67 results about "Microchip Analysis" patented technology

The invention provides a microchip analysis system integrated capillary electrophoresis separation with chemiluminescence detection, which consists of a microfluidic chip, a single-path high voltage power source, a minipump, a three-way valve, an interface, a needle adjusting valve, a U-tube type monometer, a photomultiplier, a vacuum flask, an electric contact vacuum gauge and a time relay. The microchip analysis system provided by the invention has simple structure, no mutual interference of the capillary electrophoresis separation and chemiluminescence detection, and high separation efficiency of the capillary electrophoresis and high sensitivity of the chemiluminescence, has the characteristics of high separation efficiency, high detection sensitivity, small volume, light weight, convenient operation, low cost and the like, and is an ideal portable microchip analysis system.

A method for setting up a system of a reagent chip analyzer is provided. The method is adapted to modify parameters and testing conditions according to data of an authorization tag, so as to improve the adaptability range of the reagent chip analyzer for reagent chips of multiple specifications. The method includes: reading an identification code, an authorization times, and specifications and analysis parameters of the reagent chip from the RFID tag; determining whether to load the specifications and the analysis parameters of the reagent chip according to the identification code and the authorization times; and if it is determined to load the specifications and the analysis parameters of the reagent chip then decreasing the authorization times by degrees, and analyzing the reagent chip according to the specifications and the analysis parameters of the reagent chip.

This invention relates to one micro flow control chip and its process method with sample pre-process film, wherein, the chip is composed of bottom slice and cover slice; the bottom slice uses glass, silicon, PMMA or PDMS as basic materials to etch flow mixture and reaction micro channel network; the cover slice adopts PDMS process sealed to bottom slice; in the relative position of each channel opening liquid guide hole for micro flow; putting sample process film in the pointing position to sample entrance imbedded into cover slice.

The present provides a method for analyzing a reaction test sample using test sample chips, by which a number of test sample chips can be prepared with fixing test samples of a very slight amount being disposed on each of the test sample chips of substrates at a high density, and detection of reaction test samples can be efficiently carried out with a single operation by using the corresponding test sample chips.

An enzyme immunoassay chip having, as micro channels, a reaction liquid leading-in flow passage part, a reaction flow passage part, and detection flow passage part sequentially disposed on a substrate continuously to each other, comprising an installed part for bead-bodies supporting antibodies and the bead-body flow stopping part formed in the micro channel of the reaction flow passage part, wherein enzyme reactive product flowing beyond the flow stopping part can be analyzed by using the chip.

The present invention relates to the field of biological information testing technology. By using rare earth element ions with unique fluorescent characteristic as marker tracer and through time resolving fluorescent spectroscopic analysis to eliminate the effect of background fluorescence, the sensitivity of chip analysis is raised greatly. The detector of the present invention consists of frame, support platform, platform transmission mechanism, optical detection unit and photoelectronic signal processing unit. The present invention is superior to available technology obviously. The multifunctional instrument combining the temporal fluorescent resolving technology, gene chip detection technology and biological information technology has the advantages of high sensitivity, high stability, wide linearity range and long marker maintaining period.

This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and / or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink™, eSensor™, multiplex arrays with cartridges etc., all described herein.

The invention provides a microfluidic immune chip analysis method based on a biotin and streptavidine system. The method includes the following steps that firstly, a first microfluidic chip is acquired and sealed to a base, coverage substances are introduced into a channel for coverage, and the first chip is removed; secondly, a second microfluidic chip is taken and sealed to the base, and to-be-detected substances are introduced and are washed through buffering liquid after incubation; thirdly, a second antibody marked by biotin or streptavidine is introduced and is washed through a buffering liquid after incubation, and the second antibody is an antibody resistant to the to-be-detected substances; fourthly, HRP marked by streptavidine or biotin is introduced and is washed through a buffering liquid after incubation; fifthly, the second microfluidic chip is removed, HRP is added into a reaction area to catalyze light-emitting optical reaction liquid, and data are read through a chemical light-emitting instrument. The immune analysis method has the advantages that sensitivity is high, the signal reading mode is simple, the detection linearity range is wide, analysis speed is high, detection cost is low, and high-throughout test can be achieved.

