Method for preparing phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure

A technology for medium preparation and phosphate-solubilizing bacteria, applied in the field of microorganisms, can solve the problems of expensive raw materials, complex components, and high production costs, and achieve the effects of low cost, simple equipment requirements, and mild process control conditions.

Inactive Publication Date: 2014-07-30
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In the production method of mixing fungus chaff or sludge, corn flour and water as the main raw material of the medium, fungus chaff: corn flour: water = 25:5:70 (mass ratio), it is necessary to add

Method used

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  • Method for preparing phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure
  • Method for preparing phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure

Examples

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Effect test

Embodiment 1

[0030] Embodiment 1: Get 72 parts by weight of beer wastewater sludge and 28 parts by weight of harmless treatment and stir and mix evenly to prepare 4 kg of solid-state fermentation medium, and inoculate phosphobacteria seeds with 6% of the total amount of the medium After mixing, put it in a large-scale microbial solid-state incubator for cultivation, control the temperature between 28 and 32°C, ventilate and turn the material every 9 hours, and obtain phosphorus-solubilizing bacterial fertilizer after 6 days of cultivation, count the number of effective bacteria and measure the organic matter The content is shown in Table 1 below.

Embodiment 2

[0031] Embodiment 2: Get 77 parts by weight of beer wastewater sludge and 23 parts by weight of harmless treated beer wastewater and stir and mix evenly to prepare 4 kg of solid-state fermentation medium, and inoculate phosphobacteria seeds with 9% of the total amount of the medium After mixing, put it in a large-scale microbial solid-state incubator for cultivation, control the temperature between 28 and 32°C, ventilate and turn the material every 9 hours, and obtain phosphorus-solubilizing bacterial fertilizer after 6 days of cultivation, count the number of effective bacteria and measure the organic matter The content is shown in Table 1 below.

Embodiment 3

[0032] Embodiment 3: Get 75 parts by weight of beer waste water sludge and 25 parts by weight of harmless treatment and stir and mix evenly to prepare 4 kg of solid-state fermentation medium, and inoculate phosphobacteria seeds with 7.5% of the total amount of the medium After mixing, put it in a large-scale microbial solid-state incubator for cultivation, control the temperature between 28 and 32°C, ventilate and turn the material every 9 hours, and obtain phosphorus-solubilizing bacterial fertilizer after 6 days of cultivation, count the number of effective bacteria and measure the organic matter The content is shown in Table 1 below.

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Abstract

The invention discloses a method for preparing a phosphate-solubilizing bacterium culture medium and phosphate-solubilizing bacterial manure by mixing beer wastewater sludge with bran, wherein the culture medium comprises the following components in parts by weight: 72-77 parts of the beer wastewater sludge and 23-28 parts of the bran. The method comprises the following steps: inoculating the culture medium with phosphate-solubilizing bacteria; carrying out solid-state culture in a microbial solid-state incubator at 28-32 DEG C, and ventilating and turning over materials every 9h. The method disclosed by the invention overcomes the shortcomings in the existing production culture medium for a phosphorus bacteria microbial fertilizer, raw materials of the culture medium are easily available, the composition of the culture medium is simple, the technological conditions in the production process are mild, and easy to control, equipment requirements are easy and feasible, the investment is small, the cost is low, the industrial application is facilitated, and beer wastewater treatment sludge which is solid waste discharged by breweries is recycled, so that the discharge of pollutants is reduced, the ecological environment is protected, and the economic benefit and social effect are produced.

Description

technical field [0001] The invention relates to a preparation method of a phosphorus-dissolving bacterial culture medium and a phosphorus-dissolving bacterial fertilizer, belonging to the technical field of microorganisms. Background technique [0002] Phosphorus is one of the important elements necessary for plant growth and development, but 74% of the cultivated land in my country is deficient in phosphorus, and more than 95% of the phosphorus in the soil is invalid phosphorus, which is difficult for plants to use. For a long time, in order to increase the yield of crops, people have used a large amount of phosphorus fertilizers to meet the needs of plants for phosphorus in production. However, after phosphorus fertilizers are applied to the soil, insoluble phosphates are easily formed and quickly absorbed by soil minerals to become ineffective phosphorus. Plants It cannot be directly absorbed, resulting in low utilization rate of phosphorus, which seriously affects the gr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F5/00
CPCY02A40/20
Inventor 刘军刘小彬谢刚原海兵郇阿梅汪正斌
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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