44 results about "Solid state culture" patented technology

The invention relates to a solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans, wherein a production method is a solid state fermental cultivation method of the mixed bacteria. A solid state fermentation culture medium is formed with enzymolysis bean pulp and rice bran as major ingredients and added with proper amount of an inorganic salt; the solid state fermentation culture medium is subpackaged and then sterilized; next, the solid state fermentation culture medium is inoculated with an isometric clostridium butyricum TK2 seed solution accounting for 10% of the total weight of the solid state culture medium and an isometric bacillus coagulans TQ33 seed solution accounting for 5% of the total weight of the solid state culture medium, and stirred evenly for fermentation. After the fermentation is carried out at 37 DEG C for 48 hours, a compound probiotics preparation of the clostridium butyricum TK2 and the bacillus coagulans TQ33 is obtained by drying at 50 DEG C, crushing and sieving; in the fermentation of the mixed bacteria, antinutritional factors in the bean pulp are decomposed more effectively under the mutualistic synergistic effect of the clostridium butyricum and the bacillus coagulans; as a result, nutrient substances in the fermentation bean pulp are richer and more balanced; therefore, a high protein feed rich in compound probiotics is provided.

The invention discloses a method for promoting orchid to grow and preventing diseases, and relates to a method for preventing the diseases and promoting orchid branches to grow and prolonging a flowering phase. The method disclosed by the invention comprises the following steps of uniformly mixing a solid state culture and a culture medium into a basin according to the mass ratio of 1 to 50 in orchid plant transplantation, wherein the solid state culture has the spore concentration s 1*10<7>-1*10<8> spore/g and prepared from Trichoderma harzianum T216, and planting an orchid plant, wherein the solid state culture can induce the resistance of the orchid plant to the fungoid diseases such as southern blight, rhizoctonia solani and anthracnose and bacterial soft rot caused by Erwinia carotovara during orchid growth, the plant morbidity is reduced, the disease index is reduced, the orchid is promoted to grow in advance, and the number of blades of the orchid are increased. In addition, the method disclosed by the invention has the advantages that a liquid state culture of the Trichoderma harzianum T216 is regulated into 1*10<5>-1*10<7> spore/mL when the orchid peeps a bud, and the spray treatment is carried out on the orchid plant, so that the branch number of the orchid is increased, and the flowering phase of the orchid is prolonged.

The invention discloses fa-tsai cell solid state culturing method. It solves the problem of relative fast growth. Its technical proposal is as follows: inoculating fa-tsai cell suspending liquid on solid state culture ground substance surface; spraying culture solution; culturing at manual control or natural condition; processing dry-wet rhythm culture; realizing full manual control or field enlarging culture. The technique increases breeding speed. The formed product has strong drought resistance and can preserve for long time; it can purify air and not produce poisoning matter; its enlarging culture can maintain ecological balance and reform desertification soil; thus it has high economic and environmental protection value.

The invention provides a Hickory chick beverage and the preparation method thereof. The preparation method comprises the steps of: mixing four solid materials with spinach juice aqueous solution to obtain a paste with the pH value being equal to 6.5-7.5, sterilizing to obtain a solid state culture medium, inoculating Hickory chick fruit body to a solid state culture medium for culturing at 23 plus or minus 2 DEG C for 15-20 days to obtain Hickory chick mycelia; boiling fresh soybean sprout or potato in boiled water for 20-30 minutes, collecting the filtrate, mixing with white sugar, peptone, and agar, decocting at 100 plus or minus 2 DEG C for 15-20 minutes, cooling to 15 plus or minus 2 DEG C to obtain a liquid culture medium, inoculating the Hickory chick thallus to the liquid culture medium, culturing and fermenting at 13 plus or minus 2 DEC C for 5-6 days to obtain fermenting solution base material for later use; and mixing with white sugar, citric acid, and purified water to obtain the beverage, wherein the solid culture medium comprises grain powder material, potassium dihydrogen phosphate, etc.