The invention relates to the technical field of detection equipment, in particular to a biochip analyzer and an analysis method. The biochip analyzer includes a sample placement device for storing samples, a reagent placement device for storing reagents, a dilution device for diluting the samples in the sample placement device, an incubation and cleaning device for storing biochips, a mechanical arm that moves back and forth between the dilution device and the reagent placement device and successively sucks the diluted samples and the reagents in the reagent placement device for sample application or charging to biochips, a detection device for detection analysis of the incubated and cleaned biochips, and a control device for controlling the reagent placement device, the incubation and cleaning device, the detection device and the mechanical arm. The biochip analyzer and the analysis method provided by the invention employ automatic operation in the whole biochip detection process, and improve the detection efficiency and detection quality.

A microchip analysis device in which the effect of changes in the quantity of light of a discharge lamp of the short arc type can be reduced and measurement results with high reliability can be obtained is achieved in microchip measurement device which has a light source lamp and a lamp operating device, in which the light from the lamp is transmitted by a fill part for the liquid of the microchip to be tested, and in which the quantity of this transmitted light and based on the data obtained the decadic extinction of the liquid to be tested are measured, a device determines the fluctuation of the quantity of light from the light source lamp. Based on data from this device, a control interrupts or stops the detection of the quantity of the light transmitted by the microchip when the fluctuation is outside a preset range.

A biochip to be used for infrared reflection absorption spectroscopy analysis, which is obtainable by forming a metallic layer and an active layer made from a nonmetallic material and has a film thickness less than an infrared wavelength and remarkably flat surface on a solid base and immobilizing a probe on the active layer. A biochip analysis apparatus including the biochip, a liquid passing unit for supplying a sample liquid containing a test substance to the biochip, and an optical system entering infrared light to the biochip and detecting a reflected infrared light. A biochip analysis method using the apparatus.As described above, the novel biochip that is useful for infrared reflection absorption spectroscopy analysis, the biochip analysis apparatus incorporating the biochip, and the biochip analysis method are provided.

The invention discloses a micro-fluidic refractive index detection method used for polymerized triglyceride. By means of the method, the advantage that the efficient space exclusion chromatography is high in flexibility is kept, a detection instrument is effectively simplified and minimized through the micro-fluidic chip analysis technology, the detection device can serve as a portable analysis technical platform to conduct field detection on oil and fat, and the foundation is laid for rapid, flexible and accurate detection technologies and devices of triglyceride polymers in edible oil and fat samples.

The invention provides a chip packaging preprocessing method and a chip analysis method, and belongs to the technical field of integrated circuits. The preprocessing method comprises the following steps of detecting the projection areas of different bare dies on the surface of the multi-chip package; protecting the multi-chip package outside the surface projection area of the bare chip to be removed; corroding the multi-chip package; and stripping the bare die to be removed to obtain an exposed target bare die. The methods are used for FIB pretreatment.

The invention provides a microfluidics immune chip analysis method based on magnetic particle nano-enzyme, and application. The fermentoid activity of the magnetic particle nano-enzyme is used and serves as the substitute goods of HRP (horseradish peroxidase), and the stability of a traditional microfluidics immune chip can be greatly improved. In addition, the method is combined with high-sensitivity chemiluminescent immunoassay, and therefore, detection sensitivity can be greatly improved. Meanwhile, the microfluidics chip has the advantages that a sample amount is small, multiple samples and indexes can be simultaneously and quantitatively detected, and the microfluidics chip is high in specificity and sensitivity, low in price, quick, visual and convenient in operation, a reading result is quantifiable, complex instrument equipment and professional operation personnel are not required, and the microfluidics chip is suitable for quick field detection. Therefore, a powerful tool is provided for detecting micromolecules and biomacromolecules.

