Rhodamine 6G-based mercury ion detection fluorescent probe molecule, preparation method and application

A fluorescent probe and mercury ion technology, which is applied in the field of mercury ion detection, can solve the problems of high detection cost, complicated sample preparation, and long test time, and achieve the effects of small toxic and side effects, good chemical stability, and simple synthesis method

Inactive Publication Date: 2016-06-08
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although many testing methods for mercury ions have high sensitivity, they generally have problems such as high testing costs, complicated sample preparation, and long testing time.

Method used

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  • Rhodamine 6G-based mercury ion detection fluorescent probe molecule, preparation method and application
  • Rhodamine 6G-based mercury ion detection fluorescent probe molecule, preparation method and application
  • Rhodamine 6G-based mercury ion detection fluorescent probe molecule, preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: Synthesis of fluorescent probe molecules based on rhodamine 6G for mercury ion detection

[0033] For specific synthetic routes, see figure 1 .

[0034] (1) Preparation of 2-formyl-8-hydroxyquinoline: 0.32 g of 2-methyl-8-hydroxyquinoline (2 mmol) was dissolved in 20 mL of dioxane. Heat to 60°C, then weigh 0.27g of SeO 2 It was added to the above solution, and then the temperature was raised to 80° C., and the reaction mixture was heated to reflux for 10 hours under the protection of nitrogen. After the reaction, the reaction liquid was cooled to room temperature, and the obtained precipitate was washed with 8 mL of dioxane and 8 mL of dichloromethane, filtered, and dried in vacuo. The obtained crude product was passed through a silica gel chromatography column and eluted with petroleum ether: ethyl acetate = 9:1 (v:v), and the eluate was concentrated to obtain 0.14 g of a light yellow compound. Yield 40.5%. 1 H-NMR (500MHz, CDCl 3):δ10.2(s,1H),8.31-...

Embodiment 2

[0037] Example 2: Selectivity of mercury ion detection fluorescent probe molecules based on rhodamine 6G to mercury ion ultraviolet detection

[0038] DMSO (dimethyl sulfoxide):HEPES (4-hydroxyethylpiperazineethanesulfonic acid buffer solution) (0.02mol / L, pH=7.4)=6:4(v:v) solution was used to control the experimental conditions.

[0039] The mercury ion detection fluorescent probe molecule based on rhodamine 6G is dissolved with the solvent of DMSO:HEPES=6:4 (v:v) and is settled in the volumetric flask of 100mL, is prepared as the fluorescent probe molecular concentration is 20 μ mol / L The solution.

[0040] Divide the sample vials into 12 groups, each sample vial in each group was added with 5mL concentration of 20 μmol / L DMSO based on rhodamine 6G mercury ion detection fluorescent probe molecule: HEPES (0.02mol / L, pH=7.4)=6 :4(v:v) solution, and then add 25 μL of Al with a concentration of 0.1mol / L 3+ , Na + ,Co 2+ , Ni 2+ ,Cu 2+ ,Zn 2+ ,Pb 2+ ,Cd 2+ , Ag + ,Fe 3...

Embodiment 3

[0042] Example 3: Selectivity of fluorescent probe molecules for mercury ion detection based on rhodamine 6G for mercury ion fluorescence detection

[0043] DMSO (dimethyl sulfoxide):HEPES (4-hydroxyethylpiperazineethanesulfonic acid buffer solution) (0.02mol / L, pH=7.4)=6:4(v:v) solution was used to control the experimental conditions.

[0044] The mercury ion detection fluorescent probe molecule based on rhodamine 6G is dissolved with the solvent of DMSO:HEPES=6:4 (v:v) and is settled in the volumetric flask of 100mL, is prepared as the fluorescent probe molecular concentration is 20 μ mol / L The solution.

[0045] Divide the sample vials into 12 groups, each sample vial in each group was added with 5mL concentration of 20 μmol / L DMSO based on rhodamine 6G mercury ion detection fluorescent probe molecule: HEPES (0.02mol / L, pH=7.4)=6 :4(v:v) solution, and then add 30 μL of Al with a concentration of 0.1mol / L 3+ , Na + ,Co 2+ , Ni 2+ ,Cu 2+ ,Zn 2+ ,Pb 2+ ,Cd 2+ , Ag + ...

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Abstract

The invention relates to a rhodamine 6G-based mercury ion detection fluorescent probe molecule; the fluorescent probe molecule has a structure as follows. The invention also discloses a preparation method of the rhodamine 6G-based mercury ion detection fluorescent probe molecule, and application thereof in mercury ion detection. The structure is shown in the description.

Description

technical field [0001] The invention belongs to the field of organic functional materials, and in particular relates to a rhodamine 6G-based fluorescent probe molecule for mercury ion detection, a preparation method and its application for mercury ion detection. Background technique [0002] Mercury is widely distributed in nature, and its extensive use causes damage to the ecology, causes pollution in the environment, and poses a great threat and impact on human health. Mercury is a poison that can accumulate in organisms. Inorganic mercury is easily converted into organic mercury. Both can be enriched in organisms. Accumulation in organisms and food chains can greatly increase the harmfulness of mercury to the human body. When mercury enters the human body, it accumulates in the liver, kidney, brain, heart, and bone marrow, causing neurotoxicity and deep tissue lesions, causing symptoms such as fatigue, dizziness, trembling, bleeding gums, baldness, numbness of hands and f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/107C09K11/06G01N21/64
CPCC07D491/107C09K11/06C09K2211/1022C09K2211/1033G01N21/64G01N21/6428G01N21/6486
Inventor 李雪梅周莹魏玉玲张俊峰徐济仓赵瑞瑞陈建华
Owner CHINA TOBACCO YUNNAN IND
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