The invention discloses a processing technology of a single-cell protein from distilled spirit lees and relates to the technical field of animal nutrition and microbial fermentation. A finished product is obtained by performing pretreatment on the distilled spirit lees, then reasonably combining the lees with various ingredients according to a formula of a solid-state culture medium, fully mixing, then inoculating, fermenting for 48h, then drying and finally packaging. The processing technology comprises the steps of pretreatment of the lees, preparation of the solid-state culture medium, inoculation, fermentation, drying, packaging and obtainment of the finished product. The pretreatment comprises the steps that the distilled spirit lees is aired till the water content is 55-65%, then 600-1000 units of glucoamylase is added in per 100g of the distilled spirit lees, and the pretreatment of the lees is performed for 1-2h under the conditions that the temperature is 50-60 DEG C and the pH is 4.0-5.2. The processing technology disclosed by the invention can develop the distilled spirit lees into a single-cell protein fodder by combining with a microbial fermentation technology on the basis of animal nutrition theory, thereby turning waste into treasure and exploring fodder resources; simultaneously, the processing technology disclosed by the invention has the characteristics of simple technology, low cost and no pollution.

The invention discloses a method for preparing bacillus natto culture by using distiller's grains and its application, which belong to the technical field of fermentation engineering. The method comprises the following steps: bacterial species activation, primary seed culture, distiller's grains pretreatment, preparation of a fermentation medium, and solid fermentation to obtain the bacillus nattoculture. In the fermentation process of the present invention, the solid-state fermentation is used to produce bacillus natto. The entire fermentation process follows aseptic operation procedures, which enables solid-state culture production of bacillus natto to achieve pure-type culture and large-scale amplification production. The fermentation medium related to the invention solves the problemsof low number of viable bacteria existing in the fermentation with the distiller's grains as a main raw material, solves the problems of easy contamination of miscellaneous bacteria in liquid culture, low nutrient utilization rate, and difficult separation of bacteria and metabolites, and can greatly increase the yield of the bacillus natto.

The invention discloses a preparation method for brewing functional red yeast rice with low-yield citrinin. The method comprises the following steps of: culturing primary seeds by using red yeast rice strains, wherein 500ml of culture medium comprises 50g of glucose, 10 to 15g of peptone, 0.4 to 5g of NaNO3, 0.45g of MgSO4.7H2O and 0.45g of KH2PO4 according to the formula, and the pH value ranges from 4.8 to 5.0; holding 100 to 150ml of the culture medium with a 500ml triangular flask; sterilizing the culture medium for 30 minutes under the pressure of 1kg/sq.cm; cooling the culture medium and inoculating slant strains; culturing the slant strains for 45 to 50 hours at the temperature of 30 DEG C to obtain the primary seeds; and transferring the primary seeds on solid culture medium to perform solid-state culture to obtain the brewing functional red yeast rice with low-yield citrinin. In the method, the culture medium contains no citrinin, the Monacolin k content in the obtained brewing functional red yeast rice finished product can reach 5 to 14g/kg and the citrinin content is low.

The invention provides a processing method of vancomycin fermentation residue. The invention makes use of vancomycin fermentation residue as a sole culture medium, and the solid state culture method is adopted under the proper conditions for culturing yeast. The obtained yeast culture dry powder does not contain vancomycin residue after the culture and proper drying and smashing. Thus, the method not only solves the vancomycin residue problem in vancomycin fermentation residue and realizes the harm-free processing, but also can effectively cultures yeast and obtains a great deal of yeast protein with application value.

The invention tests liver protection and immune regulation functions by feeding a rat and a mouse with an antradiacomphora capsule which is prepared by adopting a special process and solid-state culture, wherein a liver protection test comprises the following steps of: inducing chronic hepatitis of the rat by using carbon tetrachloride twice a week for eight weeks, and measuring GPT and GOT, water content of liver, spleen and liver, liver protein and hydroxyproline content. The antradiacomphora capsule can lighten the rat chronic hepatitis induced by the carbon tetrachloride. A specific immunity test: the test matter is fed to the BALB/c mouse and egg albumen is injected, thereby accelerating the immune cell proliferation capacity and the effect of the immune regulation function generated by an antibody with serological specificity. A non-specific immunity test comprises the following step of: doing an oral test for consecutive six weeks by means of the BALB/c mouse. The invention accelerates the abdominal cavity phagocyte activity, regulates the anti-tumor cytohormone secretion and promotes the effect of the immune regulation function generated by a serological antibody.