The invention is an orthogonal light path type fluorescent detecting device used in microanalytical chip, composed of a light source, an optical system, and a detecting system, and its characteristic lies in that the optical system adopts an orthogonal light path, the laser emitted from the light source reaches the microanalytical chip from the side wall, and the detecting system detects the fluorescent light outgoing from the side wall. The advantages: the optical system is simple, the device is easy to make, the making cost is low, the detecting sensitivity is high, and the system is easily integrated and miniaturized.

The invention discloses a photon encoding microsphere-based multielement biomarker assay kit-type chip. The photon encoding microsphere-based multielement biomarker assay kit-type chip comprises a chip main body at an upper part and a substrate at a lower part. The interior of the chip main body is etched with multiple independent detection units. Each one of the detection units comprises an exhaust vent, a reaction cabin, a channel and a sampling hole. The sampling hole is communicated with the reaction cabin by the channel. The exhaust vent communicated with environment outside the chip main body is arranged in the reaction cabin. Microspheres as detection carriers are enclosed in the reaction cabin and biological molecular probes are fixed on surfaces of the microspheres. According to the invention, a photonic crystal liquid phase chip technology as a base and a self-developed PDMS kit-type chip are combined so that operation difficulty of a photonic crystal liquid phase chip analysis technology is effectively reduced and an operation process is standardized and later-stage automatic detection development is promoted. The detection method adopting the photon encoding microsphere-based multielement biomarker assay kit-type chip is simple and efficient and can replace the existing clinical analysis technology.

The invention discloses a high-throughput micro-fluidic LAMP chip for detecting livestock respiratory tract pathogens and a detection method, and belongs to the technical field of biochips. The LAMP chip comprises an LAMP amplification reagent, a DNA template, an LAMP quality control positive control, an LAMP quality control negative control, a detection chip, a chip probe and a microfluidic LAMPchip analyzer, wherein the detection chip is arranged in the isothermal amplification microfluidic LAMP chip analyzer; and the chip probe comprises nucleotide sequences as shown in SEQ ID NO: 1-23. The LAMP chip is used for detecting specific nucleic acid fragments of mannheimia haemolytica, pasteurella multocida, mycoplasma, arcanobacterium pyogenes and klebsiella pneumoniae. The LAMP chip provided by the invention can complete detection within 50 minutes, has a detection limit of 1.5x10<-2> ng / mu L, can monitor main livestock respiratory tract pathogens in real time, has the characteristicsof simple operation, rapid detection, high sensitivity and strong specificity, and brings convenience to detection of the livestock respiratory tract pathogens.

An enzyme immunoassay chip having, as micro channels, a reaction liquid leading-in flow passage part, a reaction flow passage part, and detection flow passage part sequentially disposed on a substrate continuously to each other, comprising an installed part for bead-bodies supporting antibodies and the bead-body flow stopping part formed in the micro channel of the reaction flow passage part, wherein enzyme reactive product flowing beyond the flow stopping part can be analyzed by using the chip.

The invention discloses an automatic student height and weight analyzing system based on an ultrasonic technology. Identity information is identified through an identity identification module in a wristband, the human length is measured through an ultrasonic ranging module in the wristband, and daily increase amount is compared; the weight of a student is measured by a weighting sensor, the increase amount is compared, data is sent to an MCU chip through a communication device, BMI data is analyzed, the weight-height ratio is measured in real time, and nutrient supplementation is prompted. Theautomatic student height and weight analyzing system has the advantages that wristband part change is convenient and quick, the wristband does not likely drop or break, measurement is accurate, big data analysis results are quick and timely, and body condition can be informed and monitored in time.

The invention relates to a human glomerular endothelial cell culture and drug detection toxicity evaluation method based on a micro-fluidic chip, and the specific culture method comprises the following steps: carrying out cell culture and test by using a micro-fluidic cell chip analyzer, and specifically comprises the following steps: 1, preprocessing the chip; step 2, cell inoculation; step 3, cell culture; after cell culture is completed, successfully cultured cells are used for subsequent drug evaluation detection, and the method specifically comprises the following steps: step 1, preparation for detection; 2, detecting the concentration of LDH; step 3, activity detection; in addition, living and dead cell staining, immunofluorescence detection and inflammatory cytokine detection can also be used, the temperature and gas components of a cell growth area can be accurately regulated and controlled by implementing the steps, an external incubator is omitted, long-term continuous observation of cells under a microscope is achieved, and the detection accuracy is improved. The microfluidic cell model can better simulate the human body microenvironment, and the drug evaluation effect is more accurate.

