red blood cells in circulation, is a major cause of disease affecting one-third of the world's population. Iron is essential for hemoglobin, the functional component of red blood cells, to store and transport oxygen. Hepcidin, a liver-derived peptide, is a key regulator of iron homeostasis. During anemia, the erythropoietic hormone erythroferon regulates hepcidin synthesis to ensure an adequate supply of iron to the bone marrow for red blood cell synthesis. However, accumulating evidence suggests that other factors may perform a similar function. We identified the hepatokine FGL1 as a previously undescribed hepcidin suppressor that is highly induced in the liver in response to hypoxia during recovery from anemia and in thalassemia mice. We demonstrated that FGL1 is a potent hepcidin suppressor in vitro and in vivo. Deletion of Fgl1 in mice blunts hepcidin suppression after hemorrhage.Finally, FGL1 is a BMP antagonist that directly binds to BMP6 and impairs the BMP-SMAD signaling cascade that controls hepcidin regulation.Therefore, the present invention relates to an FGL1 polypeptide for use in treating patients suffering from iron deficiency-related diseases.

The application discloses a kind of fluorescent probe devices with high sensitivity detection in the field of biological fluorescence detection, including microfluidic chip, closed microfluid channel for test sample flow is equipped inside microfluidic chip, microfluid channel includes reaction bin, sample bin and enzyme liquid bin, sample bin and enzyme liquid bin are symmetrically distributed on the both sides of reaction bin and are connected with the delay channel of zigzag structure between reaction bin, first filling opening and second filling opening are respectively opened in sample bin and enzyme liquid bin, reaction bin is opened with the vent hole being communicated with outside, and a plurality of aptamer fluorescent probes are fixed on the bottom wall of reaction bin.The application fixes nucleic acid aptamer fluorescent probe in microfluidic chip, and adds CRISPR-Cas12a enzyme cutting system, releases multiple signal fragments in the process of single aptamer binding antibiotic, realizes signal cascade amplification of CRISPR-Cas12a non-specific enzyme cutting activation, and has the function of detecting the existence of drug-resistant gene, to minimize the possibility of false negative detection results.