Virus disaggregating agent and method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen

An antigen-antibody and complex technology, which is applied in the preparation methods of peptides, chemical instruments and methods, antiviral immunoglobulins, etc., can solve the problems of affecting antigen detection, low detection rate, and difficulty in detection, and achieves easy operation. , the effect of improved sensitivity and low cost

Active Publication Date: 2013-08-21
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In practical application, since the free core antigen in the serum is very small, and the core antigen is wrapped by the envelope protein, at the same time, the serum may contain one or more different antigens, and some antigens are not easy to detect because the content is too low , thus affecting the detection of antigens, which is an important reason for the low detection rate and low positive rate of the existing antigen detection methods

Method used

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  • Virus disaggregating agent and method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen
  • Virus disaggregating agent and method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen
  • Virus disaggregating agent and method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen

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Experimental program
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Effect test

Embodiment 1

[0055] Each 100ml of the lysing agent contains: NP40: 0.1ml, dimercaptoethanol: 0.01ml, chloroform: 0.1ml, 99.79ml of glycine hydrochloride buffer with pH of 2.8 and concentration of 0.1M / L.

Embodiment 2

[0057] Each 100ml of the lysing agent contains: CHAPS: 0.5ml, urea: 20g, dimercaptoethanol: 0.5ml, chloroform: 0.5ml, pH 2.8, 88.5ml of glycine hydrochloride buffer with a concentration of 0.1M / L.

Embodiment 3

[0059] Each 100ml of lysing agent contains: NP40: 1ml, urea: 10g, dimercaptoethanol: 1ml, acetone: 1ml, pH 2.8, 92ml of glycine hydrochloride buffer with a concentration of 0.5M / L.

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Abstract

The invention provides a virus disaggregating agent and a method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen. The virus disaggregating agent is characterized in that each 100ml of disaggregating agent comprises 0.1-1ml of detergent, 0-20g of protein denaturant, 0.01-1ml of reducing agent, 0.1-1ml of fat solvent, and base liquid as remainder amount. The antigen-antibody complex obtained by the neutralization reaction of testing blood sample or antiserum and virus antigen is disaggregated into free components, or virus core antigen is fully exposed and the antigen reactivity of the virus core antigen is remained, thereby remarkably improving the sensitivity by comparing with the prior HCV core antigen detection method; in addition, the detection window period of the antigen is 49 days shorter than that of the anti-HCV antibody averagely, the situation appears within 1-2d after HCV-RNA appears, thereby efficiently shortening the window period of the blood serum before transformation, which is of important signification on improving the detection rate of affected person in the window period and the safety of blood transfusion and blood products. In the invention, the virus disaggregating agent is suitable for the disaggregation of common virus antigen-antibody complex including HCV and HAV (Hepatitis A Virus).

Description

technical field [0001] The invention relates to a virus splitting agent and a method for splitting a virus antigen-antibody complex with the splitting agent, so as to rapidly and accurately detect hepatitis C virus HCV antigen, belonging to the field of biotechnology. Background technique [0002] Hepatitis C virus HCV is a chronic hepatotropic virus. Except for some (about 10%-20%) of HCV-infected people who can recover by themselves, the vast majority will turn into chronic infection with a natural course of 20-30 years, of which about 20% of chronically infected people It will develop liver cirrhosis, and 20% of infected people will develop hepatocellular carcinoma. The incidence of chronicity is significantly higher than that of hepatitis B, and the mortality rate is higher. Therefore, the detection of hepatitis C virus HCV is of great significance for the early diagnosis of hepatitis C virus infection and guidance of clinical treatment. The existing detection technolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N21/31C07K16/10C07K16/06C07K1/22
CPCY02A50/30
Inventor 龙润乡谢忠平杨蓉白惠珠李华董丽娟蒋蕊鞠崔萍芳
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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