Microfluidic systems, pumps, valves and applications of the same are provided. The microfluidic system may be a pump or a valve having a fluidic chip and an actuator controlling the opening and closing of the fluidic channel in the fluidic chip. The actuator may be disposed to tilt from the fluidic chip, forming a tilted-rotor peristaltic pump. Alternatively, the actuator may be a rolling ball actuator, and different fluidic chips may be used in different applications. For example, the fluidic chip may be a spiral pump chip having spiral channels, a rotary peristaltic pump chip having multiple output channels, or a multi-port valve chip having one port interconnected with multiple different ports. An analytical valve chip may switchably interconnect bioreactor and rinse / calibration input channels to sensor and waste output channels. The actuator of a random-access valve can move from one valve position to another without opening or closing intermediate ones.

The invention discloses a chip analysis system fro chip electrophoretic separation and plasma mass spectrometric detection. With a center through hole being the circle center, at least two identical radial flow-distributing channels are formed on the edge of each micro-fluidic chip in a radiated mode and communicated with the center through hole; a separation channel comprises two micro-fluidic chips arranged symmetrically, the micro-fluidic chips are arranged at the initial end and the terminal end respectively, opposite radial flow-distributing channels are communicated through connecting capillary tubes, and porous plugs are evenly arranged inside the radial flow-distributing channels of the micro-fluidic chip at the initial end; the center through hole of the micro-fluidic chip at the initial end is connected with a high-voltage electrode, the center through hole of the micro-fluidic chip at the terminal end is connected with a grounding electrode, and the high-voltage electrode and the grounding electrode are connected with the two ends of a high-voltage power supply respectively; a transfer capillary tube of a outflow channel is connected with a plasma mass spectrometer through an atomizer. The chip analysis system has the advantages of being high in separation efficiency and detection sensitivity, simple in structure, convenient to operate and low in cost.

The invention provides a biochip analyzer. The biochip analyzer comprises a rack; a PCR amplification device, a chip feeding device and a sample detection device are arranged on the rack; the PCR amplification device comprises a first shell and a heating platform; the heating platform has a retraction position in which the heating platform is located in the first shell and an extension position in which the heating platform is located outside the first shell; and a to-be-detected sample placing area is formed at the top of the first shell. The biochip analyzer further comprises a transfer device; the transfer device can be used for transferring a sample on the heating platform at the extending position to the to-be-detected sample placing area, transferring the sample from the to-be-detected sample placing area to the sample detection device, and transferring a chip in the chip feeding device into the sample detection device. According to the biochip analyzer of the invention, the transfer device and a plurality of functional devices cooperatively act, so that the functions of PCR amplification, sample and biochip feeding, biochip detection and analysis and the like can be realized, the automation degree and the integration degree are high, the analysis and detection efficiency is higher, and the labor cost is greatly reduced.

The invention discloses a construction method of a biosensing system for detecting the physiological and pathological parameters of an organ chip. The method comprises the following steps of injecting a cell mixed solution into a pre-designed chip model, and continuously culturing to form the organ chip; and then connecting an electrochemical sensing chip provided with a micro-flow control pipeline to an outlet of the organ chip for specifically monitoring the oxygen content, ATP, pH, ROS and cholesterol in metabolites of the organ chip. An organ chip analysis system is simple and convenient to construct, is wide in application range and diversified in analysis parameters; the organ chip is combined with an electrochemical sensor, so that the growth micro-environment of a three-dimensional in-vitro blood vessel / heart model can be dynamically monitored in real time. According to the electrochemical biosensing system for monitoring the physiological and pathological indexes of the organ chip, the model culture condition can be adjusted in real time, so that the model culture condition better conforms to the real living body condition, and the electrochemical biosensing system can be used for exploring the physiological state change in the disease process and also can be used for monitoring the response of the organ chip to drugs on line.

