Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hepatitis C virus core antigen chemiluminescence ELISA detection kit

A technology of hepatitis C virus and chemiluminescent enzyme, which is applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of "excessive window period" and low sensitivity of hepatitis C antigen enzyme-linked immunoassay kit, and improve Specificity and sensitivity, breaking through the limitation of detectable time, and expanding the detection range of antigens

Active Publication Date: 2009-04-29
山东莱博生物科技有限公司
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to avoid the limitations of existing detection methods, the purpose of the present invention is to provide Chemiluminescent enzyme-linked immunosorbent assay kit for hepatitis C virus core antigen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hepatitis C virus core antigen chemiluminescence ELISA detection kit
  • Hepatitis C virus core antigen chemiluminescence ELISA detection kit
  • Hepatitis C virus core antigen chemiluminescence ELISA detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The hepatitis C virus core antigen chemiluminescence enzyme-linked immunoassay kit of the present invention includes:

[0037]96 people or 48 people anti-HCV-cAg monoclonal antibody coated chemiluminescence plate, anti-HCV-cAg enzyme conjugate, 6 bottles of standard products, HCV-cAg positive control, HCV-cAg negative control, pretreatment solution , HCV-cAg sample diluent, chemiluminescence substrate solution A, chemiluminescence substrate solution B, washing solution (20×concentrated) each 1 bottle, a manual, 2 stickers.

[0038] The preparation method and practical application of the hepatitis C virus core antigen chemiluminescence enzyme-linked immunosorbent assay kit of the present invention:

[0039] 1. Coated with anti-HCV-cAg monoclonal antibodies Mab1 and Mab2 to become a chemiluminescence reaction plate.

[0040] Choose milky white opaque polystyrene 96-well chemiluminescence microtiter plate for the microtiter plate.

[0041] The best preparation conditions for t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a reagent kit for carrying out chemiluminescent enzyme-linked immunodetection on a hepatitis C virus core antigen. The reagent kit comprises a kit body, and an enzyme label plate, a reagent, a non-drying glue sealing strip and an operating instruction manual which are arranged inside the kit body, wherein the enzyme label plate is a chemiluminescent enzyme label plate; each hole of the plate is coated with coated antibodies Mab1and Mab2 with concentration of 5ug / ml; and the reagent comprises a HCV-cAg antienzyme composition, a gradient solution of a HCV core antigen standard product, positive control serum, negative control serum, a pretreatment solution, a diluent of a HCV-cAg sample, a chemiluminescent zymolyte solution A, a chemiluminescent zymolyte solution B and a 20 * concentrated cleaning solution. The positive detection rate of the reagent kit is close to that of PCR; and simultaneously, the reagent kit has the advantages of simple operation, high sensitivity and high specificity, and is suitable for popularization and application in clinic.

Description

Technical field [0001] The invention relates to a virus antigen detection kit, in particular to a hepatitis C virus core antigen (HCV-coreAg) chemiluminescence enzyme-linked immunoassay kit and a preparation method of the kit. Background technique [0002] Hepatitis C is caused by hepatitis C virus (HCV) infection and is mainly spread through blood. 170 million people worldwide are infected with HCV, and 38 million people are expected to be infected with HCV in my country, and the number of new cases is increasing every year. Compared with hepatitis B, hepatitis C is more likely to become chronic. About 70% of hepatitis C may develop into chronic hepatitis, 20% may develop into cirrhosis, and 12% may develop into liver cancer, which is very serious. At present, apart from the definite curative effect of interferon, there is no other effective treatment for chronic hepatitis C, and it is difficult to make breakthrough progress in vaccine development in a short time. Therefore, ear...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/576G01N33/547
Inventor 朱之炜盖中涛卢志明宿振国郭琴燕
Owner 山东莱博生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com