Method for promoting solid state fermentation of monascus to produce monacolin K

A technology of solid-state fermentation and Monascus bacteria, applied in the field of microbial fermentation, can solve the problems of low yield and long production cycle

Inactive Publication Date: 2020-11-17
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, improved strains and various fermentation regulation strategies can regulate the production of beneficial SMs of Monascus to a certain extent, but they still cannot effectively solve the bottleneck problem of long industrial production cycle and low yield of MK.

Method used

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  • Method for promoting solid state fermentation of monascus to produce monacolin K
  • Method for promoting solid state fermentation of monascus to produce monacolin K

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Experimental program
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Effect test

Embodiment 1

[0020] Add the prepared 0.05M cAMP mother solution into the rice culture medium, so that the final cAMP concentration (mM, ie mmol / kg) is 0.7, 1, 2 (no cAMP is added as the control, CK), and the red yeast rice is prepared according to the instructions. Bacteria spore suspension, seed medium and rice medium preparation method and solid-state fermentation method, the spore suspension of Monascus MS-1 is inoculated in the seed medium at 30°C, 160r / min, cultivated for 36h, and then inoculated with 10% (v / w) The activated seed solution was cultivated at 30°C for 60 hours, and then cultivated at 24°C until the end of fermentation. Three parallel sets were set up to measure the content of MK in the fermentation product at 3, 6, 8, and 10 days respectively. The result is as figure 1 As shown, as the fermentation progressed, the MK production of all concentrations of cAMP reagent groups increased gradually. On the 10th day of fermentation, as the cAMP concentration increased, the MK ...

Embodiment 2

[0022] According to the Monascus spore suspension described in the instructions, the seed medium and the rice solid medium preparation method and the solid state fermentation method, the rice solid medium is packed in the solid state fermenter of 30L with a filling factor of 0.2, and the cAMP concentration is adjusted to be 0.7mM (without adding cAMP as the control, CK), sterilized at 115°C for 20min, before inoculation with 1.5mM 3 Dry at a wind speed of / h for about 3 hours, and then inoculate 10% (v / w) MS-1 seed solution cultivated at 30°C for 36 hours after thoroughly breaking up the materials, and add sterile water according to the humidity of the materials. The rotation speed was adjusted to 25r / min within 12 hours before fermentation, so that the medium and seed liquid were mixed quickly, and then stirred at 25r / min for 30min every day. After culturing at 30°C for 60h, transfer to 24°C and continue culturing until 10d. During the fermentation process, the ventilation v...

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Abstract

The invention discloses a method for promoting solid state fermentation of monascus to produce monacolin K, and belongs to the technical field of microbial fermentation. The method comprises the following steps: adding cyclic adenosine monophosphate (cAMP) into a solid state culture medium in a solid state fermentation process of the monascus, wherein the optimal addition amount is 0.7 mM; inoculating an activated monascus seed solution; and performing temperature-variable fermentation to the end point. According to the method, the yield of the monacolin K as a monascus beneficial secondary metabolite can be stably improved, and the operation method is simple.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a method for promoting monascus solid-state fermentation to produce monacolin K. Background technique [0002] Monascus spp., also known as Monascus spp., is an important filamentous fungus traditionally used for both medicine and food in my country. Its rice fermentation product, red yeast rice, has been used in my country for nearly two thousand years. Modern studies have shown that Monascus can produce beneficial secondary metabolites (SMs) such as Monacolin K (MK) and Monascus pigments, but some strains can also produce harmful SMs - citrinin (CIT, a mycotoxin). Among them, MK can regulate the synthesis of cholesterol in humans and animals. Therefore, red yeast rice with high content of MK has been developed into blood lipid-lowering drugs such as Xuezhikang and Zhibituo, as well as a variety of functional foods in China. At present, red yeast rice with high MK...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/66
CPCC12P17/06C12N1/145C12R2001/66
Inventor 陈福生谢玉梅
Owner HUAZHONG AGRI UNIV
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