Method for preparing bacillus natto culture by using distiller's grains and its application

Abstract

The invention discloses a method for preparing bacillus natto culture by using distiller's grains and its application, which belong to the technical field of fermentation engineering. The method comprises the following steps: bacterial species activation, primary seed culture, distiller's grains pretreatment, preparation of a fermentation medium, and solid fermentation to obtain the bacillus nattoculture. In the fermentation process of the present invention, the solid-state fermentation is used to produce bacillus natto. The entire fermentation process follows aseptic operation procedures, which enables solid-state culture production of bacillus natto to achieve pure-type culture and large-scale amplification production. The fermentation medium related to the invention solves the problemsof low number of viable bacteria existing in the fermentation with the distiller's grains as a main raw material, solves the problems of easy contamination of miscellaneous bacteria in liquid culture, low nutrient utilization rate, and difficult separation of bacteria and metabolites, and can greatly increase the yield of the bacillus natto.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a method for preparing bacillus natto culture by using distiller's grains and its application. Background technique [0002] With the development of the liquor industry, the utilization of distiller's grains, a by-product of liquor production, has become the focus of the industry. Solid distiller's grains are the main by-products of liquor production and the main pollutants in liquor production. Because raw materials such as sorghum and wheat are generally used, distiller's grains have high acidity and are rich in protein, amino acids, vitamins and various trace elements. Practice has proved that the degree of comprehensive utilization of distiller's grains directly affects the development of enterprises. If it is not handled properly, the pressure on the environment will be huge; and the level of distiller's grains processed feed is related to the implementatio...

AI Technical Summary

Problems solved by technology

In the high-density liquid fermentation industry of Bacillus natto, sucrose, yeast extract, tryptone, sodium chloride, etc. are generally used as the basic medium for the fermentation of Bacillus natto. From a production point of view, there is a unit medium The disadvantages such as low concentration of bacterial cells and high cost of bacterial liquid separation; at the same time, the purification of bacterial cells after liquid fermentation generally adopts freeze-drying or spray-drying methods to ensure high viable bacterial counts, but due to liquid fermentation Contains a lot of water, so complex pre-water removal steps are required to perform freeze-drying or spray-drying processes, which leads to extremely high costs

However, the research and application of solid-state fermentation in the fermentation of Bacillus natto is still immature, and there is no report on a relatively stable process that can produce high-quality Bacillus natto products, and the solid-state fermentation in industrial fermentation methods still has production environment requirements Strict, difficult and cumbersome disinfection of production materials, easy contamination of bacteria in the production process, low nutrient utilization, and difficult separation of bacteria and metabolites, etc.

[0006] Moreover, due to the complexity of microbial fermentation conditions, the fermentation conditions of different types of microbial bacteria are also very different, and they cannot learn from each other. Fermentation method and conditions for Bacillus natto with high bacterial count