The invention relates to a monascus compound preparation capable of regulating pressure and lipid and improving arteriosclerosis, coronary heart disease and cerebral embolism. The preparation is prepared through the following steps: fermenting ginseng and gingko leaves in a liquid state to obtain ginseng fermentation liquor and gingko fermentation liquor, culturing monascus 9901 bacterium or 9906strain in a liquid state, and adding water into the ginseng fermentation liquor, the gingko fermentation liquor and grains to prepare a solid-state culture medium; grafting and performing solid statefermentation to initially obtain a monascus strain; sterilizing malt juice or potato juice, sugar and agar to prepare a plate; grafting and culturing to prepare a pure monascus strain; preparing a strain enlarged culture fluid; grafting and performing strain enlarged culture; culturing in a shaker; performing sterilization grafting and culturing on the ginseng extract, the gingko extract and a solid fermentation nutrient solution; sterilizing, baking, crushing and uniformly mixing the fermented solid culture to obtain monascus fine powder; and preparing the preparation according to a corresponding preparation process of the preparation. The non-citrinin high-color value functional monascus produced by adopting a liquid-state fermentation technology in the invention has the advantages of low cost, edible safety, tiny toxic or side effect, stable and reliable curative effect and short course of treatment.

The invention discloses a tetramethylpyrazine bacillus amyloliquefaciens, and applications of a sesame-flavor baijiu special-purpose mouldy bran. The tetramethylpyrazine sesame-flavor baijiu special-purpose mouldy bran is obtained using tetramethylpyrazine bacillus amyloliquefaciens XJB-104 strain via following steps: preparation of a primary seed solution, preparation of a secondary seed solution, bran solid state culture medium fermentation, and aeration-drying of an obtained matured mouldy bran material. The obtained tetramethylpyrazine sesame-flavor baijiu special-purpose mouldy bran is bright in color, is dark brown, possesses typical smell, and slight ammonia smell, and is beneficial for improvement of the quality of sesame-flavor baijiu; and the tetramethylpyrazine content is higher than 380mg/kg.

The invention discloses a solid-state fermentation method for sugarcane fruit vinegar, which belongs to the technical field of brewing. The solid-state fermentation method comprises the following steps of: uniformly mixing bagasse, bran, and sugarcane juice with adjusted sugar degree and pH value, sterilizing and cooling, and then preparing a solid-state culture medium; inoculating a brewer yeast in a mass fraction of 0.5-1.0% and an aroma-producing yeast in a mass fraction of 0.5-1.0% in the solid-state culture medium respectively and performing alcoholic fermentation, controlling a material temperature to be 26-32 DEG C, and performing anaerobic fermentation for 5-7 days; performing airtight ageing on fermented grains for 10-15 days at an environmental temperature of 35-40 DEG C; then inoculating acetobacter xylinum in a mass fraction of 5-10%, controlling the material temperature to be 30-37 DEG C, performing aerobic fermentation for 8-12 days, and the obtaining mature vinegar substrate; and finally, performing vinegar pouring by a three-time nested pouring method, sterilizing the firstly-poured vinegar for 10-20 minutes at 60-70 DEG C, then performing filtration sterilization by an ultrafiltration membrane system, thus obtaining the sugarcane vinegar which is strong in aroma, and harmonious in vinegar aroma and fruit aroma.

The invention relates to a method using solid-state fermentation to prepare enterococcus faecalis. The method includes the steps of firstly, inoculating enterococcus faecalis seeds into a primary seed culture medium, and culturing at 35-40 DEG C under a micro-aerobic condition for 16-25 hours to obtain a primary seed solution; secondly, inoculating the primary seed solution obtained in the first step into a secondary seed culture medium of a seed tank, and performing amplification culture at 35-40 DEG C under a micro-aerobic condition for 16-20 hours to obtain a secondary seed solution; thirdly, inoculating the secondary seed solution obtained in the second step into the fermentation culture medium of a fermentation tank for culture, and fermenting at 35-40 DEG C under a micro-aerobic condition for 16-30 hours to obtain a tertiary seed solution; fourthly, evenly mixing the tertiary seed solution obtained in the third step with a solid-state culture medium, bagging, and performing fermentation culture for 36-80 hours to obtain a fresh fermentation material; fifthly, drying the fresh fermentation material obtained in the fourth step to obtain the enterococcus faecalis prepared by solid-state fermentation. Compared with the prior art, the method is simple in preparation process, low in production cost, high in viable count of the enterococcus faecalis, and the like.

The invention discloses a method for mechanically transplanting tissue culture seedlings, and belongs to the technical field of plant tissue cultivation. The method is applied to a strip tissue culture seedling cultured singly in a test tube, and is cultured by a solid-state culture medium in the test tube. The method is used for identifying and locating the node of a tissue culture seedling by utilizing machine vision technology and performing transplanting related operation on the tissue culture seedling by a robot according to the node location, and comprises the steps of seedling selection, cutting and implanting. The method can complete switching from manual transplanting operation to mechanical transplanting operation, so that the manual labor intensity can be reduced, a clean sterile environment without human disturbance is created, the pollution level is reduced, and the seedling survival rate is increased. The method has actual meaning.

