Hepatitis C virus core antigen chemiluminescence ELISA detection kit

A technology of hepatitis C virus and chemiluminescent enzyme, which is applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of "excessive window period" and low sensitivity of hepatitis C antigen enzyme-linked immunoassay kit, and improve Specificity and sensitivity, breaking through the limitation of detectable time, and expanding the detection range of antigens

CN101419238AActive Publication Date: 2009-04-29山东莱博生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2009-04-29

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Abstract

The invention discloses a reagent kit for carrying out chemiluminescent enzyme-linked immunodetection on a hepatitis C virus core antigen. The reagent kit comprises a kit body, and an enzyme label plate, a reagent, a non-drying glue sealing strip and an operating instruction manual which are arranged inside the kit body, wherein the enzyme label plate is a chemiluminescent enzyme label plate; each hole of the plate is coated with coated antibodies Mab1and Mab2 with concentration of 5ug / ml; and the reagent comprises a HCV-cAg antienzyme composition, a gradient solution of a HCV core antigen standard product, positive control serum, negative control serum, a pretreatment solution, a diluent of a HCV-cAg sample, a chemiluminescent zymolyte solution A, a chemiluminescent zymolyte solution B and a 20 * concentrated cleaning solution. The positive detection rate of the reagent kit is close to that of PCR; and simultaneously, the reagent kit has the advantages of simple operation, high sensitivity and high specificity, and is suitable for popularization and application in clinic.
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Description

Technical field

[0001] The invention relates to a virus antigen detection kit, in particular to a hepatitis C virus core antigen (HCV-coreAg) chemiluminescence enzyme-linked immunoassay kit and a preparation method of the kit. Background technique

[0002] Hepatitis C is caused by hepatitis C virus (HCV) infection and is mainly spread through blood. 170 million people worldwide are infected with HCV, and 38 million people are expected to be infected with HCV in my country, and the number of new cases is increasing every year. Compared with hepatitis B, hepatitis C is more likely to become chronic. About 70% of hepatitis C may develop into chronic hepatitis, 20% may develop into cirrhosis, and 12% may develop into liver cancer, which is very serious. At present, apart from the definite curative effect of interferon, there is no other effective treatment for chronic hepatitis C, and it is difficult to make breakthrough progress in vaccine development in a short time. Therefore, ear...

Examples

Embodiment Construction

[0036] The hepatitis C virus core antigen chemiluminescence enzyme-linked immunoassay kit of the present invention includes:

[0037]96 people or 48 people anti-HCV-cAg monoclonal antibody coated chemiluminescence plate, anti-HCV-cAg enzyme conjugate, 6 bottles of standard products, HCV-cAg positive control, HCV-cAg negative control, pretreatment solution , HCV-cAg sample diluent, chemiluminescence substrate solution A, chemiluminescence substrate solution B, washing solution (20×concentrated) each 1 bottle, a manual, 2 stickers.

[0038] The preparation method and practical application of the hepatitis C virus core antigen chemiluminescence enzyme-linked immunosorbent assay kit of the present invention:

[0039] 1. Coated with anti-HCV-cAg monoclonal antibodies Mab1 and Mab2 to become a chemiluminescence reaction plate.

[0040] Choose milky white opaque polystyrene 96-well chemiluminescence microtiter plate for the microtiter plate.

[0041] The best preparation conditions for t...