Preparation method of melamine ratiometric fluorescent probe based on silver nanocluster compound

A technology of ratiometric fluorescent probes and silver nanoclusters, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of melamine ratiometric fluorescent probes, etc., and achieve low cost, simple preparation process, and high-quality products The effect of high sensitivity

Active Publication Date: 2018-10-12
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no domestic and foreign literature and patent reports based on DNA-stabilized silver nanoclusters and rhodamine 6G complexed melamine ratio fluorescent probes

Method used

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  • Preparation method of melamine ratiometric fluorescent probe based on silver nanocluster compound
  • Preparation method of melamine ratiometric fluorescent probe based on silver nanocluster compound
  • Preparation method of melamine ratiometric fluorescent probe based on silver nanocluster compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This example involves a method for preparing a melamine ratio fluorescent probe based on a DNA-stabilized silver nanocluster and rhodamine 6G complex. For the preparation process and the principle of melamine ratio fluorescence detection, see figure 1 As shown, the specific process steps are:

[0020] Preparation of DNA-stabilized silver nanoclusters: Add a certain amount of silver nitrate solution and DNA solution to 1 ml of twice-distilled water at 0°C, stir magnetically for 20 minutes to form a homogeneous mixture, and then add freshly prepared sodium borohydride Solution, vigorously stirred and reacted for 20 minutes in a dark place, wherein the molar concentrations of silver nitrate, DNA and sodium borohydride were 5 mmol / L, 200 micromol / L and 5 mmol / L respectively. The resulting product solution is filtered through a 0.4 micron filter membrane, and the filtrate is dialyzed through a dialysis bag with a molecular weight cut-off of 5000 Daltons to remove unreacted e...

Embodiment 2

[0025] The specific process steps of preparing DNA-stabilized silver nanoclusters in this example are the same as in Example 1, wherein the molar concentrations of silver nitrate, DNA and sodium borohydride are 8 mmol / L, 400 micromol / L and 7 mmol respectively / Lift. Dissolve the prepared silver nanoclusters in 200 microliters of twice distilled water, add 100 microliters of citrate aqueous buffer solution (pH 6.0) and mix well, then add 100 microliters of 0.2-1 nanomol / liter Rhodamine 6G The solution and the mixed solution were reacted in a dark place for 30 minutes, and the product solution was centrifuged, washed with ethanol and dried in vacuum to obtain a silver nanocluster / rhodamine 6G complex. The prepared complex was dispersed in 100 microliters of citrate aqueous buffer solution, the mass concentration of the complex was 1 to 4 mg / ml, incubated in a dark place for 30 minutes, and measured at different rhodamine 6G molar concentrations, The fluorescence emission spectr...

Embodiment 3

[0027] The specific process steps of preparing DNA-stabilized silver nanoclusters in this example are the same as in Example 1, wherein the molar concentrations of silver nitrate, DNA and sodium borohydride are 10 mmol / L, 600 micromol / L and 10 mmol respectively / Lift. Dissolve the prepared silver nanoclusters in 200 microliters of twice distilled water, add 100 microliters of citrate aqueous buffer solution (pH 6.5) and mix well, then add 100 microliters of 0.2-2 nanomoles / liter Rhodamine 6G The solution and the mixed solution were reacted in a dark place for 30 minutes, and the product solution was centrifuged, washed with ethanol and dried in vacuum to obtain a silver nanocluster / rhodamine 6G complex. The prepared complex was dispersed in 100 microliters of citrate aqueous buffer solution, the mass concentration of the complex was 2 to 5 mg / ml, incubated in a dark place for 30 minutes, and measured at different rhodamine 6G molar concentrations, The fluorescence emission sp...

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Abstract

The invention belongs to the technical field of preparation of precious metal nano materials and ratiometric fluorescent probes, and particularly relates to a preparation method of a melamine ratiometric fluorescent probe based on silver nanocluster and rhodamine 6G compounds with stable DNA (deoxyribonucleic acid). Electrostatic self-assembly is adopted for constructing a silver nanocluster/rhodamine 6G compound; melamine and thymine in the surface DNA of the silver nanocluster form a hydrogen bond to dissociate rhodamine 6G from the surface of the silver nanocluster, fluorescence resonance energy transfer is destroyed, and silver nanocluster fluorescence is recovered. The process has a slight impact on rhodamine 6G fluorescence, which serves as a reference signal, and silver nanoclusterfluorescence serves as a response signal. A linear relation between a ratio of a silver nanocluster to rhodamine 6G fluorescence emission peak intensity and a melamine molar concentration is fit to construct the melamine ratiometric fluorescent probe. The probe is simple in preparation process, low in cost and high in product sensitivity, and can develop into a novel melamine ratiometric fluorescent probe used for effectively detecting melamine in practical samples.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of noble metal nanomaterials and ratio fluorescent probes, in particular to a method for preparing a melamine ratio fluorescent probe based on a DNA-stabilized silver nanocluster and rhodamine 6G complex, and the prepared probe can be used for High sensitivity detection of melamine. Background technique: [0002] Melamine is a triazine nitrogen-containing heterocyclic organic compound with a nitrogen content of up to 66%, which is commonly used in many industries such as plastics, pesticides and fertilizers. Adding 1% melamine can increase the protein content in food by more than 4%. Some companies use melamine instead of protein to obtain high profits. The US Food and Drug Administration and the Chinese Ministry of Health set a safe limit of 1 mg / kg for infant formula and 2.5 mg / kg for adult food. Detecting the content of melamine in daily food is of great significance to people's healthy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/58C07D311/82G01N21/64
CPCC07D311/82C09K11/06C09K11/58C09K2211/1007C09K2211/1088G01N21/643G01N2021/6432C07D311/78C09K2211/1029
Inventor 桂日军付永鑫金辉卜祥宁
Owner QINGDAO UNIV
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