The invention discloses a method for preparing a phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure by mixing beer wastewater sludge with bran, wherein the culture medium comprises the following components in parts by weight: 72-77 parts of the beer wastewater sludge and 23-28 parts of the bran. The method comprises the following steps: inoculating the culture medium with phosphate-solubilizing bacteria; carrying out solid-state culture in a microbial solid-state incubator at 28-32 DEG C, and ventilating and turning over materials every 9h. The method disclosed by the invention overcomes the shortcomings in the existing production culture medium for a phosphorus bacteria microbial fertilizer, raw materials of the culture medium are easily available, the composition of the culture medium is simple, the technological conditions in the production process are mild, and easy to control, equipment requirements are easy and feasible, the investment is small, the cost is low, the industrial application is facilitated, and beer wastewater treatment sludge which is solid waste discharged by breweries is recycled, so that the discharge of pollutants is reduced, the ecological environment is protected, and the economic benefit and social effect are produced.

The invention provides a method of producing exoinulinase with utilization of solid state fermentation of streptomyces grisepoplanus S501 and belongs to the technical field of microorganisms. The method comprises the following steps: preparing a bacterial suspension by freeze-dried powder of a strain S501 and aseptic water on a selective medium, culturing for 3-5d at the temperature of 28 DEG C, scraping spores, putting the spores into the aseptic water, diluting until the concentration of a spore suspension is 106cfu/mL, inoculating to a solid state culture medium, and carrying out static culture for 3-6d at the temperature of 28 DEG C; and after fermentation, adding deionized water, carrying out oscillating extraction for 1h, filtering, carrying out centrifugation on a filtrate for 20 minutes at the temperature of 4 DEG C and the rotational speed of 8000rpm/min to obtain a supernatant containing the exoinulinase, and treating the supernatant by virtue of the conventional freeze-drying method to prepare an inulinase preparation. According to the method provided by the invention, with wheat bran as a solid fermentation matrix and garlic husk powder which is cheap and easily acquired as a carbon source, the strain S501 is induced to produce the exoinulinase, the enzyme activity of the exoinulinase reaches up to 209.63 +/- 0.96U/g, and the defects of high cost, time and labor consumption in a liquid state fermentation method and non suitability in industrial production in the prior art with inulin as a carbon source are overcome.

The invention provides the microbe polysaccharides compounds of suppressing the activity of human promyelocytic leukemia cells (HL-60) and human hepatocellular carcinoma cell (Bel-7402), and also provides its preparation method, belonging to the biological medicine technology field. The invention comprises the following steps: adopting the Penixillium oxalicum strain (CGMCC No.0907), getting the thalline and spore by liquid and solid state culture method; extracting it by boiling-water, alkali, and boiling-water and alkali respectively, finally getting the microbe polysaccharides compounds. The half lethal concentration (IC50) of microbe polysaccharides compounds in HL-60 is 0.3-2.2ª–g/mL, and when the concentration is 6.0ª–g/mL, the HL-60 doesn't grow completely; the half lethal concentration (IC50) of microbe polysaccharides compounds in Bel-7402 is 120-150ª–g/mL, and when the concentration is 300ª–g/mL, the cell doesn't grow completely.

The invention discloses a microorganism solid-state culture system. The microorganism solid-state culture system comprises a microorganism solid-state culture device (100) and a fluid source (200), wherein the microorganism solid-state culture device comprises a box body (110), a first inlet pipe (120) and a discharge pipe (130); the first inlet pipe (120) is communicated with the fluid source (200); the microorganism solid-state culture device further comprises a sieve plate (140) with sieve meshes; the sieve plate (140) is arranged in the box body (110) and divides the internal space of the box body (110) into upper and lower layers; the first inlet pipe (120) is communicated with the lower layer of the box body (110); the box body (110) is communicated with the outside via the discharge pipe (130). Since the sieve plate is arranged at the bottom of the box body, sterile air or water vapor is introduced from the box body at the bottom of the system and rises up and passes through materials stacked on the sieve plate, so that temperature difference and humidity difference between the upper and lower layers of the materials are relatively small.

