40results about How to "Rapid and reliable detection" patented technology

This invention describes a device consisting of a micro channel plate, filter, and porous holder for filter, which is substituted by a pure agar block during method performance, and supportive structural elements. The device is intended for rapid detection and / or identification of microorganisms. Microorganisms are trapped by filtration in long (diameter / length=1 / 10-1 / 100), cylindrical, parallel, micro-channels that are open from both sides and attached to a filter from one side. A micro channel plate houses a multiplicity of micro channels (possible diameter of each channel=1-30 μm, length 100-1000 μm, and number on centimeter2−100,000-1,000,000). The micro channel plate with cells trapped on the surface of the filter is attached to an agar block impregnated by artificial substrate(s) so that the molecules of the artificial substrates will fill all micro channels. Trapped cells produce colored or fluorescent molecules from artificial substrates. These molecules are collected in the very small volume of a micro channel. The extremely small volume of a micro channel (1 / 25 million part of milliliter) allows it to collect a detectable concentration of color or fluorescent substances in a very short time (several minutes). Even one cell from a filtrated sample can be detected by the enzyme—artificial substrate method and / or identified by enzyme immunoassay.

System for control of ion species behavior in a time-varying filter field of an ion mobility-based spectrometer to improve species identification for explosives detection.

An apparatus for delivering energy, and in particular laser energy, to a tissue is adapted to minimize or eliminate burn back caused by contact between the energy delivery apparatus and bodily fluids by (I) preventing the energy delivery apparatus from contacting bodily fluids or tissues that might burn or cause the apparatus to burn; and / or (ii) monitoring the apparatus to detect overheating in order to withdraw the apparatus or control the energy supply in case overheating is detected. The apparatus is applicable, by way of example, to treatment of blood vessels using endovascular techniques.

The present invention relates to a method for the rapid and reliable detection of drug-selected mutations in the HIV protease gene allowing the simultaneous charaterization of a range of codons involved in drug resistance using specific sets of probes optimized to function together in a reverse-hybridization assay. More particularly, the present invention relates to a method for determining the susceptibility to antiviral drugs of HIV viruses in a biological sample, with said method comprising: a) if need be, releasing, isolating or concentrating the polynucleic acids present in the sample; b) if need be amplifying the relevant part of the protease gene of HIV with at least one suitable primer pair; c) hybrydizing the polynucleic acids of step a) or b) with at least one of the following probes: probes specifically hybridizing to a target sequence comprising codon 30; probes specifically hybridizing to a target sequence comprising codon 46 and / or 48; probes specifically hybridizing to a target sequence comprising codon 50; probes specifically hybridizing to a target sequence comprising codon 54; probes specifically hybridizing to a target sequence comprising codon 82 and / or 84; probes specifically hybridizing to a target sequence comprising codon 90; or the complement of said probes; further characterized in that said probes specifically hybridize to any of the target sequences presented in FIG. (1), or the complement of said target sequences; d) inferring from the result of step c) whether or not a mutation giving rise to drug resistance is present in any of said target sequences.

A binding partner, especially an antibody fragment that specifically recognizes an antigen-antibody immune complex between anti-THC and THC (tetrahydrocannabinol), is disclosed. The binding partner facilitates a non-competitive homogenous immunoassay for detection of cannabis use. A test kit comprising the binding partner is also described. Preferably the immunoassay is applied for roadside testing of saliva from suspected drivers.

With a scanning probe microscope, if a plurality of sample properties are measured using a scanning scheme of allowing a probe to approach and withdraw from a sample, the sample properties need to be accurately and reliably detected in the minimum required measurement time. Further, the acting force between the probe and the sample varies depending on the type of the probe and the wear condition of a probe tip. Thus, disadvantageously, property values acquired using different probes cannot be compared with one another unless the artifactual effect of the measuring probes are eliminated. In accordance with the present invention, with a scanning probe microscope, the probe is brought into intermittent contact with the sample, while driving means repeatedly allows the probe to approach and withdraw from the sample with a variable amplitude. The sample property is thus acquired at a high speed. Further, a calibration sample is used in a given environment (given temperature and humidity) to acquire a force curve for at least one point. Information obtained from the force curve is used to correct measurements to display the distribution of the sample property.

The present invention relates generally to the field of Hepatitis B variants exhibiting a reduced sensitivity to nucleoside analogues both in vivo and in vitro. More in particular, reverse transcriptase mutant rt I233V is provided. Present invention provides assays and methods for detecting such variant, which assays are useful in monitoring anti-viral therapeutic regimes and adjusting patient therapy. A diagnostic kit for detecting the presence of an HBV variant in a biological sample has also been described. Finally, the use of a farmaceutical composition to cure a subject suffering from a HBV infection, which HBV is resistant to lamuvidine and / or adefovir has been provided, which farmaceutical composition comprises the nucleoside analogue tenofovir.

The present invention relates generally to the field of Hepatitis B variants exhibiting a reduced sensitivity to nucleoside analogues, both in vivo and in vitro. More in particular, reverse transcriptase mutant rtA181S is provided. Present invention provides assays and methods for detecting such variant, which assays are useful in monitoring anti-viral therapeutic regimes and adjusting patient therapy. A diagnostic kit for detecting the presence of an HBV variant in a biological sample has also been described.

