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Reagents For HCV Antigen-Antibody Combination Assays

a technology of antigen and combination assays, applied in the field of immunoassays for detection and diagnosis of hcv infection, can solve the problem that the first generation of hcv core antigen assays cannot detect core antigens

Inactive Publication Date: 2010-11-25
ORTHO-CLINICAL DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006]The uniqueness of the combination immunoassays of the present invention resides principally in the use of a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without any pre-processing of the sample, yet does not interfere with the capture of anti-HCV antibodies by recombinant HCV antigens, thereby permitting simultaneous measurement of the HCV core antigen and anti-HCV antibodies.

Problems solved by technology

Furthermore, the first generation HCV core antigen assay is unable to detect core antigen when the antigen forms immune-complexes with anti-core antibodies in the late seroconversion phase.
However, one of the challenges to the successful antigen antibody combined assay is to select an appropriate detergent to disrupt HCV virions and release antigen without interfering with the capture of anti-HCV antibodies by recombinant HCV antigens.

Method used

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  • Reagents For HCV Antigen-Antibody Combination Assays

Examples

Experimental program
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Effect test

example 1

Anti-HCV Antibody Detection (an Indirect Assay Format)

[0050]The assays were performed using reagents from an Ortho HCV 3.0 ELISA kit. 150 μL specimen diluents composed of a phosphate based buffer containing detergent Tween-20, BSA, casein, yeast extracts and other proteins, plus 50 μL specimen were added to each well. The plates were incubated at 37° C. for 60 minutes, and were then washed 5 times with a PBS / Tween20-containing wash buffer. Afterwards 200 μL HRP conjugated murine monoclonal anti-human IgG antibody was added to each well. The plates were incubated at 37° C. for 60 minutes and subsequently washed 6 times. 200 μL OPD / substrate (one OPD tablet (Sigma, Cat #P-8287) in 6 mL of ELISA substrate buffer) was added to each well and the plates were incubated in the dark at room temperature for 30 minutes. A 4N H2SO4 stop solution was added at 50 μL / well. ODs were recorded at 493 nm using a plate spectrophotometry reader. Assay cut-off setting was adapted from HCV 3.0 ELISA that ...

example 2

Anti-HCV Antibody Detection (Ag Sandwich Assay Format)

[0054]In the Ag sandwich assay format, antigens labeled with biotin were used as conjugate. The biotin-antigen conjugates bound to human anti-HCV antibodies that were captured on solid phase to form an Ag-Ab-Ag sandwich. The sandwiched complexes were then detected by HRP conjugated streptavidin in a subsequent incubation.

[0055]COSTA™ high-binding microtiter plates were coated with 200 μL / well of premixed monoclonal antibodies C11-3 and C11-7 at 2.2 μg / mL of each and HCC5N-BSA at 0.1 μg / mL in 20 mM phosphate buffer (pH 7.0). The plates were incubated at 25° C. overnight. The coating solutions were aspirated and 200 μl / well of rNS3(h) at 2 μg / mL in PB (pH7.0) was added. Plates were incubated at 25° C. overnight. The plates were then washed one time with PBS / Tween wash buffer, followed by addition of 300 μL / well PBS / BSA blocking solution (1% bovine serum albumin and 30% sucrose in PBS) for 1 hour at 25° C. The plates were aspirated ...

example 3

HCV Core Antigen Detection

[0057]The HCV core antigen detection shown in Example 3 was a monoclonal antibody-HCV core antigen-monoclonal antibody sandwich ELISA. The exposed HCV core antigen in specimen was captured by two monoclonal anti-HCV core antibodies coated on plates and detected by another two HRP labeled monoclonal anti-HCV antibodies.

[0058]Except specimen diluent, ELISA reagents used in Example 3 were basically from the Ortho HCV Core Antigen ELISA kit. “ELISA-7” used the original Kit specimen diluent that contained 1.0% detergent N-Lauroylsarcosine (NLS). However, “ELISA-8” used specimen diluent containing 1.0% detergent Tween 20 and “ELISA-9” used specimen diluent containing 1.0% detergent Lauryldimethylamine N-oxide (LDAO).

[0059]ELISA was performed following HCV Core Antigen ELISA's protocol. 100 μL specimen diluent and 100 μL specimen were added to each well. The specimen incubation was performed at 37° C. for 90 minutes with shake. The plate was washed 5 times with PB...

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Abstract

The present invention is directed to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample. The combination immunoassays of the present invention employ a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without interfering with the performance of other reagents such as the capture of anti-HCV antibodies by recombinant HCV antigens.

Description

FIELD OF THE INVENTION[0001]The present invention generally relates to immunoassays for detection and diagnosis of HCV infection. In particular, the present invention relates to combination immunoassays, reagents and kits based on use of a non-ionic detergent for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample.BACKGROUND OF THE INVENTION[0002]The hepatitis C virus (HCV), a single-stranded RNA virus, is the etiological agent of blood-borne non-A, non-B hepatitis. Chronic active infection with HCV often progresses to liver cirrhosis and hepatocellular carcinoma. Epidemiological studies indicate that HCV infects more than 170 million people worldwide with a high incidence of chronic disease ultimately progressing (more than 50% of cases) to death. However, since it is mainly a blood-borne disease, it is possible to identify the pathogen in blood samples and to eliminate the transmission of disease through blood transfusion. After exposure to the HCV pathogen,...

Claims

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Application Information

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IPC IPC(8): C12Q1/70
CPCG01N2333/186G01N33/5767G01N33/54386G01N33/56983G01N33/5306G01N2469/10G01N2469/20
Inventor ZHENG, JIANYANG, JIANPING
Owner ORTHO-CLINICAL DIAGNOSTICS
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