The invention relates to the technical field of power grid data analysis, in particular to a power grid big data efficient collection method, which comprises the following steps of S1, setting a powergrid monitoring system in a power grid, and performing voltage conversion on power grid voltage, S2, performing conversion and PT / C T conversion on the three-phase voltage through a voltage and current transformer, and converting the three-phase voltage into a signal suitable for being acquired by an AD chip through sampling and filtering conversion, S3, inputting the obtained signal into a chipfor analysis and processing, S4, directly controlling driving programs of various hardware interfaces to communicate with an instrument system through driving software by utilizing an analysis resultto carry out reverse control, and S5, performing calculation and storage of each parameter in a time domain and a frequency domain on the analyzed and processed power grid data collected by the chip,and performing power grid big data visualization processing to form a visual picture. A big data carrier is provided for a power grid, the management level of safe and economic operation of a power transmission line can be improved, and necessary reference information is provided for state maintenance work of the power transmission line.

The invention relates to the technical field of semiconductor wiring, and discloses a high-density wiring reset method which comprises the following steps: S100, selecting and marking a collapsed or deformed area on a chip as a target area; S200, polishing the target area to expose the lead and the pad in the chip, selecting and marking the damaged wiring as the target wiring and selecting the padto be connected as the target pad; S300: using a crochet to hook off the target wiring under a stereo microscope, pulling out the target wiring on the pad and using crochet equipment to hook off thewiring on the target pad; S400: closing the fracture of the target wiring and the fracture of the wiring on the target pad and adding a connection medium for fixing and electrically connecting the wiring port at the closed place; and S500: sealing the target area. The wiring repair is directly performed on the chip and the analysis speed of the chip can be effectively improved through the wiring repair method without re-framing and wiring analysis from the packaging factory so as to shorten the analysis cycle of the chip.

The invention discloses a self-learning optimization method for optimal parameter selection of a chip module, which is a rapid automatic algorithm and is used for traversing and combining configuration parameters of a chip so as to select a group of external excitation configuration and chip configuration tuning parameters for ensuring the optimal overall performance of the chip. The device is especially suitable for automatic testing of performance verification of a low-power-consumption microcontroller (hereinafter referred to as MCU) chip simulation module sensitive to input simulation parameters and a digital-analog interface module. The method is composed of a data analysis self-learning algorithm and a program control automatic acquisition platform. The data analysis self-learning algorithm adjusts various parameters of the chip to be evaluated through the program-controlled automatic acquisition platform, obtains performance index feedback, obtains working environment ranges of different parameters, and analyzes and obtains optimal parameters after the performance and the working range are balanced; data are updated according to batch chip analysis results, a standard database is established, new chips are selectively scanned according to parameters and a working environment range, the test time is saved, and early warning is provided when a standard curve is deviated.

The invention relates to a microchip analysis system combining non-aqueous electrophoresis with peroxyoxalate ester chemiluminiscence. The traditional analysis system has various problems. The microchip analysis system comprises a micro-flow control chip, a negative pressure device, a direct current power supply and a photomultiplier, wherein the micro-flow control chip comprises a substrate, a buffer liquid storage tank, a sample liquid storage tank, a sample waste liquid tank, a buffer waste liquid tank, a peroxyoxalate ester liquid storage tank, a hydrogen peroxide liquid storage tank, and a waste liquid tank arranged on the substrate; the negative pressure device comprises a vacuum flask, a miniature vacuum pump, an electric contact vacuum meter, a needle valve, a U-shaped tube pressure meter, a two-position three-way electromagnetic valve and a time relay; an output end of the direct current power supply is connected with the buffer liquid storage tank; the grounding end is connected with the buffer waste liquid tank; and the photomultiplier is directly arranged below the detection channel. The microchip analysis system is simple in structure, convenient to operate, good in compatibility, high in separation efficiency, and high in detection sensitivity; and non-aqueous electrophoresis and peroxyoxalate ester chemiluminiscence detection are not interfered by each other.