Scanning interferometer and method of using same providing for rapid, reliable detection of chemical compounds that are readily implemented in low-cost, portable configurations for application in a variety of monitoring and detection applications. A scanning double-beam interferometer, particularly a Michelson interferometer, in which the length of at least one of the optical paths (or arms) of the interferometer is selectively adjustable by use of an actuator in which rotational displacement of a rotatable element is converted into linear displacement of at least one reflective surface which forms an end of an optical path of the interferometer is employed to obtain interferograms of electromagnetic radiation attenuated, emitted, scattered or reflected from a sample. The length of the optical path that is adjusted is determined using an optical detection scheme, particularly where marking on the rotatable element are detected to determine linear displacement of the reflective surface.

An optochemical sensor for measuring concentrations of analytes is provided with a reactive matrix preferably made of polymeric material capable of swelling. Further provided are a mirror layer and a layer of a plurality of discrete islands that are electrically conductive, between which layers the reactive matrix is positioned, the diameter of the islands being smaller than the wavelength of the light employed for monitoring and evaluation.

In a method for leak testing in an automated electrohydraulic clutch system in a motor vehicle, in which an electromechanical actuator controls the clutch travel of a clutch via a hydraulic piping system, in order that a defective system is detected reliably and rapidly, the leak in the clutch system is detected using a pressure measurement in the electromechanical actuator and compared to a characteristic clutch curve.

A fully automated system and method for rapidly and reliably detecting the ingestion of slag within the tip of a metal making lance. The system utilizes a temperature sensor disposed adjacent to the inner surface of the inner wall of a metal making lance tip. The sensor is in communication with a control station. The control station is preferably in operative communication with a valve for shutting off gas flow to the lance and, optionally, at least one alarm, a coolant fluid shut-off valve, and any other lance operation mechanisms as may be desired or necessary when slag is detected by the sensor. The inner wall of the lance tip is also preferably formed with a slag collecting reservoir for collecting ingested slag and directing it toward the temperature sensor for quick and reliable slag detection.

An apparatus for delivering energy, and in particular laser energy, to a tissue is adapted to minimize or eliminate burn back caused by contact between the energy delivery apparatus and bodily fluids by (i) preventing the energy delivery apparatus from contacting bodily fluids or tissues that might burn or cause the apparatus to burn; and / or (ii) monitoring the apparatus to detect overheating in order to withdraw the apparatus or control the energy supply in case overheating is detected. The apparatus is applicable, by way of example, to treatment of blood vessels using endovascular techniques.

The purpose of the invention is to provide a dust collection device and a method for detecting fire in the dust collection device, whereby fire can be detected promptly and reliably in the event that a fire has ignited inside the device. A dust collection device (1) that separates dust from dust-containing air, by using a blower to suction in dust-containing air that includes dust, and by causing the dust-containing air to pass through a filter. The dust collection device is characterized by comprising: a dust-containing air chamber (10) to which the suctioned dust-containing air is guided; a clean air chamber (16) separated from the dust-containing air chamber by a separation means including the filter (4), and to which cleaned air filtered by the filter is guided; and a dust detection sensor (24) arranged on the clean air chamber side.

Detection of the failure of a pressure sensor of an air data system of an aircraft having at least two pressure sensors includes measuring first and second pressure values with the aid of the two pressure sensors, respectively. The deviation between the first and second pressure values is calculated, and this deviation is compared with a predetermined threshold value. If the deviation is greater than the threshold value, a failure cue relating to the air data system is displayed on a display device that is mounted in the aircraft.

The present invention relates to new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region, between the 16S and 23S rRNA genes, to be used for the specific detection and / or identification of Serratia species, in particular of Serratia marcescens, Serratia ficaria and / or Serratia fonticola, in a biological sample. The present invention relates also to a method for the specific detection and / or identification of Serratia species, in particular Serratia marcescens, Serratia ficaria and / or Serratia fonticola, using said new nucleic acid sequences derived from the ITS region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Serratia species in a sample.

The invention relates to a method and an apparatus for measuring a rotational speed, in particular a rotational speed of a wheel of a motor vehicle, by means of a speed sensor, wherein the speed sensor produces an output signal characterizing the actual rotational speed and wherein the output signal of the speed sensor is sampled and further processed in accordance with sampled values thus obtained. For an—in hardware terms—simple realization, according to the invention it is provided that the output signal is sampled at a predetermined sampling rate over at least one measuring cycle, that in each case the amplitude of sampled values obtained in the measuring cycle is determined and that from the rate of occurrence of sampled values of an amplitude lying within a predetermined amplitude range at least one signal level occurring during the measuring cycle in the output signal is determined.

A method for representing the surroundings of a vehicle. The method includes: detecting the surroundings of the vehicle and ascertaining obstacles in the surroundings with the aid of surroundings sensors of the vehicle; displaying a first depiction encompassing a representation of the surroundings including the obstacles and a representation of the vehicle on a display device; establishing an approach by the vehicle to an ascertained obstacle due to the fact that a predefined distance to the obstacle has been undershot; and displaying a second depiction including a detail, enlarged in comparison to the first depiction, of the representation of the surroundings including the obstacles and the representation of the vehicle on the display device, the detail representing the area of the surroundings and of the vehicle at which the approach by the vehicle to the obstacle was established.

A method for detecting a water ingress into an interior of a housing, which is sealed against the surroundings, including the following steps: detecting a temperature of the air in the interior; detecting a relative air humidity in the interior; and detecting a water ingress as a function of the detected temperature and of the detected relative air humidity.