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Combined device and detection method for synchronously detecting influenza A virus, influenza B virus, chlamydia pneumoniae IgM antibody and mycoplasma IgM antibody

A technology of Chlamydia pneumoniae and Mycoplasma pneumoniae, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to fully analyze and understand the disease, procrastination of the disease, and medication errors, so as to simplify the clinical detection process and improve the efficiency of diagnosis. The effect of rational drug use and convenience

Pending Publication Date: 2020-09-01
北京柏兆嘉业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. The requirements for test samples are not uniform, and multiple tests and diagnoses are required: the current clinical treatment method is to perform rapid differential diagnosis of influenza virus type A / B, Chlamydia pneumoniae (CP) and Mycoplasma pneumoniae at the same time according to clinical symptoms , it is necessary to issue test sheets multiple times, collect different test specimens multiple times, use multiple detection reagents from the same manufacturer or different manufacturers, and perform multi-step complex operations to achieve
[0009] 2. Due to discontinuity and incompleteness between multiple tests and / or diagnoses, resulting in medication errors or delays in the disease: In a single detection of influenza virus type A / B and Chlamydia pneumoniae (CP) / Mycoplasma pneumoniae detection and clinically based on the results When judging, it will lead to the reality that the condition cannot be fully analyzed and understood, and it may be because the patient is too lazy to perform multiple tests repeatedly, which will affect timely treatment

Method used

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  • Combined device and detection method for synchronously detecting influenza A virus, influenza B virus, chlamydia pneumoniae IgM antibody and mycoplasma IgM antibody
  • Combined device and detection method for synchronously detecting influenza A virus, influenza B virus, chlamydia pneumoniae IgM antibody and mycoplasma IgM antibody
  • Combined device and detection method for synchronously detecting influenza A virus, influenza B virus, chlamydia pneumoniae IgM antibody and mycoplasma IgM antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A combined device for synchronous detection of influenza virus type A, type B and IgM antibodies of chlamydia pneumoniae and mycoplasma, its structure is as follows figure 2 As shown, it includes a dual-channel jam; the test strip FluA & B and the test strip CP & MP arranged side by side in the dual-channel jam; the first one independently connected to the test strip FluA & B and the test strip CP & MP that is arranged on the jam Sample hole S1 and the second sample hole S2.

[0043] The specific design of each part is as follows:

[0044] The structure of the test strip FluA&B is as follows image 3 As shown, including the first sample pad 1 pretreated with anti-erythrocyte antibody, the first filter blood pad 2, the FluA & B tracer sprayed glass fiber pad 3, the FluA & B antigen-coated nitrocellulose membrane 4, and the first absorbent paper 5 and the first plastic bottom plate 6; the first sample pad 1, the first blood filter pad 2, the FluA & B tracer sprayed gla...

Embodiment 2

[0051] The preparation method of the combined device of synchronously detecting influenza virus type A, type B and chlamydia pneumoniae and mycoplasma described in embodiment 1 may further comprise the steps:

[0052] a) Prepare tracers: tracers include but are not limited to colloidal gold, colloidal selenium, colored latex microspheres, fluorescent dyes, fluorescent microspheres, quantum dots and carbon black particles, etc.;

[0053] b) using the tracer prepared in step a) to label the anti-human IgM μ chain antibody and the quality control line to label the ligand to obtain the labeled tracer;

[0054] c), using the tracer buffer to dilute the marked tracer in step b) to obtain a tracer solution, and spray the tracer solution on the glass fiber to obtain a tracer sprayed glass fiber mat;

[0055] d) Spray a piece of nitrocellulose membrane with pre-diluted highly specific influenza virus A and B as the influenza virus A detection line and influenza virus B detection line, ...

Embodiment 3

[0060] Detection method and result interpretation

[0061] A method for synchronous detection of influenza virus type A, type B and chlamydia pneumoniae CP, mycoplasma pneumoniae MP, it uses the synchronous detection of influenza virus type A, type B and chlamydia pneumoniae CP, mycoplasma pneumoniae MP in embodiment 1 The combination means of IgM antibody comprises the following steps:

[0062] The first step is to obtain test specimens in accordance with clinical sample collection specifications;

[0063] The second step is to use the supporting sample diluent in combination at the same time, that is, while adding the test specimen to the first sample injection hole S1 and the second sample injection hole S2, add the sample diluent to each sample injection hole, and the sample dilution The amount of solution used is 70-95ul / sample well;

[0064] The third step, after adding the sample, start timing for 10-15 minutes;

[0065] The fourth step, when the timing is over, imme...

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Abstract

The invention belongs to the field of medical detection equipment, and provides a combined device for synchronously detecting IgM antibodies of influenza A and B viruses and chlamydia pneumoniae and mycoplasma pneumoniae. The device comprises a double-channel clamping shell, a test strip FluA & B and a test strip CP & MP which are arranged in the double-channel clamping shell in parallel; influenza virus A detection lines arranged on a nitrocellulose membrane of the test strip FluA & B at intervals are coated with high-specificity influenza virus A antigens, influenza virus B detection linesare coated with high-specificity influenza virus B antigens, and first quality control lines are coated with quality control line coating receptors; chlamydia pneumoniae detection lines (CP) arranged on a nitrocellulose membrane of the test strip CP & MP at intervals are coated with high-specificity chlamydia pneumoniae antigens, mycoplasma pneumoniae detection lines (MP) are coated with high-specificity mycoplasma pneumoniae antigens, and second quality control lines are coated with quality control line coating receptors.

Description

technical field [0001] The present invention generally relates to the technical field of medical detection equipment, in particular to a combined device for synchronous detection of influenza virus type A, type B, chlamydia pneumoniae, and IgM antibody of mycoplasma pneumoniae and a detection method thereof. Background technique [0002] Influenza virus type A / B is the primary causative agent of influenza, producing severe complications in patients with underlying pathology. Among them, influenza virus type A antigens are prone to mutations and have caused worldwide pandemics many times; influenza virus type B is less pathogenic to humans, and its epidemic scale is much smaller than that of type A. Influenza virus is a myxovirus with polymorphism, generally spherical, and some are filamentous or rod-shaped, and the diameter of the virus is 80-120nm. The structure of influenza virus mainly includes inner core, capsid and outer envelope. [0003] The source of infection of i...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56983G01N33/56927G01N33/56933G01N33/558
Inventor 姚洪涛
Owner 北京柏兆嘉业科技有限公司
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