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Clenbterol hydrochloride enzyme immunoassay kit and its immnoassay method

A technology of clenbuterol hydrochloride and a detection kit, which is applied in the field of enzyme immunoassay, can solve the problems of not forming a kit and being unable to be popularized and applied, and achieves the effect of simple sample pretreatment

Inactive Publication Date: 2003-01-08
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, both Randox Laboratories Ltd in the UK and rbiopham in Germany have developed ELISA kits for the detection of CLB residues in meat and feed. However, China’s research on the application of EIA to detect CLB started relatively late, and my country is currently applying it in import and export quarantine. China's β-doping agent detection kits are completely dependent on imports. Although the research on ELISA detection has been carried out in China, the kits have not yet been formed, so they cannot be popularized and applied.

Method used

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  • Clenbterol hydrochloride enzyme immunoassay kit and its immnoassay method
  • Clenbterol hydrochloride enzyme immunoassay kit and its immnoassay method

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Embodiment 1

[0038] The monoclonal CLB antiserum prepared according to the aforementioned method was coated on the microtiter plate and placed in a 4°C refrigerator overnight.

[0039] 1. According to the above method, carry out sample processing.

[0040] 2. Reagent preparation.

[0041]1) Standard CLB reagent: add 20ml of B reagent (diluent) to CLB and mix well.

[0042] 2) Enzyme-labeled antigen reagent: Dissolve C reagent plus 10ml D reagent (enzyme-labeled antigen diluent), and mix well.

[0043] 3) Preparation of washing liquid E: add 200 ml of distilled water to E reagent containing phosphate (PBS) of 0.1% Tween-20 (Tween-20) to prepare washing liquid E, which is used for washing the microtiter plate.

[0044] 4) Substrate mixture preparation: according to the required amount each time, use F reagent (o-phenylenediamine) and G reagent (sodium acetate-citric acid buffer) in the ratio of G:F=7-9:3-1 Dilute reagent F, and then add 14 μl of hydrogen peroxide reagent H to each ml of t...

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Abstract

This invention relates to hydrochloric acid CLB enzyme immunity test reagent box and its test method adopting ELISA competition to test CLB and using CLB and OA coupler as CLB antigen immunity rabbits to small mouses to get multiclonal containing CLB antibody or monoclonal CLB antiserum then to be put on enzyme specimen board after separation, purification and dilution to be warm cultivated, washed, added with sample diluent solution and enzyme specimen antigen to be reacted, washed and added with enzyme substrate to display color. It is an easy test method with simple pretreatment especially for testing remaining CLB of meat food.

Description

technical field [0001] A clenbuterol hydrochloride (CLB) enzyme immunoassay kit and a detection method thereof are used for detecting the content of clenbuterol hydrochloride (CLB) in animal source foods, especially pork products. The invention belongs to the technical field of enzyme immunoassay. Background technique [0002] Clenbuterol hydrochloride, commonly known as "Clenbuterol", is a type of β-stimulant. The chemical name is 2-[(tert-butylamino)methyl]-4-amino-3,5-dichlorobenzyl alcohol hydrochloride. The name of the drug is "Ketansu". CLB was first used as an antiasthma drug. At the end of the 1970s, developed countries such as the United States began practical research on the use of CLB as a "nutrient redistribution agent" and "growth promoter". It was widely used in livestock and poultry production in the 1980s. In recent years, animal products The hazards of CLB residues to human health and the damage to the ecological food chain have attracted great attention...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/535G01N33/543G01N33/577
Inventor 胥传来李相前邵蔚蓝徐保国丁宏标詹斌
Owner JIANGNAN UNIV
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