Preparation method for blue-green algae single-cell protein feed

A single-cell protein, cyanobacteria technology, applied in animal feed, animal feed, applications, etc., can solve the problems that the feed industry and aquaculture industry are difficult to accept and promote, the product has high salt content, and the production cost is high, and achieves significant dehydration and energy saving effect. , the effect of rich fermentation products and high internal quality

Active Publication Date: 2014-04-30
宜兴市天石饲料有限公司
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AI Technical Summary

Problems solved by technology

[0010] However, there are two main technical problems in making cyanobacteria protein feed from the cyanobacteria produced by the "water bloom" of lake outbreaks: one is how to effectively utilize the nutritional components in cyanobacteria to transform them into cyanobacteria protein feed; the other is how to effectively remove Microcystin cyanotoxin MC
However, hydrolysis in strong acid and high temperature will destroy the tryptophan in the protein composition, and also partially oxidize cystine and methionine, and directly dry the neutralized hydrolyzate, which is very large, not only the salt content of the product High, and the production cost is very high, it is difficult for the feed industry and the breeding industry to accept promotion

Method used

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  • Preparation method for blue-green algae single-cell protein feed
  • Preparation method for blue-green algae single-cell protein feed
  • Preparation method for blue-green algae single-cell protein feed

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Embodiment 1

[0029] The preparation of embodiment 1 Aspergillus niger

[0030] The Aspergillus niger used in this application comes from the Institute of Microbiology, Chinese Academy of Sciences. After domesticating and adapting the strain to cyanobacteria, the applicant obtained a new strain that can improve single-cell protein, increase fermentation dehydration rate and resist miscellaneous bacteria pollution. Aspergillus niger, the applicant has deposited the new strain of Aspergillus niger in the General Microbiology Center of China Committee for the Collection of Microorganisms on December 27, 2013. The address is: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101, the deposit number is CGMCC No.8640.

Embodiment 2

[0031] Example 2 Preparation of Cyanobacteria Dehydration Filter Block

[0032] The cyanobacteria collection liquid with a water content above 98% is carried out by a pressurized dissolved air flotation machine for pressurized air flotation, and then press-filtered with a sludge filter press to obtain a cyanobacteria dehydration filter block with a water content of 79%-87.2%.

Embodiment 3

[0033] Embodiment 3 Cyanobacteria solid-state fermentation prepares single-cell protein feed

[0034] The medium involved in this embodiment:

[0035] The solid medium formula is bran and water with a mass ratio of 1-1.5;

[0036] Cha's medium formula: add 3g NaNO to 1L distilled water 3 , 1g K 2 HPO 4 , 0.5g KCL, 0.5g MgSO 4 ﹒ 7H 2 O, 0.01g FeSO 4 ﹒ 4H 2 O, 30g sucrose and 15-20g agar, adjust the pH to be 6.0-6.5.

[0037] Get the Aspergillus niger obtained in Example 1 and inoculate it on the Cha's culture medium for activation, culture it on a slope at 28-30°C for 3-5 days, after the slope is covered with colonies, pick a colony, and inoculate it on the Krebs In Pingcha's medium, expand the slope at 28-30°C for 3-4 days, wait until the slope is covered with colonies, add sterile water to scrape the colonies on the slope to make a bacterial suspension, and use 3ml of Aspergillus niger as a solution of Aspergillus niger. 1×10 6 -1×10 8 The cfu / ml Aspergillus ...

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Abstract

The invention provides a preparation method for a blue-green algae single-cell protein feed. According to the preparation method, aspergillus niger with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.8640 is used for carrying out solid-state fermentation on blue-green algae. The preparation method comprises the specific steps: (1) dehydrating the blue-green algae and carrying out filter pressing to prepare a blue-green algae dehydrated filtering block; (2) inoculating aspergillus niger liquid into a solid-state culture medium and culturing to obtain solid-state aspergillus niger; (3) adding the solid-state aspergillus niger into the blue-green algae dehydrated filtering block and mixing and fermenting to obtain a blue-green algae fermentation product; (4) drying the blue-green algae fermentation product by hot air, and crushing and sieving to obtain the blue-green algae single-cell protein feed. According to the preparation method for the blue-green algae single-cell protein feed, waste resources are utilized, the dehydration is energy-saving and the environmental pollution is not caused; the detoxification of micro-capsule blue-green algae toxin and the synchronous production of the single-cell protein feed are realized.

Description

technical field [0001] The invention relates to a waste resource feed utilization technology, in particular to a preparation method of cyanobacteria single-cell protein feed. technical background [0002] With the continuous and rapid development of industrial and agricultural production and the frequent intensification of human activities, the pollution of inland freshwater lakes has become increasingly serious. The low-level plant blue-green algae in the lakes will grow wildly every year from June to October, which is called "water bloom". ". "Water blooms" occur frequently, which have adverse effects on local social and economic development, residents' life and life health. Its frequent occurrence, persistence and severity have attracted great attention from the government and all walks of life. [0003] There are more than 300 common species of cyanobacteria in China, among which the dominant species is called Microcystis aeruyinvsa, and when "bloom" occurs in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/16
Inventor 薛宏基邹益东刘凤琴邹苏燕余锦华
Owner 宜兴市天石饲料有限公司
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