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Hepatitis C virus core antigen chemiluminescence ELISA detection kit

A technology of hepatitis C virus and chemiluminescent enzyme, which is applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of "excessive window period" and low sensitivity of hepatitis C antigen enzyme-linked immunoassay kit, and achieve improvement Specificity and sensitivity, breaking through the limitation of detectable time, and expanding the detection range of antigens

Active Publication Date: 2012-06-27
山东莱博生物科技有限公司
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Problems solved by technology

[0005] In order to avoid the limitations of existing detection methods, the purpose of the present invention is to provide Chemiluminescent enzyme-linked immunosorbent assay kit for hepatitis C virus core antigen

Method used

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  • Hepatitis C virus core antigen chemiluminescence ELISA detection kit
  • Hepatitis C virus core antigen chemiluminescence ELISA detection kit
  • Hepatitis C virus core antigen chemiluminescence ELISA detection kit

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Embodiment Construction

[0036] The hepatitis C virus core antigen chemiluminescence ELISA kit of the present invention comprises:

[0037] 96 servings or 48 servings of anti-HCV-cAg monoclonal antibody coated chemiluminescent plate 1 piece, anti-HCV-cAg enzyme conjugate, 6 bottles of standard products, HCV-cAg positive control, HCV-cAg negative control, pretreatment solution , 1 bottle of HCV-cAg sample diluent, chemiluminescence substrate solution A, chemiluminescence substrate solution B, washing solution (20×concentrated), 1 bottle each, 1 instruction manual, 2 self-adhesive paper sheets.

[0038] The preparation method and practical application of the hepatitis C virus core antigen chemiluminescent ELISA kit of the present invention:

[0039] 1. Coating anti-HCV-cAg monoclonal antibodies Mab1 and Mab2 to become a chemiluminescence reaction plate.

[0040] ELISA plate is milky white opaque polystyrene 96-well chemiluminescent ELISA plate.

[0041] The optimal preparation condition of the antibody ...

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Abstract

The invention discloses a reagent kit for carrying out chemiluminescent enzyme-linked immunodetection on a hepatitis C virus core antigen. The reagent kit comprises a kit body, and an enzyme label plate, a reagent, a non-drying glue sealing strip and an operating instruction manual which are arranged inside the kit body, wherein the enzyme label plate is a chemiluminescent enzyme label plate; each hole of the plate is coated with coated antibodies Mab1and Mab2 with concentration of 5ug / ml; and the reagent comprises a HCV-cAg antienzyme composition, a gradient solution of a HCV core antigen standard product, positive control serum, negative control serum, a pretreatment solution, a diluent of a HCV-cAg sample, a chemiluminescent zymolyte solution A, a chemiluminescent zymolyte solution B and a 20 * concentrated cleaning solution. The positive detection rate of the reagent kit is close to that of PCR; and simultaneously, the reagent kit has the advantages of simple operation, high sensitivity and high specificity, and is suitable for popularization and application in clinic.

Description

technical field [0001] The invention relates to a virus antigen detection kit, in particular to a hepatitis C virus core antigen (HCV-coreAg) chemiluminescence ELISA detection kit and a preparation method of the kit. Background technique [0002] Hepatitis C is caused by hepatitis C virus (HCV) infection and is mainly transmitted through blood. There are 170 million people infected with HCV in the world, and it is estimated that there are 38 million HCV infected people in my country, and the number of new cases is rising every year. Compared with hepatitis B, hepatitis C is more likely to become chronic. About 70% of hepatitis C may develop into chronic hepatitis, 20% develop into liver cirrhosis, and 12% develop into liver cancer. The harm is very serious. At present, there is no other effective treatment for chronic hepatitis C except for the definite curative effect of interferon, and it is difficult to make a breakthrough in the development of vaccines in the short term...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/547G01N33/576
Inventor 朱之炜盖中涛卢志明宿振国郭琴燕
Owner 山东莱博生物科技有限公司
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