The invention discloses a method for preparing rice bran bioactive peptide through solid-state fermentation of mixed bacteria species. The method comprises the steps: performing solid-state fermentation and culture of rice bran by using mixed bacteria species of aspergillus niger AS3.410, AS3.350 and AS3.365, and aspergillus oryzae AS3.910, firstly, activating bevel bacteria species to generate a seed culture medium, then performing the combined inoculation of four hypha in the sterilized rice bran, performing solid-state culture, and then separating and drying to obtain the mixed rice bran. According to the invention, composite microorganism microbial populations, such as high-yield cellulase, starch enzymes, pectinase, glucamylase, fat enzymes and protease are selected and used, and the rice bran is directly fermented by a solid-state fermentation method, not only is the nutritional level of leavening improved, but also some bitter peptide groups are modified and reorganized, so that the peptide of the made rice bran has no bitterness or abnormal taste basically, the production technology is simplified, the production cost can be effectively reduced, pollution is avoided, and the method is suitable for the large scale industry production of the rice bran bioactive peptide.

The invention relates to the field of microorganisms and provides a preparation method of a botrytis cinerea agent. The preparation method comprises the following steps: (1) preparation of a solid-state culture medium; (2) inoculation of botrytis cinerea; (3) solid-state fermentation; (4) spore collection. The botrytis cinerea agent is produced by utilizing solid-state fermentation of wine lees, the wine lees can be recycled, and the production cost of the botrytis cinerea agent is greatly reduced; meanwhile, the physiological activity of the prepared botrytis cinerea agent is stable, the spore content of the botrytis cinerea agent reaches 10<8>-10<10>CFU/g, and the method is suitable for large-scale production operation.

The invention discloses a method for promoting solid state fermentation of monascus to produce monacolin K, and belongs to the technical field of microbial fermentation. The method comprises the following steps: adding cyclic adenosine monophosphate (cAMP) into a solid state culture medium in a solid state fermentation process of the monascus, wherein the optimal addition amount is 0.7 mM; inoculating an activated monascus seed solution; and performing temperature-variable fermentation to the end point. According to the method, the yield of the monacolin K as a monascus beneficial secondary metabolite can be stably improved, and the operation method is simple.

The invention discloses a deodorization type feed additive, a preparation method, a use method and an evaluation method. The feed additive is prepared through the following steps of performing solid-state culture on lactobacillus plantarum, bacillus licheniformis, natto bacillus and bacillus amyloliquefaciens, performing drying to obtain lactobacillus plantarum dry powder, bacillus licheniformis dry powder, natto bacillus dry powder and bacillus amyloliquefaciens dry powder, and mixing the lactobacillus plantarum dry powder with the bacillus licheniformis dry powder, the natto bacillus dry powder and the bacillus amyloliquefaciens dry powder in the weight ratio of the lactobacillus plantarum dry powder to the bacillus licheniformis dry powder, to the natto bacillus dry powder to the bacillus amyloliquefaciens dry powder being (1-3) to (1-6) to (1-6) to (1-3) so as to obtain the additive. An advanced solid-state fermentation technique is adopted, high-density culture is performed on thelactobacillus plantarum, the bacillus licheniformis, the natto bacillus and the bacillus amyloliquefaciens, and the additive is rich in various enzymes and metabolites, so that pathogenic bacteria inlivestock and poultry breeding environment and intestinal tracts can be effectively restrained, nutrient absorption is promoted, reproduction of harmful bacteria in excrement is restrained, macromolecular nitrogenous substances in the excrement can be rapidly decomposed, environment odor is reduced, the growth properties of livestock and poultry can be improved, and the feed additive is convenient to use and low in cost.

The invention provides an orchid growth accelerating method; in orchid corm suckering transplantation, a solid state culture applied with Trichoderma spp and an orchid culture matrix are uniformly mixed; in orchid budding, a liquid fermentation culture applied with Trichoderma spp is sprayed on the orchid plant; in orchid transplanting, the solid state culture and the cultivation matrix are uniformly mixed according to mass ratio: 1:30, and then the orchid is planted. The beneficial effects are that the method can accelerate the orchid growth, prevents the orchid from getting sick, and thus improving orchid quality and survival rate.

The invention discloses a Bacillus thuringiensis solid bottom fermentation and continuous transplantation production method which comprises, (1) mixing the liquid state bacterial with solid state culture medium, storing in fermentation apparatus for fermentation, (2) treating the fermentate as bacterial to carry out continuous transfer and second fermentation. The invention realizes increased production efficiency.